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Pubmed ID :8615692
Publication Date : //

Functional expression of uridine 5'-diphospho-glucose 4-epimerase (EC 5.1.3.2) from Arabidopsis thaliana in Saccharomyces cerevisiae and Escherichia coli.


It is our goal to investigate the biosynthesis of galactose-containing compounds in higher plants. Searching a database of expressed sequence tags, a cDNA from Arabidopsis thaliana (clone 108G20T7) with sequence similarity to UDP-glucose epimerase was identified and further analyzed. The 1356-bp-long cDNA included an open reading frame predicted to encode a 351 amino acid protein of 39 kDa. The presumed protein sequence showed a high degree of similarity to UDP-glucose epimerase sequences from bacteria, rat, and yeast. Complementation of the Saccharomyces cerevisiae gal1O mutant and expression of an active enzyme in Escherichia coli demonstrated that the cDNA encoded a functional UDP-glucose epimerase. The recombinant enzyme was purified to homogeneity. It showed a broad pH optimum of 7.0 to 9.5 and a Km of 0.11 mM. The UDP-glucose epimerase activity was not dependent on the addition of the cofactor NAD+ and was only moderately inhibited by high salt concentrations. Tissue-specific Northern analysis showed that the gene is expressed in all tissues of A. thaliana with highest expression levels in the stems and roots. Based on Southern analysis, there seems to be a single gene encoding UDP-glucose epimerase in A. thaliana. The cDNA analyzed during this study is the first known to encode a sugar-nucleotide modifying enzyme from higher plants. Its availability provides the means to investigate the role of UDP-glucose epimerase for the biosynthesis of UDP-galactose as precursor of galactolipids and cell wall polysaccharides.

Authors : Dörmann P , Benning C ,

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Catalog number Product name Quantity
U829957 Uridine 5'-Diphospho-α-D-glucose-13C6 Diammonium Salt C15H30N4O17P2 CAS: 904293-00-1 25 mg
AS07 258 Antibody: LOX-C | chloroplastic lipoxygenase, Immunogen: recombinant Arabidopsis thaliana protein, loop (aa 257-450) P38418, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 476 Antibody: AtArf2 | ADP ribosylation factor 2, Immunogen: synthetic peptide derived from Arabidopsis thaliana Q9SRC3, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 477 Antibody: S14 | 40S ribosomal protein S14-1, Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Q9SIH0, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS11 1783 Antibody: Acd1 | accelerated cell death 1 , Immunogen: recombinant PaO from Arabidopsis thaliana Q9FYC2, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 515 Antibody: KC1 | potassium channel KAT3 , Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS09 474 Antibody: AtMTP4 | metal tolerance protein B, Immunogen: synthetic peptide derived from Arabidopsis thaliana Q9SRC3, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 475 Antibody: Ligand-gated ion channel 1.3, Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Q9FH75, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Raph 100
AS10 931 Antibody: AtPCaP1 | Arabidopsis thaliana plasma membrane cation-binding protein-1, Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PCaP1 sequence Q96262, Host: rabbit, polyclonal, 100
AS10 651 Antibody: Enolase, Immunogen: recombinant Arabidopsis thaliana enolase At2g36530, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 671 Antibody: AGO5 | argonaute 5, Immunogen: peptide of Arabidopsis thaliana AGO5 Q9SJK3, AT2G27880, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 937 Antibody: CPLD11 , Immunogen: overexpressed fragment of recombinant Arabidopsis thaliana CPLD11 NP_566650, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 672 Antibody: AGO6 | argonaute 6, Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 At2g32940, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 459 Antibody: ClpB-P | ClpB3, Immunogen: KLH-conjugated peptide derived from ClpB-P of Arabidopsis thaliana Q9LF37, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 720 Antibody: PHOT1 | phototropin-1, Immunogen: KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT1 O48963, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS11 1628 Antibody: HSP18.5 | class IV heat shock protein, Immunogen: recombinant Arabidopsis thaliana Hsp18.5 , Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 200
AS10 721 Antibody: PHOT2 | phototropin-2, Immunogen: KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT2 P93025, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS11 1753 Antibody: HDT3 | histone deacetylase, Immunogen: recombinant part of Arabidopsis thaliana HDT3 Q9LZR5, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS06 116 Antibody: PGL35 | plastoglobulin 35, Immunogen: recombinant Arabidopsis thaliana PGL35 protein O81439 , Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Gossypium hirsutum cv. Del 200
AS09 527 Antibody: AGO1 | argonaute 1, Immunogen: N-terminal peptide of Arabidopsis thaliana AGO1 O04379, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana 100
AS09 614 Antibody: BiP | lumenal-binding protein , Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP2 Q39043. Chosen peptide is also conserved in Arabidopsis thaliana BiP1 prote 100
AS09 615 Antibody: BiP | lumenal-binding protein , Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP2 Q39043. Chosen peptide is also conserved in Arabidopsis thaliana BiP1 prote 100
AS08 346 Antibody: HSP90-1 | heat shock protein 90-1, Immunogen: full length recombinant Hsp90-1 from Arabidopsis thaliana AT5G52640, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana, Zea m 100
AS11 1629 Antibody: HSP90-2 | heat shock protein 90-2, Immunogen: full length recombinant Hsp90-2 from Arabidopsis thaliana, Host: rabbit, polyclonal, Confirmed reactivity: Arabidopsis thaliana 100
AS09 481 Antibody: BiP | lumenal-binding protein , Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP2 Q39043. Chosen peptide is also conserved in Arabidopsis thaliana BiP1 prote 200


 

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