GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Dual-specificity RNA methyltransferase RlmN (EC 2.1.1.192) (23S rRNA (adenine(2503)-C(2))-methyltransferase) (23S rRNA m2A2503 methyltransferase) (Ribosomal RNA large subunit methyltransferase N) (tRNA (adenine(37)-C(2))-methyltransferase) (tRNA m2A37 methyltransferase)

 A0A1Y1R4Q2_9GAMM        Unreviewed;       383 AA.
A0A1Y1R4Q2;
30-AUG-2017, integrated into UniProtKB/TrEMBL.
30-AUG-2017, sequence version 1.
16-JAN-2019, entry version 6.
RecName: Full=Dual-specificity RNA methyltransferase RlmN {ECO:0000256|HAMAP-Rule:MF_01849};
EC=2.1.1.192 {ECO:0000256|HAMAP-Rule:MF_01849};
AltName: Full=23S rRNA (adenine(2503)-C(2))-methyltransferase {ECO:0000256|HAMAP-Rule:MF_01849};
AltName: Full=23S rRNA m2A2503 methyltransferase {ECO:0000256|HAMAP-Rule:MF_01849};
AltName: Full=Ribosomal RNA large subunit methyltransferase N {ECO:0000256|HAMAP-Rule:MF_01849};
AltName: Full=tRNA (adenine(37)-C(2))-methyltransferase {ECO:0000256|HAMAP-Rule:MF_01849};
AltName: Full=tRNA m2A37 methyltransferase {ECO:0000256|HAMAP-Rule:MF_01849};
Name=rlmN {ECO:0000256|HAMAP-Rule:MF_01849};
ORFNames=B0D91_07975 {ECO:0000313|EMBL:OQX36921.1};
Oceanospirillales bacterium LUC14_002_19_P2.
Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales;
unclassified Oceanospirillales.
NCBI_TaxID=1940822 {ECO:0000313|EMBL:OQX36921.1};
[1] {ECO:0000313|EMBL:OQX36921.1}
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=LUC14_002_19_P2 {ECO:0000313|EMBL:OQX36921.1};
Lim S.J., Davis B.G., Gill D.E., Engel A.S., Anderson L.C.,
Campbell B.J.;
"Novel co-symbiosis in the unique lucinid bivalve Phacoides
pectinatus.";
Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
-!- FUNCTION: Specifically methylates position 2 of adenine 2503 in
23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503
modification seems to play a crucial role in the proofreading step
occurring at the peptidyl transferase center and thus would serve
to optimize ribosomal fidelity. {ECO:0000256|HAMAP-Rule:MF_01849}.
-!- CATALYTIC ACTIVITY:
Reaction=adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-
[ferredoxin] + 2 S-adenosyl-L-methionine = 2-
methyladenosine(2503) in 23S rRNA + 5'-deoxyadenosine + L-
methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-
homocysteine; Xref=Rhea:RHEA:42916, Rhea:RHEA-COMP:10000,
Rhea:RHEA-COMP:10001, Rhea:RHEA-COMP:10152, Rhea:RHEA-
COMP:10282, ChEBI:CHEBI:17319, ChEBI:CHEBI:33737,
ChEBI:CHEBI:33738, ChEBI:CHEBI:57844, ChEBI:CHEBI:57856,
ChEBI:CHEBI:59789, ChEBI:CHEBI:74411, ChEBI:CHEBI:74497;
EC=2.1.1.192; Evidence={ECO:0000256|HAMAP-Rule:MF_01849,
ECO:0000256|SAAS:SAAS01114928};
-!- CATALYTIC ACTIVITY:
Reaction=adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] +
2 S-adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-
deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin]
+ S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:43332, Rhea:RHEA-
COMP:10000, Rhea:RHEA-COMP:10001, Rhea:RHEA-COMP:10162,
Rhea:RHEA-COMP:10485, ChEBI:CHEBI:17319, ChEBI:CHEBI:33737,
ChEBI:CHEBI:33738, ChEBI:CHEBI:57844, ChEBI:CHEBI:57856,
ChEBI:CHEBI:59789, ChEBI:CHEBI:74411, ChEBI:CHEBI:74497;
EC=2.1.1.192; Evidence={ECO:0000256|HAMAP-Rule:MF_01849,
ECO:0000256|SAAS:SAAS01114934};
-!- COFACTOR:
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
Evidence={ECO:0000256|HAMAP-Rule:MF_01849};
Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
cysteines and an exchangeable S-adenosyl-L-methionine.
{ECO:0000256|HAMAP-Rule:MF_01849};
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01849,
ECO:0000256|SAAS:SAAS00385725}.
-!- MISCELLANEOUS: Reaction proceeds by a ping-pong mechanism
involving intermediate methylation of a conserved cysteine
residue. {ECO:0000256|HAMAP-Rule:MF_01849}.
-!- SIMILARITY: Belongs to the radical SAM superfamily. RlmN family.
{ECO:0000256|HAMAP-Rule:MF_01849, ECO:0000256|SAAS:SAAS00571858}.
-!- CAUTION: The sequence shown here is derived from an
EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
preliminary data. {ECO:0000313|EMBL:OQX36921.1}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; MUIA01000213; OQX36921.1; -; Genomic_DNA.
GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0070040; F:rRNA (adenine-C2-)-methyltransferase activity; IEA:UniProtKB-UniRule.
GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
GO; GO:0002935; F:tRNA (adenine-C2-)-methyltransferase activity; IEA:UniProtKB-UniRule.
GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
GO; GO:0070475; P:rRNA base methylation; IEA:UniProtKB-UniRule.
Gene3D; 3.20.20.70; -; 1.
HAMAP; MF_01849; RNA_methyltr_RlmN; 1.
InterPro; IPR013785; Aldolase_TIM.
InterPro; IPR040072; Methyltransferase_A.
InterPro; IPR027492; RNA_MTrfase_RlmN.
InterPro; IPR004383; rRNA_lsu_MTrfase_RlmN/Cfr.
InterPro; IPR007197; rSAM.
PANTHER; PTHR30544; PTHR30544; 1.
Pfam; PF04055; Radical_SAM; 1.
PIRSF; PIRSF006004; CHP00048; 1.
SFLD; SFLDF00275; adenosine_C2_methyltransferase; 1.
TIGRFAMs; TIGR00048; rRNA_mod_RlmN; 1.
3: Inferred from homology;
4Fe-4S {ECO:0000256|HAMAP-Rule:MF_01849,
ECO:0000256|SAAS:SAAS00463035};
Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01849,
ECO:0000256|SAAS:SAAS00462865};
Disulfide bond {ECO:0000256|HAMAP-Rule:MF_01849,
ECO:0000256|SAAS:SAAS00721829};
Iron {ECO:0000256|HAMAP-Rule:MF_01849, ECO:0000256|SAAS:SAAS00463035};
Iron-sulfur {ECO:0000256|HAMAP-Rule:MF_01849,
ECO:0000256|SAAS:SAAS00463035};
Metal-binding {ECO:0000256|HAMAP-Rule:MF_01849,
ECO:0000256|SAAS:SAAS00463035};
Methyltransferase {ECO:0000256|HAMAP-Rule:MF_01849,
ECO:0000256|SAAS:SAAS00462825, ECO:0000313|EMBL:OQX36921.1};
rRNA processing {ECO:0000256|HAMAP-Rule:MF_01849,
ECO:0000256|SAAS:SAAS00536180};
S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_01849,
ECO:0000256|SAAS:SAAS00462941};
Transferase {ECO:0000256|HAMAP-Rule:MF_01849,
ECO:0000256|SAAS:SAAS00462825, ECO:0000313|EMBL:OQX36921.1};
tRNA processing {ECO:0000256|HAMAP-Rule:MF_01849,
ECO:0000256|SAAS:SAAS00721837}.
DOMAIN 111 280 Radical_SAM. {ECO:0000259|Pfam:PF04055}.
REGION 170 171 S-adenosyl-L-methionine binding.
{ECO:0000256|HAMAP-Rule:MF_01849}.
REGION 224 226 S-adenosyl-L-methionine binding.
{ECO:0000256|HAMAP-Rule:MF_01849}.
ACT_SITE 96 96 Proton acceptor. {ECO:0000256|HAMAP-
Rule:MF_01849}.
ACT_SITE 346 346 S-methylcysteine intermediate.
{ECO:0000256|HAMAP-Rule:MF_01849}.
METAL 116 116 Iron-sulfur (4Fe-4S-S-AdoMet).
{ECO:0000256|HAMAP-Rule:MF_01849}.
METAL 120 120 Iron-sulfur (4Fe-4S-S-AdoMet).
{ECO:0000256|HAMAP-Rule:MF_01849}.
METAL 123 123 Iron-sulfur (4Fe-4S-S-AdoMet).
{ECO:0000256|HAMAP-Rule:MF_01849}.
BINDING 202 202 S-adenosyl-L-methionine.
{ECO:0000256|HAMAP-Rule:MF_01849}.
BINDING 303 303 S-adenosyl-L-methionine; via amide
nitrogen and carbonyl oxygen.
{ECO:0000256|HAMAP-Rule:MF_01849}.
DISULFID 109 346 (transient). {ECO:0000256|HAMAP-
Rule:MF_01849}.
SEQUENCE 383 AA; 42305 MW; C7170A30A955EC0B CRC64;
MTAETVKTNL LGLTQSKLEE FFISIGEKKF RAQQVMKWIH HFGVDNFDEM SNVSKALRER
LKACAEIRGP EVVSCDFSAD GTRKWVVRVA SGSCVETVYI PDGKRGTLCV SSQAGCALDC
SFCSTGKQGF NSDLTVAEII GQVWLAAKSF NSVPAKSDRA ITNVVMMGMG EPLLNFNNVM
DSMNLMMDDL GYGLSKRRVT LSTSGVVPAM RRMIGLTDVS LAVSLHAPND VLRNELVPIN
KKYPLKELLA AIHDYMSTLS DKSRVVTIEY TLLKGINDQP EHAEQMIALL RDVPCKINLI
PFNPFPGSGY ECPSNNAIRR FQVMLLEKGF NTTVRKTRGD DIDAACGQLV GQVQDRTRRS
ARYRDQQQQN SGAEPVKVMG MAS


Related products :

Catalog number Product name Quantity
EIAAB44050 Homo sapiens,Human,KIAA1393,M1G-methyltransferase,TRM5,TRMT5,tRNA (guanine-N(1)-)-methyltransferase,tRNA [GM37] methyltransferase,tRNA methyltransferase 5
EIAAB44052 Kiaa1393,M1G-methyltransferase,Mouse,Mus musculus,Trmt5,tRNA (guanine-N(1)-)-methyltransferase,tRNA [GM37] methyltransferase,tRNA methyltransferase 5
EIAAB44051 Bos taurus,Bovine,M1G-methyltransferase,TRMT5,tRNA (guanine-N(1)-)-methyltransferase,tRNA [GM37] methyltransferase,tRNA methyltransferase 5
EIAAB44047 C12orf1,Homo sapiens,Human,Methyltransferase-like protein 1,METTL1,tRNA (guanine-N(7)-)-methyltransferase,tRNA(m7G46)-methyltransferase
EIAAB44045 Bos taurus,Bovine,Methyltransferase-like protein 1,METTL1,tRNA (guanine-N(7)-)-methyltransferase,tRNA(m7G46)-methyltransferase
EIAAB44046 Methyltransferase-like protein 1,Mettl1,Mouse,Mus musculus,tRNA (guanine-N(7)-)-methyltransferase,tRNA(m7G46)-methyltransferase
E2223m Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,Inmt,Mouse,Mus musculus,Temt
E2223Rb Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,INMT,Oryctolagus cuniculus,Rabbit
E2223h Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Homo sapiens,Human,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,INMT
E2223Rb ELISA kit Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,INMT,Oryctolagus cuniculus 96T
U2223Rb CLIA kit Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,INMT,Oryctolagus cuniculus, 96T
E2223m ELISA kit Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,Inmt,Mouse,Mus musculus,Te 96T
U2223m CLIA kit Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,Inmt,Mouse,Mus musculus,Tem 96T
U2223h CLIA kit Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Homo sapiens,Human,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,INMT 96T
E2223h ELISA kit Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Homo sapiens,Human,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,INMT 96T
U2223m CLIA Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,Inmt,Mouse,Mus musculus,Temt 96T
U2223Rb CLIA Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,INMT,Oryctolagus cuniculus,Rabbi 96T
E2223Rb ELISA Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,INMT,Oryctolagus cuniculus,Rabb 96T
E2223m ELISA Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,Inmt,Mouse,Mus musculus,Temt 96T
U2223h CLIA Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Homo sapiens,Human,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,INMT 96T
E2223h ELISA Amine N-methyltransferase,Aromatic alkylamine N-methyltransferase,Arylamine N-methyltransferase,Homo sapiens,Human,Indolamine N-methyltransferase,Indolethylamine N-methyltransferase,INMT 96T
EIAAB44020 D8Ertd812e,Mouse,Mus musculus,N(2),N(2)-dimethylguanosine tRNA methyltransferase,Trmt1,tRNA 2,2-dimethylguanosine-26 methyltransferase,tRNA(guanine-26,N(2)-N(2)) methyltransferase,tRNA(m(2,2)G26)dimet
EIAAB44019 Homo sapiens,Human,N(2),N(2)-dimethylguanosine tRNA methyltransferase,TRMT1,tRNA 2,2-dimethylguanosine-26 methyltransferase,tRNA(guanine-26,N(2)-N(2)) methyltransferase,tRNA(m(2,2)G26)dimethyltransfer
EIAAB26943 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1,18S rRNA Psi1248 methyltransferase,C2F,EMG1,Homo sapiens,Human,Nucleolar protein EMG1 homolog,Protein C2f,Ribosomal RNA small subunit methyltransfer
EIAAB35975 Ftsj3,Protein ftsJ homolog 3,Putative rRNA methyltransferase 3,Rat,Rattus norvegicus,rRNA (uridine-2'-O-)-methyltransferase 3

Kits Elisa; taq POLYMERASE

Search in Google:

google

Share this page:
share on twitter rss feedsfacebookgoogle gentaur



Quick order!
Enter catalog number :