Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :8262987
Publication Date : //

Structural and functional importance of a conserved hydrogen bond network in human carbonic anhydrase II.


Amino acid substitutions at Thr199 of human carbonic anhydrase II (CAII) (Thr199-->Ser, Ala, Val, and Pro) were characterized to investigate the importance of a conserved hydrogen bonding network. The three-dimensional structures of azide-bound and sulfate-bound T199V CAIIs were determined by x-ray crystallographic methods at 2.25 and 2.4 A, respectively (final crystallographic R factors are 0.173 and 0.174, respectively). The CO2 hydrase activities of T199S and T199P variants suggest that the side chain methyl and backbone amino functionalities stabilize the transition state by approximately 0.4 and 0.8 kcal/mol, respectively. The side chain hydroxyl group causes: stabilization of zinc-hydroxide relative to zinc-water (pKa increases approximately 2 units); stabilization of the transition state for bicarbonate dehydration relative to the CAII.HCO3- complex (approximately 5 kcal/mol); and destabilization of the CAII.HCO3- complex (approximately 0.8 kcal/mol). An inverse correlation between log(kcatCO2/KM) and the pKa of zinc-water (r = 0.95, slope = -1) indicates that the hydrogen bonding network stabilizes the chemical transition state and zinc-hydroxide similarly. These data are consistent with the hydroxyl group of Thr199 forming a hydrogen bond with the transition state and a non-hydrogen-bonded van der Waals contact with CAII.HCO3-.

Authors : Krebs J F , Ippolito J A , Christianson D W , Fierke C A ,

Related products :

Catalog number Product name Quantity
EIAAB05175 CA6,Carbonate dehydratase VI,Carbonic anhydrase 6,Carbonic anhydrase VI,CA-VI,Homo sapiens,Human,Salivary carbonic anhydrase,Secreted carbonic anhydrase
EIAAB05176 Ca6,Car6,Carbonate dehydratase VI,Carbonic anhydrase 6,Carbonic anhydrase VI,CA-VI,Mouse,Mus musculus,Salivary carbonic anhydrase,Secreted carbonic anhydrase
EIAAB05177 Bos taurus,Bovine,CA6,Carbonate dehydratase VI,Carbonic anhydrase 6,Carbonic anhydrase VI,CA-VI,Salivary carbonic anhydrase,Secreted carbonic anhydrase
U1799h CLIA kit CA1,CAB,CA-I,Carbonate dehydratase I,Carbonic anhydrase 1,Carbonic anhydrase B,Carbonic anhydrase I,Homo sapiens,Human 96T
E0782h ELISA CA2,CAC,CA-II,Carbonate dehydratase II,Carbonic anhydrase 2,Carbonic anhydrase C,Carbonic anhydrase II,Homo sapiens,Human 96T
E1799h ELISA kit CA1,CAB,CA-I,Carbonate dehydratase I,Carbonic anhydrase 1,Carbonic anhydrase B,Carbonic anhydrase I,Homo sapiens,Human 96T
E1799h ELISA CA1,CAB,CA-I,Carbonate dehydratase I,Carbonic anhydrase 1,Carbonic anhydrase B,Carbonic anhydrase I,Homo sapiens,Human 96T
E0782h ELISA kit CA2,CAC,CA-II,Carbonate dehydratase II,Carbonic anhydrase 2,Carbonic anhydrase C,Carbonic anhydrase II,Homo sapiens,Human 96T
U0782h CLIA CA2,CAC,CA-II,Carbonate dehydratase II,Carbonic anhydrase 2,Carbonic anhydrase C,Carbonic anhydrase II,Homo sapiens,Human 96T
U1799h CLIA CA1,CAB,CA-I,Carbonate dehydratase I,Carbonic anhydrase 1,Carbonic anhydrase B,Carbonic anhydrase I,Homo sapiens,Human 96T
orb90049 Human Carbonic Anhydrase 2 protein Carbonic anhydrase Human Recombinant protein produced in E.coli containing 260 amino acids (1-260) and having a molecular mass of 29.2 kDa. The Carbonic anhydrase is 5
29-604 SMPX plays a role in the regulatory network through which muscle cells coordinate their structural and functional states during growth, adaptation, and repair. 0.1 mg
EIAAB05150 CA12,Carbonate dehydratase XII,Carbonic anhydrase 12,Carbonic anhydrase XII,CA-XII,Homo sapiens,Human,Tumor antigen HOM-RCC-3.1.3
EIAAB05173 CA5B,Carbonate dehydratase VB,Carbonic anhydrase 5B, mitochondrial,Carbonic anhydrase VB,CA-VB,Homo sapiens,Human
EIAAB05169 CA5,CA5A,Carbonate dehydratase VA,Carbonic anhydrase 5A, mitochondrial,Carbonic anhydrase VA,CA-VA,Homo sapiens,Human
EIAAB05156 CA14,Carbonate dehydratase XIV,Carbonic anhydrase 14,Carbonic anhydrase XIV,CA-XIV,Homo sapiens,Human,UNQ690_PRO1335
orb90047 Human Carbonic Anhydrase III His tag protein Carbonic anhydrase III Human Recombinant produced in E.coli, and having a molecular mass of 33.9 kDa. CAIII is expressed with an amino-terminal hexahistidi 5
orb90050 Human Carbonic Anhydrase-1 protein Recombinant Human Carbonic anhydrase produced in E.coli is a single, non-glycosylated polypeptide chain containing 281 amino acids (1-261 a.a) and having a molecular 5
EIAAB05160 CA3,CA-III,Carbonate dehydratase III,Carbonic anhydrase 3,Carbonic anhydrase III,Homo sapiens,Human
EIAAB05163 CA4,CA-IV,Carbonate dehydratase IV,Carbonic anhydrase 4,Carbonic anhydrase IV,Homo sapiens,Human
EIAAB05178 CA7,Carbonate dehydratase VII,Carbonic anhydrase 7,Carbonic anhydrase VII,CA-VII,Homo sapiens,Human
18-272-196830 Carbonic Anhydrase I (HRP) - Goat polyclonal to Carbonic Anhydrase I (HRP); EC 4.2.1.1; Carbonic anhydrase I; Carbonate dehydratase I; CA-I Polyclonal 20 mg
EIAAB05180 CA8,CALS,Carbonic anhydrase VIII,Carbonic anhydrase-related protein,CARP,CA-VIII,Homo sapiens,Human
EIAAB05170 CA Y,Ca5,Ca5a,Car5,Car5a,Carbonate dehydratase VA,Carbonic anhydrase 5A, mitochondrial,Carbonic anhydrase VA,CA-VA,Mouse,Mus musculus
EIAAB05183 Ca9,CAIX,CA-IX,Car9,Carbonate dehydratase IX,Carbonic anhydrase 9,Carbonic anhydrase IX,Membrane antigen MN homolog,Mouse,Mus musculus


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur