Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :7843284
Publication Date : //

Structure and growth-dependent regulation of the human cyclin B1 promoter.


As a step toward defining the signals important for the regulation of cyclin B1 expression, we cloned, sequenced, and partially characterized a 1012-bp genomic fragment encompassing the human cyclin B1 promoter. By transient expression experiments, we found that promoter activity resides within a -150/+182-bp DNA fragment. The activity of this promoter fragment was high in asynchronous NIH-3T3 cells, but dramatically decreased in quiescent cells. Time-course experiments, using stable transfectants expressing the CAT gene under the control of this fragment, were performed after releasing the cells from serum starvation. The results showed that the promoter becomes active at the end of the S phase and its activity increases during the cell cycle. Similar experiments performed with a shorter promoter region (-58/+182) showed that this 5' deletion mutant is active throughout the cell cycle. In good agreement with promoter activity, Northern analysis indicated that the endogenous gene is negatively regulated in quiescent murine NIH-3T3 cells. The data presented here demonstrate that in NIH-3T3 cells the cyclin B1 promoter is growth regulated, and important regulatory elements must exist in the region spanning -150 to -58 bp.

Authors : Piaggio G , Farina A , Perrotti D , Manni I , Fuschi P , Sacchi A , Gaetano C ,

Related products :

Catalog number Product name Quantity
EIAAB06559 CDI1,CDK2-associated dual-specificity phosphatase,CDKN3,CIP2,Cyclin-dependent kinase inhibitor 3,Cyclin-dependent kinase interactor 1,Cyclin-dependent kinase-interacting protein 2,Homo sapiens,Human,K
29-066 LZTS1 involved in the regulation of cell growth. LZTS1 may stabilize the active CDC2-cyclin B1 complex and thereby contribute to the regulation of the cell cycle and the prevention of uncontrolled cel 0.1 mg
orb82762 Human p16INK4a protein Recombinant Human Cyclin-Dependent Kinase Inhibitor 2A is a single non-glycosylated polypeptide chain containing 156 amino acids. Cyclin-dependent kinase inhibitors (CDKIs) are 1 mg
EIAAB35926 Cyclin-dependent kinase inhibitor 2B-related protein,Homo sapiens,Human,p15INK4B-related protein,P15RS,Regulation of nuclear pre-mRNA domain-containing protein 1A,RPRD1A
YSGKAMCC107E Cyclin Dependent Kinase 2 (cdk2), Cyclin Dependent Kinase 5 (cdk5), NO X w_Cyclin Dependent Kinase (cdk)1, ~33kD, Clone 8A12, Mab anti_Human, Mouse; WB 100 µg.
orb80779 Human cyclin-dependent kinase 4 protein Cyclin-Dependent Kinase Human Recombinant is expressed in E.coli as full-length protein having molecular weight of 38 kDa fused to an amino terminal hexahistidi 2
orb80780 Human cyclin-dependent kinase 8 protein Cyclin-Dependent Kinase Human Recombinant is expressed in E.coli as full-length protein fused to proprietary 16-Kd tag (a 6xHis tag is located at the very N-ter 2
28-099 In mammals, growth-dependent regulation of RNA polymerase I (Pol I) transcription is mediated by RRN3, an essential initiation factor. It interacts with Pol I in the absence of template DNA, augments 0.1 mg
EIAAB06368 CDK5 activator 2,CDK5R2,Cyclin-dependent kinase 5 activator 2,Cyclin-dependent kinase 5 regulatory subunit 2,Homo sapiens,Human,NCK5AI,p39,p39I
E1943h ELISA CDKN2C,CDKN6,Cyclin-dependent kinase 4 inhibitor C,Cyclin-dependent kinase 6 inhibitor,Homo sapiens,Human,p18-INK4c,p18-INK6 96T
U1943h CLIA CDKN2C,CDKN6,Cyclin-dependent kinase 4 inhibitor C,Cyclin-dependent kinase 6 inhibitor,Homo sapiens,Human,p18-INK4c,p18-INK6 96T
E1943h ELISA kit CDKN2C,CDKN6,Cyclin-dependent kinase 4 inhibitor C,Cyclin-dependent kinase 6 inhibitor,Homo sapiens,Human,p18-INK4c,p18-INK6 96T
U1943h CLIA kit CDKN2C,CDKN6,Cyclin-dependent kinase 4 inhibitor C,Cyclin-dependent kinase 6 inhibitor,Homo sapiens,Human,p18-INK4c,p18-INK6 96T
E0794h ELISA kit CDK4I,CDKN2,CDKN2A,Cyclin-dependent kinase 4 inhibitor A,Cyclin-dependent kinase inhibitor 2A, isoforms 1_2_3,Homo sapiens,Human,MTS1,MTS-1,Multiple tumor suppressor 1,p16-INK4,p16INK4A,p16 96T
U0794h CLIA CDK4I,CDKN2,CDKN2A,Cyclin-dependent kinase 4 inhibitor A,Cyclin-dependent kinase inhibitor 2A, isoforms 1_2_3,Homo sapiens,Human,MTS1,MTS-1,Multiple tumor suppressor 1,p16-INK4,p16INK4A,p16-INK4a 96T
EIAAB06568 CDKN1C,Cyclin-dependent kinase inhibitor 1C,Cyclin-dependent kinase inhibitor p57,Homo sapiens,Human,KIP2,p57Kip2
EIAAB06565 CDKN1B,Cyclin-dependent kinase inhibitor 1B,Cyclin-dependent kinase inhibitor p27,Homo sapiens,Human,KIP1,p27Kip1
E0794h ELISA CDK4I,CDKN2,CDKN2A,Cyclin-dependent kinase 4 inhibitor A,Cyclin-dependent kinase inhibitor 2A, isoforms 1_2_3,Homo sapiens,Human,MTS1,MTS-1,Multiple tumor suppressor 1,p16-INK4,p16INK4A,p16-INK4 96T
EIAAB35925 Bos taurus,Bovine,Cyclin-dependent kinase inhibitor 2B-related protein,p15INK4B-related protein,P15RS,Regulation of nuclear pre-mRNA domain-containing protein 1A,RPRD1A
EIAAB35927 Cyclin-dependent kinase inhibitor 2B-related protein,Kiaa4077,Mouse,Mus musculus,p15INK4B-related protein,P15rs,Regulation of nuclear pre-mRNA domain-containing protein 1A,Rprd1a
03-001-5 Epidermal Growth Factor (EGF), human, active EGF is a polypeptide growth hormone which plays an important role in the regulation of growth, proliferation, and differentiation of a wide range of cells 5 x 50 ug
03-001 Epidermal Growth Factor (EGF), human, active EGF is a polypeptide growth hormone which plays an important role in the regulation of growth, proliferation, and differentiation of a wide range of cells 50 ug
28-522 ANXA2 encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways 0.1 mg
28-523 The ANXA13 gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transductio 0.1 mg
28-524 ANXA13 encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathway 0.1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur