Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :9529113
Publication Date : //

Identification and temperature regulation of Legionella pneumophila genes involved in type IV pilus biogenesis and type II protein secretion.


Previously, we had isolated by transposon mutagenesis a Legionella pneumophila mutant that appeared defective for intracellular iron acquisition. While sequencing in the proximity of the mini-Tn10 insertion, we found a locus that had a predicted protein product with strong similarity to PilB from Pseudomonas aeruginosa. PilB is a component of the type II secretory pathway, which is required for the assembly of type IV pili. Consequently, the locus was cloned and sequenced. Within this 4-kb region were three genes that appeared to be organized in an operon and encoded homologs of P. aeruginosa PilB, PilC, and PilD, proteins essential for pilus production and type II protein secretion. Northern blot analysis identified a transcript large enough to include all three genes and showed a substantial increase in expression of this operon when L. pneumophila was grown at 30 degrees C as opposed to 37 degrees C. The latter observation was then correlated with an increase in piliation when bacteria were grown at the lower temperature. Southern hybridization analysis indicated that the pilB locus was conserved within L. pneumophila serogroups and other Legionella species. These data represent the first isolation of type II secretory genes from an intracellular parasite and indicate that the legionellae express temperature-regulated type IV pili.

Authors : Liles M R , Viswanathan V K , Cianciotto N P ,

Related products :

Catalog number Product name Quantity
25-105 ZFAT1 may be involved in transcriptional regulation. Overexpression of ZFAT1causes down-regulation of a number of genes involved in the immune response. Some genes are also up-regulated. 0.05 mg
28-193 The T-cell-specific transcription factor TCF7 activates genes involved in immune regulation and is a candidate locus for genetic susceptibility to type 1 diabetes. 0.05 mg
31-214 The T-cell-specific transcription factor TCF7 activates genes involved in immune regulation and is a candidate locus for genetic susceptibility to type 1 diabetes. 0.05 mg
28-192 The T-cell-specific transcription factor TCF7 activates genes involved in immune regulation and is a candidate locus for genetic susceptibility to type 1 diabetes. 0.05 mg
SCH-OBT0852 MOUSE ANTI LEGIONELLA PNEUMOPHILA, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, Applications P, Cl 0.1 mg
OBT0852 MOUSE ANTI LEGIONELLA PNEUMOPHILA, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, Applications P, Cl 0.1 mg
SCH-OBT0851 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUP 1, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, Applica 0.1 mg
OBT0851 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUP 1, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, Applica 0.1 mg
5625-0206 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUP 1, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgM, Applicat 0.2 mg
SCH-5625-0206 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUP 1, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgM, Applicat 0.2 mg
SCH-5625-0667 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUPS 1_9, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, Appl 0.2 mg
5625-0667 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUPS 1_9, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, Appl 0.2 mg
SCH-5625-0457 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUPS 4_10_14, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, 0.2 mg
SCH-5625-0086 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUP 1, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG2a, Applic 0.2 mg
5625-0056 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUP 1, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG1, Applica 0.2 mg
SCH-5625-0056 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUP 1, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG1, Applica 0.2 mg
5625-0066 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUP 1, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, Applica 0.2 mg
5625-0457 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUPS 4_10_14, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, 0.2 mg
5625-0086 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUP 1, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG2a, Applic 0.2 mg
SCH-5625-0066 MOUSE ANTI LEGIONELLA PNEUMOPHILA SEROGROUP 1, Product Type Monoclonal Antibody, Specificity LEGIONELLA PNEUMOPHILA, Target Species Bacterial, Host Mouse, Format Purified, Isotypes IgG3, Applica 0.2 mg
27-646 TBX21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmenta 0.05 mg
27-564 TBX19 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmenta 0.1 mg
27-565 TBX19 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmenta 0.05 mg
27-850 TBX5 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental 0.05 mg
27-848 TBX1 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental 0.1 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur