GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Frequency clock protein

 FRQ_NEUCR               Reviewed;         989 AA.
P19970; Q01276; Q7RVB1; V5IKG2; V5IL36;
01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
07-JUN-2005, sequence version 3.
16-JAN-2019, entry version 114.
RecName: Full=Frequency clock protein;
Name=frq; ORFNames=B13D24.170, NCU02265;
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM
1257 / FGSC 987).
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
Sordariomycetes; Sordariomycetidae; Sordariales; Sordariaceae;
Neurospora.
NCBI_TaxID=367110;
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND MUTAGENESIS OF GLU-364; GLY-459;
GLY-482 AND ALA-895.
STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
PubMed=8052643; DOI=10.1073/pnas.91.16.7683;
Aronson B.D., Johnson K.A., Dunlap J.C.;
"Circadian clock locus frequency: protein encoded by a single open
reading frame defines period length and temperature compensation.";
Proc. Natl. Acad. Sci. U.S.A. 91:7683-7687(1994).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
PubMed=12655011; DOI=10.1093/nar/gkg293;
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V.,
Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J.,
Schulte U.;
"What's in the genome of a filamentous fungus? Analysis of the
Neurospora genome sequence.";
Nucleic Acids Res. 31:1944-1954(2003).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
PubMed=12712197; DOI=10.1038/nature01554;
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A.,
Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L.,
Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C.,
Marcotte E., Greenberg D., Roy A., Foley K., Naylor J.,
Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M.,
Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S.,
Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C.,
Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M.,
Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
"The genome sequence of the filamentous fungus Neurospora crassa.";
Nature 422:859-868(2003).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 202-989.
PubMed=2525233; DOI=10.1038/339558a0;
McClung C.R., Fox B.A., Dunlap J.C.;
"The Neurospora clock gene frequency shares a sequence element with
the Drosophila clock gene period.";
Nature 339:558-562(1989).
[5]
PHOSPHORYLATION AT THR-501; SER-513 AND SER-519, AND MUTAGENESIS OF
501-THR--SER-503; 513-SER-THR-514 AND SER-519.
PubMed=10618401; DOI=10.1073/pnas.97.1.234;
Liu Y., Loros J., Dunlap J.C.;
"Phosphorylation of the Neurospora clock protein frequency determines
its degradation rate and strongly influences the period length of the
circadian clock.";
Proc. Natl. Acad. Sci. U.S.A. 97:234-239(2000).
[6]
PHOSPHORYLATION BY CK2.
PubMed=11959847; DOI=10.1101/gad.965102;
Yang Y., Cheng P., Liu Y.;
"Regulation of the Neurospora circadian clock by casein kinase II.";
Genes Dev. 16:994-1006(2002).
-!- FUNCTION: Circadian clock component involved in the generation of
biological rhythms, in particular in rhythm stability, period
length, and temperature compensation. Oscillates in abundance with
a daily peak early in the morning. Behaves as a negative element
in circadian transcriptional loop. May bind to wc-2 protein. The
complex frq-wc-2 may turn off the expression of frq.
-!- INTERACTION:
Q873J5:B9B11.040 (xeno); NbExp=2; IntAct=EBI-6995022, EBI-15874858;
-!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
-!- ALTERNATIVE PRODUCTS:
Event=Alternative initiation; Named isoforms=2;
Name=Long;
IsoId=P19970-1; Sequence=Displayed;
Note=Maintains rhythms at high temperature (30 degrees
Celsius).;
Name=Short;
IsoId=P19970-2; Sequence=VSP_018781;
Note=Maintains rhythms at lower temperature (18 degrees
Celsius).;
-!- INDUCTION: By light; perhaps through white collar-1 (wc-1) and
white collar-2 (wc-2). Also activated directly by wc-1 and wc-2.
-!- PTM: Progressive phosphorylation during the late circadian day and
early night. Phosphorylation is also involved in regulating frq
degradation. Phosphorylation by CKII may have at least three
functions; it decreases the stability of frq, reduces the protein
complex formation between frq and the white collar proteins, and
is important for the closing of the Neurospora circadian negative
feedback loop. {ECO:0000269|PubMed:10618401,
ECO:0000269|PubMed:11959847}.
-!- SIMILARITY: Belongs to the FRQ family. {ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=Ref.4; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; U17073; AAA57121.1; -; mRNA.
EMBL; BX908789; CAF05882.1; -; Genomic_DNA.
EMBL; CM002242; ESA42013.1; -; Genomic_DNA.
EMBL; CM002242; ESA42014.1; -; Genomic_DNA.
EMBL; CM002242; ESA42015.1; -; Genomic_DNA.
PIR; T46659; T46659.
RefSeq; XP_011395124.1; XM_011396822.1. [P19970-2]
RefSeq; XP_011395125.1; XM_011396823.1. [P19970-1]
RefSeq; XP_011395126.1; XM_011396824.1. [P19970-1]
DIP; DIP-41987N; -.
IntAct; P19970; 2.
MINT; P19970; -.
iPTMnet; P19970; -.
PRIDE; P19970; -.
EnsemblFungi; ESA42013; ESA42013; NCU02265. [P19970-1]
EnsemblFungi; ESA42014; ESA42014; NCU02265. [P19970-1]
EnsemblFungi; ESA42015; ESA42015; NCU02265. [P19970-2]
GeneID; 3876095; -.
KEGG; ncr:NCU02265; -.
EuPathDB; FungiDB:NCU02265; -.
InParanoid; P19970; -.
OMA; IYYSGAP; -.
Proteomes; UP000001805; Chromosome 7, Linkage Group VII.
GO; GO:0005737; C:cytoplasm; IEA:InterPro.
GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO; GO:0007623; P:circadian rhythm; IEA:InterPro.
GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
InterPro; IPR018554; FRQ.
Pfam; PF09421; FRQ; 1.
1: Evidence at protein level;
Alternative initiation; Biological rhythms; Complete proteome;
Nucleus; Phosphoprotein; Reference proteome; Transcription;
Transcription regulation.
CHAIN 1 989 Frequency clock protein.
/FTId=PRO_0000021288.
MOTIF 564 568 Nuclear localization signal.
{ECO:0000255}.
COMPBIAS 244 250 Poly-Ser.
COMPBIAS 862 888 Asp/Glu-rich (acidic).
MOD_RES 501 501 Phosphothreonine.
{ECO:0000269|PubMed:10618401}.
MOD_RES 513 513 Phosphoserine.
{ECO:0000269|PubMed:10618401}.
MOD_RES 519 519 Phosphoserine.
{ECO:0000269|PubMed:10618401}.
VAR_SEQ 1 99 Missing (in isoform Short).
{ECO:0000305}.
/FTId=VSP_018781.
MUTAGEN 364 364 E->K: In FRQ3; lengthened period.
{ECO:0000269|PubMed:8052643}.
MUTAGEN 459 459 G->D: In FRQ7; lengthened period.
{ECO:0000269|PubMed:8052643}.
MUTAGEN 482 482 G->S: In FRQ1; shortened period.
{ECO:0000269|PubMed:8052643}.
MUTAGEN 501 503 TPS->APG: In FRQ10; no effect.
{ECO:0000269|PubMed:10618401}.
MUTAGEN 513 514 ST->RA: In FRQ10; strong reduction in the
degradation rate; lengthened period.
{ECO:0000269|PubMed:10618401}.
MUTAGEN 513 513 S->D: In FRQ10; reduces phosphorylation;
slight reduction in the degradation rate;
loss of rhythmicity.
MUTAGEN 513 513 S->I: In FRQ10; reduces phosphorylation;
strong reduction in the degradation rate;
lengthened period.
MUTAGEN 519 519 S->A: In FRQ10; no effect.
{ECO:0000269|PubMed:10618401}.
MUTAGEN 663 989 Missing: In FRQ9; loss of rhythmicity.
MUTAGEN 895 895 A->T: In FRQ2; shortened period.
{ECO:0000269|PubMed:8052643}.
CONFLICT 146 146 S -> T (in Ref. 1; AAA57121).
{ECO:0000305}.
CONFLICT 202 207 EATLRD -> MGGNRP (in Ref. 4).
{ECO:0000305}.
CONFLICT 207 207 D -> H (in Ref. 1; AAA57121).
{ECO:0000305}.
CONFLICT 233 233 H -> Y (in Ref. 1; AAA57121).
{ECO:0000305}.
CONFLICT 962 963 ML -> IV (in Ref. 1; AAA57121 and 4).
{ECO:0000305}.
SEQUENCE 989 AA; 108200 MW; EC4E984FAD39F095 CRC64;
MADSGDKSQG MRPPPFDSRG HPLPRRASPD KSITLENHRL ARDTSSRVTS SSALGVTESQ
PQLKSSPTRR NSSGESEPTN WFNQSNRNPA AAFHDESHIM EVDPPFYQKE TDSSNEESRY
PPGRNPVHPP GGVQLPGFRP VAAHSSAADD YRSVIDDLTV ENKRLKEELK RYKQFGSDVM
RKEKLFEIKV HGLPRRKKRE LEATLRDFAA SLGDSSESTS QRRKTGRHGT AVHSSGVSLS
KHDSSSSSRS RPVDSAYNSM STGRSSHAPH SSGPSLGRPS LTRAKSVGTQ KVENYLRDTP
DGLLPHHIVM TDKEKKKLVV RRLEQLFTGK ISGRNMQRNQ SMPSMDAPLA PEGTNMAPPR
PPPEGLREAC IQLQDGDNPR KNRSSKDNGS ASNSGGDQTE LGGTGTGSGD GSGSGGRTGN
NTSPPGAIAP DQRPTRPRDL DPDRVQIPSE NMDYIRHLGL VSPEFLQGSR TSYQDVAPDA
EGWVYLNLLC NLAQLHMVNV TPSFIRQAVS EKSTKFQLSA DGRKIRWRGG TDGTKFSSDS
SEDKSQQSPM TEDTEDGSDK NGRRKKRKTQ QASSEIGRFG PSRSPSDTFH YKPMFVHRNS
SSIETSLEES MSQGSEDAVD ESNMGNSKWD FSGSGTTQQR RKRRYDGAIV YYTGAPFCTD
LSGDPGDMSP TAQMTAGREV EGSGSGDEVE HVLQRTLSGS SLPIRPLSDD RARVAEVLDF
DPGNPPELVA DDGSSPNDED FVFPWCEDPA KVRIQPIAKE VMEPSGLGGV LPDDHFVMLV
TTRRVVRPIL QRQLSRSTTS EDTAEFIAER LAAIRTSSPL PPRSHRLTVA PLQVEYVSGQ
FRRLNPAPLP PPAIFYPPFS TDSSWDDGDD LASDDEEVEE VEEDSYSEGQ ISRRANPHFS
DNNTYMRKDD LAFDTETDVR MDSDDNRLSD SGHNMRAMMP RAEAVDGDDS PLAAVTGKEV
DMLHTGSSVA TAGGAESGYS SSMEDVSSS


Related products :

Catalog number Product name Quantity
DL-CLOCK-Hu Human Clock Homolog (CLOCK) ELISA Kit 96T
20-372-60193 clock homolog (mouse) (CLOCK) - Mouse monoclonal anti-human CLOCK antibody; hCLOCK Monoclonal 0.1 mg
E84116Hu ELISA Kit for Clock Homolog (CLOCK) Organism: Homo sapiens (Human) 96T
SEQ116Hu ELISA Kit for Clock Homolog (CLOCK) Homo sapiens (Human) 96T
CSB-EL005574RA Rat clock homolog (mouse) (CLOCK) ELISA kit, Species Rat, Sample Type serum, plasma 96T
60B171a Polyclonal Antibodies: CLOCK; Specificity: CLOCK ; Application: IHC 0.1mg
E84116Hu ELISA Kit for Clock Homolog (CLOCK), Organism Homo sapiens (Human), Detection range 0.312-20ng_mL 96T/Kit
E2900Hu Human Clock Homolog (CLOCK)ELISA Kit 48T
201-12-2901 Human Clock Homolog(CLOCK)ELISA Kit 96T
201-12-2901 Human Clock Homolog (CLOCK)ELISA Kit 96T
201-12-2901 Human Clock Homolog (CLOCK)ELISA Kit 48T
E2900Hu Human Clock Homolog (CLOCK)ELISA Kit 96T
3896BP-50 CLOCK Blocking Peptide target: CLOCK 50 μg
CLPB CLOCK Gene clock homolog (mouse)
YHB0741Hu Human Clock Homolog (CLOCK)ELISA Kit 48T
UT-E05041 Human Clock Homolog (CLOCK) ELISA Kit 96T
GWB-97038D clock homolog (mouse) (CLOCK), Antibody
YHB0741Hu Human Clock Homolog (CLOCK)ELISA Kit 96T
QY-E05041 Human Clock Homolog(CLOCK)ELISA Kit 96T
UB-E05041 Human Clock Homolog(CLOCK)ELISA Kit 96T
EIAAB10416 CCDC88C,Coiled-coil domain-containing protein 88C,DAPLE,Dvl-associating protein with a high frequency of leucine residues,hDaple,HkRP2,Homo sapiens,Hook-related protein 2,Human,KIAA1509,Protein Daple
EIAAB10417 Ccdc88c,Coiled-coil domain-containing protein 88C,Daple,Dvl-associating protein with a high frequency of leucine residues,Mouse,Mus musculus,Protein Daple
E84116Hu ELISA Kit for Clock Homolog (CLOCK) 96T/Kit
3896BP-50 CLOCK Blocking Peptide CLOCK 50 μg
60193 IgG,clock homolog (mouse) (CLOCK) 0.1 mg
Pathways :
WP2324: AGE/RAGE pathway
WP20: Cytoplasmic Ribosomal Proteins
WP1657: Glycerolipid metabolism
WP2125: Circadian clock tutorial_eliene
WP1187: Cytoplasmic Ribosomal Proteins
WP1268: Diurnally regulated genes with circadian orthologs
WP1700: Selenoamino acid metabolism
WP1294: NLR proteins
WP1379: Diurnally regulated genes with circadian orthologs
WP1797: Circadian Clock
WP35: G Protein Signaling Pathways
WP324: Cytoplasmic Ribosomal Proteins
WP1663: Homologous recombination
WP2133: Circadian clock tutorial_Max
WP544: Circadian Exercise
WP799: NLR proteins
WP1673: Naphthalene and anthracene degradation
WP2138: Circadian clock tutorial_i6003923
WP1780: ABC-family proteins mediated transport
WP1502: Mitochondrial biogenesis
WP1909: Signal regulatory protein (SIRP) family interactions
WP1834: Interactions of the immunoglobulin superfamily (IgSF) member proteins
WP1613: 1,4-Dichlorobenzene degradation
WP2032: TSH signaling pathway
WP731: Sterol regulatory element binding protein related

Related Genes :
[frq B13D24.170 NCU02265] Frequency clock protein
[Per1 Per Rigui] Period circadian protein homolog 1 (mPER1) (Circadian clock protein PERIOD 1) (Circadian pacemaker protein Rigui)
[Per2] Period circadian protein homolog 2 (mPER2) (Circadian clock protein PERIOD 2)
[PER1 KIAA0482 PER RIGUI] Period circadian protein homolog 1 (hPER1) (Circadian clock protein PERIOD 1) (Circadian pacemaker protein Rigui)
[CLOCK BHLHE8 KIAA0334] Circadian locomoter output cycles protein kaput (hCLOCK) (EC 2.3.1.48) (Class E basic helix-loop-helix protein 8) (bHLHe8)
[ADO1 FKL2 LKP1 ZTL At5g57360 MSF19.2] Adagio protein 1 (Clock-associated PAS protein ZTL) (F-box only protein 2b) (FBX2b) (Flavin-binding kelch repeat F-box protein 1-like protein 2) (FKF1-like protein 2) (LOV kelch protein 1) (Protein ZEITLUPE)
[PER2 KIAA0347] Period circadian protein homolog 2 (hPER2) (Circadian clock protein PERIOD 2)
[Per2] Period circadian protein homolog 2 (rPER2) (Circadian clock protein PERIOD 2)
[PER3 GIG13] Period circadian protein homolog 3 (hPER3) (Cell growth-inhibiting gene 13 protein) (Circadian clock protein PERIOD 3)
[Clock] Circadian locomoter output cycles protein kaput (mCLOCK) (EC 2.3.1.48)
[Per3] Period circadian protein homolog 3 (mPER3) (Circadian clock protein PERIOD 3)
[Per1] Period circadian protein homolog 1 (rPER1) (Circadian clock protein PERIOD 1)
[CCA1 At2g46830 F19D11] Protein CCA1 (MYB-related transcription factor CCA1) (Protein CIRCADIAN CLOCK ASSOCIATED 1)
[Clock] Circadian locomoter output cycles protein kaput (EC 2.3.1.48)
[Clock] Circadian locomoter output cycles protein kaput (rCLOCK) (EC 2.3.1.48)
[Clock] Circadian locomoter output cycles protein kaput (EC 2.3.1.48)
[CLOCK] Circadian locomoter output cycles protein kaput (EC 2.3.1.48)
[per CG2647] Period circadian protein (Protein clock-6) (CLK-6)
[PASD1] Circadian clock protein PASD1 (Cancer/testis antigen 63) (CT63) (OX-TES-1) (PAS domain-containing protein 1)
[kaiA Synpcc7942_1218 see0009] Circadian clock protein KaiA
[kaiC Synpcc7942_1216 see0011] Circadian clock protein kinase KaiC (EC 2.7.11.1)
[CLOCK] Circadian locomoter output cycles protein kaput
[Clk CLOCK jrk PAS1 CG7391] Circadian locomoter output cycles protein kaput (dCLOCK) (dPAS1)
[Clock] Circadian locomoter output cycles protein kaput (EC 2.3.1.48)
[Cipc Kiaa1737] CLOCK-interacting pacemaker (CLOCK-interacting circadian protein)
[PER2] Period circadian protein homolog 2 (sPER2) (Circadian clock protein PERIOD 2)
[LNK1 At5g64170 MHJ24.1] Protein LNK1 (Night light-inducible and clock-regulated 1)
[kaiC tlr0483] Circadian clock protein kinase KaiC (EC 2.7.11.1)
[LNK2 At3g54500 T14E10.70] Protein LNK2 (Night light-inducible and clock-regulated 2)
[kaiB Synpcc7942_1217 see0010] Circadian clock protein KaiB

Bibliography :
[31374999] Molecular Evolution of Classic Human Astrovirus, as Revealed by the Analysis of the Capsid Protein Gene.
[31344120] The circadian rhythm of bladder clock genes in the spontaneously hypersensitive rat.
[31248072] An Inactivation Switch Enables Rhythms in a Clock Model.
[31171626] Multiplex profiling of peritoneal metastases from gastric adenocarcinoma identified novel targets and molecular subtypes that predict treatment response.
[30954403] The Phospho-Code Determining Circadian Feedback Loop Closure and Output in Neurospora.
[30931960] A decade of sustained selection pressure on two surface sites of the VP1 protein of Enterovirus A71 suggests that immune evasion may be an indirect driver for virulence.
[30926261] Segmentation clock dynamics is strongly synchronized in the forming somite.
[30708173] Bayesian phylogenetic analysis of the influenza-A virus genomes isolated in Tunisia, and determination of potential recombination events.
[30662675] Circadian protein CLK suppresses transforming growth factor-β expression in peripheral B cells of nurses with day-night shift rotation.