Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :8381958
Publication Date : //

Structural study of mutants of Escherichia coli ribonuclease HI with enhanced thermostability.


Systematic replacement of the amino acid residues in Escherichia coli ribonuclease HI with those in the thermophilic counterpart has revealed that two mutations, His62-->Pro (H62P) and Lys95-->Gly (K95G), increased the thermostability of the protein. These single-site mutant proteins, together with the mutant proteins His62-->Ala (H62A), Lys95-->Asn (K95N) and Lys95-->Ala (K95A), were crystallized and their structures were determined at 1.8 A resolution. The crystal structures of these mutant proteins reveal that only the local structure around each mutation site is essential for the increase in thermostability. For each mutant protein, the stabilization mechanism is considered to be as follows: (i) H62P is stabilized because of a decrease in the entropy of the unfolded state, without a change in the native backbone structure; (ii) K95G is stabilized since the strain caused by the left-handed backbone structure in the typical 3:5 type loop is eliminated; and (iii) K95N is slightly stabilized by a hydrogen bond formed between the side-chain N delta-atom of the mutated aspargine residue and the main-chain carbonyl oxygen within the same residue.

Authors : Ishikawa K , Kimura S , Kanaya S , Morikawa K , Nakamura H ,

Related products :

Catalog number Product name Quantity
B623 Minimal Agar USE : For isolation and characterization of nutritional mutants of Escherichia coli. 5x500gm
B623 Minimal Agar USE For isolation and characterization of nutritional mutants of Escherichia coli. Qty per Litre of Medium: 26,6
B624 Minimal Broth,Davis USE For isolatioan and characterization of nutritional mutants of Escherichia coli. Qty per Litre of Medium: 11,6
B624 Minimal Broth,Davis USE : For isolatioan and characterization of nutritional mutants of Escherichia coli. 5x500gm
B625 Minimal Broth,Davis w_o Dextrose USE : For isolation and characterization of nutritional mutants of Escherichia coli. 5x500gm
B625 Minimal Broth,Davis w_o Dextrose USE For isolation and characterization of nutritional mutants of Escherichia coli. Qty per Litre of Medium: 10,6
P90297Hu01 Ribonuclease A (RNASE1) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P93192Hu01 Ribonuclease A6 (RNASE6) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P87642Hu01 Ribonuclease A12 (RNASE12) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P93193Hu01 Ribonuclease A7 (RNASE7) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P93192Hu01 Ribonuclease A6 (RNASE6) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P93191Hu01 Ribonuclease A8 (RNASE8) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P87642Hu01 Ribonuclease A12 (RNASE12) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P84746Hu01 Ribonuclease A10 (RNASE10) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P90113Hu01 Ribonuclease T2 (RNASET2) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P93190Hu01 Ribonuclease A4 (RNASE4) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P93191Hu01 Ribonuclease A8 (RNASE8) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P84746Hu01 Ribonuclease A10 (RNASE10) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P93193Hu01 Ribonuclease A7 (RNASE7) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P93192Hu01 Ribonuclease A6 (RNASE6) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P84746Hu01 Ribonuclease A10 (RNASE10) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P90297Hu01 Ribonuclease A (RNASE1) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P90113Hu01 Ribonuclease T2 (RNASET2) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P90297Hu01 Ribonuclease A (RNASE1) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P90113Hu01 Ribonuclease T2 (RNASET2) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur