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Alpha-barbatene synthase (AtBS) (EC 4.2.3.69) (Beta-chamigrene synthase) (EC 4.2.3.78) (Terpeneoid synthase 11) (AtTPS11) (Thujopsene synthase) (EC 4.2.3.79)

 BARS_ARATH              Reviewed;         557 AA.
Q4KSH9; A3KPF7; Q9LU03;
28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
28-JUL-2009, sequence version 2.
13-FEB-2019, entry version 89.
RecName: Full=Alpha-barbatene synthase;
Short=AtBS;
EC=4.2.3.69;
AltName: Full=Beta-chamigrene synthase;
EC=4.2.3.78;
AltName: Full=Terpeneoid synthase 11;
Short=AtTPS11;
AltName: Full=Thujopsene synthase;
EC=4.2.3.79;
Name=BS; Synonyms=TPS11; OrderedLocusNames=At5g44630;
ORFNames=K15C23.7;
Arabidopsis thaliana (Mouse-ear cress).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
Arabidopsis.
NCBI_TaxID=3702;
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
PubMed=15965019; DOI=10.1104/pp.105.059386;
Wu S., Schoenbeck M.A., Greenhagen B.T., Takahashi S., Lee S.,
Coates R.M., Chappell J.;
"Surrogate splicing for functional analysis of sesquiterpene synthase
genes.";
Plant Physiol. 138:1322-1333(2005).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
[3]
GENOME REANNOTATION.
STRAIN=cv. Columbia;
PubMed=27862469; DOI=10.1111/tpj.13415;
Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
Town C.D.;
"Araport11: a complete reannotation of the Arabidopsis thaliana
reference genome.";
Plant J. 89:789-804(2017).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C.,
Ecker J.R.;
"Arabidopsis ORF clones.";
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
[5]
GENE FAMILY, AND NOMENCLATURE.
PubMed=12207221; DOI=10.1007/s00438-002-0709-y;
Aubourg S., Lecharny A., Bohlmann J.;
"Genomic analysis of the terpenoid synthase (AtTPS) gene family of
Arabidopsis thaliana.";
Mol. Genet. Genomics 267:730-745(2002).
[6]
TISSUE SPECIFICITY.
PubMed=12566586; DOI=10.1105/tpc.007989;
Chen F., Tholl D., D'Auria J.C., Farooq A., Pichersky E.,
Gershenzon J.;
"Biosynthesis and emission of terpenoid volatiles from Arabidopsis
flowers.";
Plant Cell 15:481-494(2003).
[7]
GENE FAMILY.
PubMed=12777052; DOI=10.1023/A:1023005504702;
Lange B.M., Ghassemian M.;
"Genome organization in Arabidopsis thaliana: a survey for genes
involved in isoprenoid and chlorophyll metabolism.";
Plant Mol. Biol. 51:925-948(2003).
[8]
FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES,
DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
PubMed=15918888; DOI=10.1111/j.1365-313X.2005.02417.x;
Tholl D., Chen F., Petri J., Gershenzon J., Pichersky E.;
"Two sesquiterpene synthases are responsible for the complex mixture
of sesquiterpenes emitted from Arabidopsis flowers.";
Plant J. 42:757-771(2005).
-!- FUNCTION: Involved in the biosynthesis of over 15 sesquiterpenes
(C15). The major products are (+)-alpha-barbatene (27.3%), (+)-
thujopsene (17.8%) and (+)-beta-chamigrene (9.9%). Can use
farnesyl diphosphate or geranyl diphosphate as substrates, but not
geranylgeranyl diphosphate. {ECO:0000269|PubMed:15918888,
ECO:0000269|PubMed:15965019}.
-!- CATALYTIC ACTIVITY:
Reaction=(2E,6E)-farnesyl diphosphate = (+)-alpha-barbatene +
diphosphate; Xref=Rhea:RHEA:29499, ChEBI:CHEBI:33019,
ChEBI:CHEBI:61690, ChEBI:CHEBI:175763; EC=4.2.3.69;
Evidence={ECO:0000269|PubMed:15918888};
-!- CATALYTIC ACTIVITY:
Reaction=(2E,6E)-farnesyl diphosphate = (+)-thujopsene +
diphosphate; Xref=Rhea:RHEA:30375, ChEBI:CHEBI:33019,
ChEBI:CHEBI:61737, ChEBI:CHEBI:175763; EC=4.2.3.79;
Evidence={ECO:0000269|PubMed:15918888};
-!- CATALYTIC ACTIVITY:
Reaction=(2E,6E)-farnesyl diphosphate = (+)-beta-chamigrene +
diphosphate; Xref=Rhea:RHEA:30379, ChEBI:CHEBI:33019,
ChEBI:CHEBI:61746, ChEBI:CHEBI:175763; EC=4.2.3.78;
Evidence={ECO:0000269|PubMed:15918888};
-!- COFACTOR:
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=1.2 uM for farnesyl diphosphate
{ECO:0000269|PubMed:15918888};
KM=0.21 uM for geranyl diphosphate
{ECO:0000269|PubMed:15918888};
Note=The formation of monoterpene products from geranyl
diphosphate (GPP) is unlikely, as the protein is not expected to
be present in plastids, where GPP is thought to be produced.;
-!- PATHWAY: Secondary metabolite biosynthesis; terpenoid
biosynthesis.
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
-!- TISSUE SPECIFICITY: Expressed exclusively in flowers. Expressed in
intrafloral nectaries and in the funiculus within the ovules.
{ECO:0000269|PubMed:12566586, ECO:0000269|PubMed:15918888}.
-!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important
for the catalytic activity, presumably through binding to Mg(2+).
-!- DISRUPTION PHENOTYPE: Does not emit any sesquiterpene volatiles
except (E)-beta-caryophyllene, alpha-copaene and alpha-humulene.
{ECO:0000269|PubMed:15918888}.
-!- SIMILARITY: Belongs to the terpene synthase family. Tpsa
subfamily. {ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=BAA98116.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
-----------------------------------------------------------------------
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Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; AY876386; AAX59990.1; -; mRNA.
EMBL; AB024024; BAA98116.1; ALT_SEQ; Genomic_DNA.
EMBL; CP002688; AED95140.1; -; Genomic_DNA.
EMBL; BT030324; ABO09887.1; -; mRNA.
RefSeq; NP_199276.1; NM_123830.3.
UniGene; At.22345; -.
ProteinModelPortal; Q4KSH9; -.
SMR; Q4KSH9; -.
STRING; 3702.AT5G44630.1; -.
iPTMnet; Q4KSH9; -.
PaxDb; Q4KSH9; -.
EnsemblPlants; AT5G44630.1; AT5G44630.1; AT5G44630.
GeneID; 834491; -.
Gramene; AT5G44630.1; AT5G44630.1; AT5G44630.
KEGG; ath:AT5G44630; -.
Araport; AT5G44630; -.
TAIR; locus:2152155; AT5G44630.
eggNOG; ENOG410JVXX; Eukaryota.
eggNOG; ENOG41105GB; LUCA.
HOGENOM; HOG000232971; -.
InParanoid; Q4KSH9; -.
KO; K15799; -.
OMA; YNHREGK; -.
OrthoDB; 360509at2759; -.
PhylomeDB; Q4KSH9; -.
BioCyc; ARA:AT5G44630-MONOMER; -.
BioCyc; MetaCyc:AT5G44630-MONOMER; -.
BRENDA; 4.2.3.69; 399.
BRENDA; 4.2.3.78; 399.
BRENDA; 4.2.3.79; 399.
SABIO-RK; Q4KSH9; -.
UniPathway; UPA00213; -.
PRO; PR:Q4KSH9; -.
Proteomes; UP000006548; Chromosome 5.
Genevisible; Q4KSH9; AT.
GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0009506; C:plasmodesma; IDA:TAIR.
GO; GO:0102878; F:(+)-alpha-barbatene synthase activity; IEA:UniProtKB-EC.
GO; GO:0102883; F:(+)-beta-chamigrene synthase activity; IEA:UniProtKB-EC.
GO; GO:0102879; F:(+)-thujopsene synthase activity; IEA:UniProtKB-EC.
GO; GO:0009975; F:cyclase activity; IBA:GO_Central.
GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
GO; GO:0051762; P:sesquiterpene biosynthetic process; IDA:TAIR.
GO; GO:0016106; P:sesquiterpenoid biosynthetic process; IDA:TAIR.
Gene3D; 1.10.600.10; -; 1.
Gene3D; 1.50.10.130; -; 1.
InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
InterPro; IPR034741; Terpene_cyclase_like_1_C.
InterPro; IPR001906; Terpene_synth_N.
InterPro; IPR036965; Terpene_synth_N_sf.
InterPro; IPR005630; Terpene_synthase_metal-bd.
InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
Pfam; PF01397; Terpene_synth; 1.
Pfam; PF03936; Terpene_synth_C; 1.
SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
SUPFAM; SSF48239; SSF48239; 1.
SUPFAM; SSF48576; SSF48576; 1.
1: Evidence at protein level;
Complete proteome; Cytoplasm; Lyase; Magnesium; Manganese;
Metal-binding; Reference proteome.
CHAIN 1 557 Alpha-barbatene synthase.
/FTId=PRO_0000380673.
MOTIF 310 314 DDXXD motif.
METAL 310 310 Magnesium or manganese 1. {ECO:0000250}.
METAL 310 310 Magnesium or manganese 2. {ECO:0000250}.
METAL 314 314 Magnesium or manganese 1. {ECO:0000250}.
METAL 314 314 Magnesium or manganese 2. {ECO:0000250}.
METAL 454 454 Magnesium or manganese 3. {ECO:0000250}.
METAL 462 462 Magnesium or manganese 3. {ECO:0000250}.
CONFLICT 162 162 F -> Y (in Ref. 1; AAX59990).
{ECO:0000305}.
SEQUENCE 557 AA; 64909 MW; 05439A5144A37240 CRC64;
MEALGNFDYE SYTNFTKLPS SQWGDQFLKF SIADSDFDVL EREIEVLKPK VRENIFVSSS
TDKDAMKKTI LSIHFLDSLG LSYHFEKEIE ESLKHAFEKI EDLIADENKL HTISTIFRVF
RTYGYYMSSD VFKIFKGDDG KFKESLIEDV KGMLSFYEAV HFGTTTDHIL DEALSFTLNH
LESLATGRRA SPPHISKLIQ NALHIPQHRN IQALVAREYI SFYEHEEDHD ETLLKLAKLN
FKFLQLHYFQ ELKTITMWWT KLDHTSNLPP NFRERTVETW FAALMMYFEP QFSLGRIMSA
KLYLVITFLD DACDTYGSIS EVESLADCLE RWDPDYMENL QGHMKTAFKF VMYLFKEYEE
ILRSQGRSFV LEKMIEEFKI IARKNLELVK WARGGHVPSF DEYIESGGAE IGTYATIACS
IMGLGEIGKK EAFEWLISRP KLVRILGAKT RLMDDIADFE EDMEKGYTAN ALNYYMNEHG
VTKEEASREL EKMNGDMNKI VNEECLKITT MPRRILMQSV NYARSLDVLY TADDVYNHRE
GKLKEYMRLL LVDPILL


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