Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :7793911
Publication Date : //

Cloning and expression in Saccharomyces cerevisiae of a Trichoderma reesei beta-mannanase gene containing a cellulose binding domain.


beta-Mannanase (endo-1,4-beta-mannanase; mannan endo-1,4-beta-mannosidase; EC 3.2.1.78) catalyzes endo-wise hydrolysis of the backbone of mannan and heteromannans, including hemicellulose polysaccharides, which are among the major components of plant cell walls. The gene man1, which encodes beta-mannanase, of the filamentous fungus Trichoderma reesei was isolated from an expression library by using antiserum raised towards the earlier-purified beta-mannanase protein. The deduced beta-mannanase consists of 410 amino acids. On the basis of hydrophobic cluster analysis, the beta-mannanase was assigned to family 5 of glycosyl hydrolases (cellulase family A). The C terminus of the beta-mannanase has strong amino acid sequence similarity to the cellulose binding domains of fungal cellulases and is preceded by a serine-, threonine-, and proline-rich region. Consequently, the beta-mannanase is probably organized similarly to the T. reesei cellulases, having a catalytic core domain separated from the substrate-binding domain by an O-glycosylated linker. Active beta-mannanase was expressed and secreted by using the yeast Saccharomyces cerevisiae as the host. The results indicate that the man1 gene encodes the two beta-mannanases with different isoelectric points (pIs 4.6 and 5.4) purified earlier from T. reesei.

Authors : Stålbrand H , Saloheimo A , Vehmaanperä J , Henrissat B , Penttilä M ,

Related products :

Catalog number Product name Quantity
IC-1007 pEco-CMV-cHis-(Neo) PCR cloning kit: PCR cloning kit with a built-in mammalian expression vector (with Neomycin selection marker) in provided cloning cells, for mammalian expression of C-term His-tagg 10 x 50ul/ea
8203-0050 RABBIT ANTI SACCHAROMYCES CEREVISIAE, Product Type Polyclonal Antibody, Specificity SACCHAROMYCES CEREVISIAE, Target Species Yeast, Host Rabbit, Format Purified, Isotypes Polyclonal IgG, Applica 1 ml
SCH-8203-0050 RABBIT ANTI SACCHAROMYCES CEREVISIAE, Product Type Polyclonal Antibody, Specificity SACCHAROMYCES CEREVISIAE, Target Species Yeast, Host Rabbit, Format Purified, Isotypes Polyclonal IgG, Applica 1 ml
26-345 LCMT2 belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synth 0.05 mg
IC-1004 pEco-T7-nGST PCR cloning kit: PCR cloning kit with a built-in vector (T7 promoter based) in provided cloning cells for E Coli expression of N-term GST-tagged protein. 10 x 50ul/ea
IC-1001 pEco-T7-nHis PCR cloning kit: PCR cloning kit with a built-in vector (T7 promoter based) in provided cloning cells for E Coli expression of N-term His-tagged protein. 10 x 50ul/ea
IC-1006 pEco-T7-cHis PCR cloning kit: PCR cloning kit with a built-in vector (T7 promoter based) in provided cloning cells, for E Coli expression of C-term His-tagged protein. 10 x 50ul/ea
18-272-196783 Saccharomyces cerevisiae - Rabbit polyclonal to Saccharomyces cerevisiae Polyclonal 0.2 ml
18-272-196784 Saccharomyces cerevisiae - Rabbit polyclonal to Saccharomyces cerevisiae Polyclonal 0.5 ml
IC-1003 pEco-BAD-nHis PCR cloning kit: PCR cloning kit with a built-in vector (non-T7 promoter based) in provided cloning cells for E Coli expression of N-term His-tagged protein, specially designed for toxic 10 x 50ul/ea
M29429117 beta-Nicotinamide Adenine dinucleotide sodium salt from saccharomyces cerevisiae CAS: [20111-18-6] 25 g
EIAAB32304 Beta-interferon gene positive regulatory domain I-binding factor,BLIMP1,BLIMP-1,Homo sapiens,Human,Positive regulatory domain I-binding factor 1,PR domain zinc finger protein 1,PR domain-containing pr
IC-1002 pEco-suCMV-nHis-(Neo) PCR cloning kit: PCR cloning kit with a built-in mammalian expression vector (with neomycin selection marker) in provided cloning cells. The vector containing an engineered super 10 x 50ul/ea
18-003-44056 PR domain zinc finger protein 1 - PR domain-containing protein 1; Beta-interferon gene positive regulatory domain I-binding factor; BLIMP-1; Positive regulatory domain I-binding factor 1; PRDI-binding 0.1 mg Protein A
28-769 PRDM1 is a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcr 0.1 mg
84604-16-0 Saccharomyces cerevisiae Saccharomyces 1g
62-302 anti_Cdc37 (S. cerevisiae) antibody, rabbit serum Background Cdc37 was initially identified as a cell division cycle control protein of Saccharomyces cerevisiae (1) and was later found to have a much 100 ul
FYY201-1KT YLEX Expression Kit Gene Cloning Products *Yarrowia lipolytica Yeast Strain: Po1g, 1 stab *pYLEX1 - Expression Vector, 5 μg *pYLSC1 - Secretio
EIAAB32305 B lymphocyte-induced maturation protein 1,Beta-interferon gene positive regulatory domain I-binding factor,Blimp1,Blimp-1,Mouse,Mus musculus,PR domain zinc finger protein 1,PR domain-containing protei
FYY203-5MG pYLEX1 - Expression Vector Gene Cloning Products 5 μg
Y061740 Monoclonal Anti-Cellulose Binding Domain (CBDclos<_SUB>) produced in mouse Antibody 100ul
31-029 CDK6 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, 0.1 mg
30-430 METTL1 contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation.This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a con 0.1 mg
FYC006-20P T&A™ Cloning Kit + ECOS™ 9-5 (JM109) Gene Cloning Products FYC001-20P + FYE707-10VL x 2
FYC005-20P T&A™ Cloning Kit + ECOS™ 101 (DH5α) Gene Cloning Products FYC001-20P + FYE607-10VL x 2


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur