Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :9353280
Publication Date : //

Molecular heterogeneity of phospholipase D (PLD). Cloning of PLDgamma and regulation of plant PLDgamma, -beta, and -alpha by polyphosphoinositides and calcium.

Phospholipase D (PLD) has emerged as an important enzyme involved in signal transduction, vesicle trafficking, and membrane metabolism. This report describes the cloning and expression of a new Arabidopsis PLD cDNA, designated PLDgamma, and the regulation of PLDgamma, -beta, and -alpha by phosphatidylinositol 4,5-bisphosphate (PIP2) and Ca2+. The PLDgamma cDNA is 3.3 kilobases in length and codes for an 855-amino acid protein of 95,462 Da with a pI of 6.9. PLDgamma shares a 66% amino acid sequence identity with PLDbeta, but only a 41% identity with PLDalpha. A potential N-terminal myristoylation site is found in PLDgamma, but not in PLDalpha and -beta. Catalytically active PLDgamma was expressed in Escherichia coli, and its activity requires polyphosphoinositides. Both PLDgamma and -beta are most active at microM Ca2+ concentrations, whereas the optimal PLDalpha activity requires mM Ca2+ concentrations. Binding studies showed that the PLDs bound PIP2 in the order of PLDbeta > PLDgamma > PLDalpha. This binding ability correlates with the degree of conservation of a basic PIP2-binding motif located near the putative catalytic site. The binding of [3H]PIP2 was saturable and could be competitively decreased by addition of unlabeled PIP2. Neomycin inhibited the activities of PLDgamma and -beta, but not PLDalpha. These results demonstrate that PLD is encoded by a heterogeneous gene family and that direct polyphosphoinositide binding is required for the activities of PLDgamma and -beta, but not PLDalpha. The different structural and biochemical properties suggest that PLDalpha, -beta, and -gamma are regulated differently and may mediate unique cellular functions.

Authors : Qin W , Pappan K , Wang X ,

Related products :

Catalog number Product name Quantity
25-236 The L-type calcium channel is composed of four subunits alpha-1, alpha-2, beta and gamma. The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by 0.05 mg
28-223 The L-type calcium channel is composed of four subunits alpha-1, alpha-2, beta and gamma. The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by 0.1 mg
orb80615 Human TGF Beta 3, Plant protein TGFB3 Human Recombinant produced in plant is disulfide-linked homodimeric, glycosylated, polypeptide chain containing 118 amino acids and having a molecular mass of 27. 1
EIAAB31422 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1,Homo sapiens,Human,KIAA0581,Phosphoinositide phospholipase C-beta-1,Phospholipase C-beta-1,Phospholipase C-I,PLC-154,PLCB1,PLC-beta-1,P
EIAAB31424 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1,Phosphoinositide phospholipase C-beta-1,Phospholipase C-beta-1,Phospholipase C-I,PLC-154,Plcb1,PLC-beta-1,PLC-I,Rat,Rattus norvegicus
EIAAB31574 Calcium-independent phospholipase A2-gamma,Group VIB calcium-independent phospholipase A2,Intracellular membrane-associated calcium-independent phospholipase A2 gamma,iPLA2-gamma,Oryctolagus cuniculus
EIAAB31576 Calcium-independent phospholipase A2-gamma,Intracellular membrane-associated calcium-independent phospholipase A2 gamma,Ipla22,Ipla2g,iPLA2-gamma,Mouse,Mus musculus,Patatin-like phospholipase domain-c
EIAAB31432 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-4,Homo sapiens,Human,Phosphoinositide phospholipase C-beta-4,Phospholipase C-beta-4,PLCB4,PLC-beta-4
EIAAB31428 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3,Homo sapiens,Human,Phosphoinositide phospholipase C-beta-3,Phospholipase C-beta-3,PLCB3,PLC-beta-3
EIAAB31425 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2,Homo sapiens,Human,Phosphoinositide phospholipase C-beta-2,Phospholipase C-beta-2,PLCB2,PLC-beta-2
EIAAB31423 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1,Mouse,Mus musculus,Phosphoinositide phospholipase C-beta-1,Phospholipase C-beta-1,PLC-154,Plcb,Plcb1,PLC-beta-1
EIAAB31427 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2,Phosphoinositide phospholipase C-beta-2,Phospholipase C-beta-2,Plcb2,PLC-beta-2,Rat,Rattus norvegicus
EIAAB31426 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2,Mouse,Mus musculus,Phosphoinositide phospholipase C-beta-2,Phospholipase C-beta-2,Plcb2,PLC-beta-2
EIAAB31433 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-4,Bos taurus,Bovine,PCL-C1,Phosphoinositide phospholipase C-beta-4,Phospholipase C-beta-4,PLCB4,PLC-beta-4
EIAAB31430 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3,Mouse,Mus musculus,Phosphoinositide phospholipase C-beta-3,Phospholipase C-beta-3,Plcb3,PLC-beta-3
EIAAB31429 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3,Phosphoinositide phospholipase C-beta-3,Phospholipase C-beta-3,Plcb3,PLC-beta-3,Rat,Rattus norvegicus
EIAAB31421 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1,Bos taurus,Bovine,Phosphoinositide phospholipase C-beta-1,Phospholipase C-beta-1,PLC-154,PLCB1,PLC-beta-1
EIAAB31431 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-4,Phosphoinositide phospholipase C-beta-4,Phospholipase C-beta-4,Plcb4,PLC-beta-4,Rat,Rattus norvegicus
orb80386 Human Interferon-alpha 2a, Tobacco protein Interferon Alpha Human 2a Recombinant produced in Plant is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass o 5
orb81321 Human Serum Albumin, Plant protein HSA Human Recombinant produced in Plant is non-glycosylated, polypeptide chain containing 585 amino acids and having a molecular mass of 67 kDa.The optimum concentra 10 mg
EIAAB31575 BM-043,Calcium-independent phospholipase A2-gamma,Homo sapiens,Human,Intracellular membrane-associated calcium-independent phospholipase A2 gamma,IPLA22,iPLA2-2,IPLA2G,iPLA2-gamma,Patatin-like phospho
EIAAB29639 Calcium-dependent phospholipase A2,Group V phospholipase A2,Phosphatidylcholine 2-acylhydrolase 5,PLA2-10,Pla2g5,Rat,Rattus norvegicus
EIAAB29638 Calcium-dependent phospholipase A2,Group V phospholipase A2,Mouse,Mus musculus,Phosphatidylcholine 2-acylhydrolase 5,PLA2-10,Pla2g5
EIAAB29637 Calcium-dependent phospholipase A2,Group V phospholipase A2,Homo sapiens,Human,Phosphatidylcholine 2-acylhydrolase 5,PLA2-10,PLA2G5
EIAAB29627 cPLA2-beta,Cytosolic phospholipase A2 beta,Homo sapiens,Human,Phospholipase A2 group IVB,PLA2G4B


GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur

Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur



9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017


france@gentaur.com | Gentaur

Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U

spain@gentaur.com | Gentaur

ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636

GENTAUR Poland Sp. z o.o.

ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556


Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur