Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :9128251
Publication Date : //

High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A.


We report that the actin assembly inhibitor latrunculin-A (LAT-A) causes complete disruption of the yeast actin cytoskeleton within 2-5 min, suggesting that although yeast are nonmotile, their actin filaments undergo rapid cycles of assembly and disassembly in vivo. Differences in the LAT-A sensitivities of strains carrying mutations in components of the actin cytoskeleton suggest that tropomyosin, fimbrin, capping protein, Sla2p, and Srv2p act to increase actin cytoskeleton stability, while End3p and Sla1p act to decrease stability. Identification of three LAT-A resistant actin mutants demonstrated that in vivo effects of LAT-A are due specifically to impairment of actin function and implicated a region on the three-dimensional actin structure as the LAT-A binding site. LAT-A was used to determine which of 19 different proteins implicated in cell polarity development require actin to achieve polarized localization. Results show that at least two molecular pathways, one actin-dependent and the other actin-independent, underlie polarity development. The actin-dependent pathway localizes secretory vesicles and a putative vesicle docking complex to sites of cell surface growth, providing an explanation for the dependence of polarized cell surface growth on actin function. Unexpectedly, several proteins that function with actin during cell polarity development, including an unconventional myosin (Myo2p), calmodulin, and an actin-interacting protein (Bud6/Aip3p), achieved polarized localization by an actin-independent pathway, revealing interdependence among cell polarity pathways. Finally, transient actin depolymerization caused many cells to abandon one bud site or mating projection and to initiate growth at a second site. Thus, actin filaments are also required for maintenance of an axis of cell polarity.

Authors : Ayscough K R , Stryker J , Pokala N , Sanders M , Crews P , Drubin D G ,

Related products :

Catalog number Product name Quantity
30-394 DSTN belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. It is the actin depolymerizing protein that severs actin 0.05 mg
28-124 EPLIN is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. EPLIN is a cytoskeleton-associated protein that inhibits actin filament d 0.05 mg
18-272-196322 smooth muscle Actin prediluted - Rabbit polyclonal to smooth muscle Actin prediluted; Alpha-actin-2; Cell growth-inhibiting gene 46 protein Polyclonal 7 ml
20-272-191692 alpha smooth muscle Actin - Mouse monoclonal [SPM332] to alpha smooth muscle Actin; Alpha-actin-2; Cell growth-inhibiting gene 46 protein Monoclonal 1 ml
E1342h ELISA ACTA2,Actin, aortic smooth muscle,ACTSA,ACTVS,Alpha-actin-2,Cell growth-inhibiting gene 46 protein,GIG46,Homo sapiens,Human 96T
U1342h CLIA ACTA2,Actin, aortic smooth muscle,ACTSA,ACTVS,Alpha-actin-2,Cell growth-inhibiting gene 46 protein,GIG46,Homo sapiens,Human 96T
E1342h ELISA kit ACTA2,Actin, aortic smooth muscle,ACTSA,ACTVS,Alpha-actin-2,Cell growth-inhibiting gene 46 protein,GIG46,Homo sapiens,Human 96T
20-272-190333 Actin - Mouse monoclonal [KJ43A] to Actin; Alpha-actin-1 Monoclonal 0.1 ml
20-272-191521 Actin - Mouse monoclonal [SPM160] to Actin; Alpha-actin-1 Monoclonal 1 ml
20-272-191632 alpha smooth muscle Actin - Mouse monoclonal [0.N.5] to alpha smooth muscle Actin; Alpha-actin-2; Cell growth-inhibiting gene 46 protein Monoclonal 0.25 ml
27-647 HS1 which is hematopoietic lineage cell-specific protein 1, is a substrate of protein tyrosine kinases in lymphocytes, it binds to F-actin, and promotes Arp2_3 complex-mediated actin polymerization. H 0.1 mg
30-789 CNN2, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. CNN2 could play a role in smooth muscle contraction and cell adhesi 0.05 mg
30-790 CNN2, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. CNN2 could play a role in smooth muscle contraction and cell adhesi 0.05 mg
27-160 Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, wh 0.05 mg
orb84841 Beta actin protein Beta actin protein recombinant produced in E.coli is non-glycosylated, Polypeptide chain containing 383 amino acids and having a molecular mass of 42.8 kDa. The beta actin protein i 0.5 mg
20-272-190331 Actin ( Cy3 ) - Mouse monoclonal [AC - 40] to Actin ( Cy3 ); Alpha-actin-1 Monoclonal 0.05 ml
20-272-190330 Actin - Mouse monoclonal [AC - 40] to Actin; Alpha-actin-1 Monoclonal 0.1 ml
29-775 Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activat 0.1 mg
20-272-191557 Actin. prediluted - Mouse monoclonal [SPM160] to Actin. prediluted; Alpha-actin-1 Monoclonal 7 ml
ACTB20-M Monoclonal Anti-smooth muscle, pan actin (6-isoforms of actin) IgG, ascites 100 ul
201-20-7135 Actin α _α-SMA{Actin alpha , smooth muscle aorta}rabbit.pAb 0.1ml
03-61075 Monoclonal antibody Anti-Cardiac Actin (fetal a-actin) 200 μl
'GTX76100 Actin (F-Actin) Clone 'NH3 antibody Isotype IgM Host Mouse 25
03-65175 Monoclonal antibody Anti-Cardiac Actin (fetal a-actin) 1 ml
ACTB20-M Monoclonal Anti_smooth muscle, pan actin (6_isoforms of actin) IgG, ascites 100 ul


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur