Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :8407802
Publication Date : //

Molecular cloning and characterization of acrA and acrE genes of Escherichia coli.


The DNA fragment containing the acrA locus of the Escherichia coli chromosome has been cloned by using a complementation test. The nucleotide sequence indicates the presence of two open reading frames (ORFs). Sequence analysis suggests that the first ORF encodes a 397-residue lipoprotein with a 24-amino-acid signal peptide at its N terminus. One inactive allele of acrA from strain N43 was shown to contain an IS2 element inserted into this ORF. Therefore, this ORF was designated acrA. The second downstream ORF is predicted to encode a transmembrane protein of 1,049 amino acids and is named acrE. Genes acrA and acrE are probably located on the same operon, and both of their products are likely to affect drug susceptibilities observed in wild-type cells. The cellular localizations of these polypeptides have been analyzed by making acrA::TnphoA and acrE::TnphoA fusion proteins. Interestingly, AcrA and AcrE share 65 and 77% amino acid identity with two other E. coli polypeptides, EnvC and EnvD, respectively. Drug susceptibilities in one acrA mutant (N43) and one envCD mutant (PM61) have been determined and compared. Finally, the possible functions of these proteins are discussed.

Authors : Ma D , Cook D N , Alberti M , Pon N G , Nikaido H , Hearst J E ,

Related products :

Catalog number Product name Quantity
B623 Minimal Agar USE For isolation and characterization of nutritional mutants of Escherichia coli. Qty per Litre of Medium: 26,6
B623 Minimal Agar USE : For isolation and characterization of nutritional mutants of Escherichia coli. 5x500gm
B624 Minimal Broth,Davis USE : For isolatioan and characterization of nutritional mutants of Escherichia coli. 5x500gm
B624 Minimal Broth,Davis USE For isolatioan and characterization of nutritional mutants of Escherichia coli. Qty per Litre of Medium: 11,6
IC-1001 pEco-T7-nHis PCR cloning kit: PCR cloning kit with a built-in vector (T7 promoter based) in provided cloning cells for E Coli expression of N-term His-tagged protein. 10 x 50ul/ea
IC-1006 pEco-T7-cHis PCR cloning kit: PCR cloning kit with a built-in vector (T7 promoter based) in provided cloning cells, for E Coli expression of C-term His-tagged protein. 10 x 50ul/ea
IC-1004 pEco-T7-nGST PCR cloning kit: PCR cloning kit with a built-in vector (T7 promoter based) in provided cloning cells for E Coli expression of N-term GST-tagged protein. 10 x 50ul/ea
B625 Minimal Broth,Davis w_o Dextrose USE For isolation and characterization of nutritional mutants of Escherichia coli. Qty per Litre of Medium: 10,6
B625 Minimal Broth,Davis w_o Dextrose USE : For isolation and characterization of nutritional mutants of Escherichia coli. 5x500gm
IC-1003 pEco-BAD-nHis PCR cloning kit: PCR cloning kit with a built-in vector (non-T7 promoter based) in provided cloning cells for E Coli expression of N-term His-tagged protein, specially designed for toxic 10 x 50ul/ea
PP-110L CyClone Ligase-free Cloning Kit, L pack Efficient plasmid construction for transformation of Escherichia coli 100Assays
PP-110S CyClone Ligase-free Cloning Kit, S pack Efficient plasmid construction for transformation of Escherichia coli 20Assays
P91177Mu01 Low Molecular Weight Kininogen (LMWK) Organism: Mus musculus (Mouse) Source: Escherichia coli 100ug
P91177Mu01 Low Molecular Weight Kininogen (LMWK) Organism: Mus musculus (Mouse) Source: Escherichia coli 10ug
P91177Mu01 Low Molecular Weight Kininogen (LMWK) Organism: Mus musculus (Mouse) Source: Escherichia coli 50ug
P91177Hu01 Low Molecular Weight Kininogen (LMWK) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P91177Hu01 Low Molecular Weight Kininogen (LMWK) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P91177Hu01 Low Molecular Weight Kininogen (LMWK) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P91805Hu02 High Molecular Weight Kininogen (HMWK) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P91805Hu01 High Molecular Weight Kininogen (HMWK) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
P91805Hu01 High Molecular Weight Kininogen (HMWK) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P91805Hu01 High Molecular Weight Kininogen (HMWK) Organism: Homo sapiens (Human) Source: Escherichia coli 50ug
P91805Hu02 High Molecular Weight Kininogen (HMWK) Organism: Homo sapiens (Human) Source: Escherichia coli 100ug
P91805Hu02 High Molecular Weight Kininogen (HMWK) Organism: Homo sapiens (Human) Source: Escherichia coli 10ug
ASC-ASH-0002 Molecular cloning reagents, PCR reagent: Taq DNA Polymerase 6x500U


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur