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HPr kinase/phosphorylase (HPrK/P) (EC 2.7.11.-) (EC 2.7.4.-) (HPr(Ser) kinase/phosphorylase)

 A0A1U7ECI5_9LACT        Unreviewed;       312 AA.
A0A1U7ECI5;
10-MAY-2017, integrated into UniProtKB/TrEMBL.
10-MAY-2017, sequence version 1.
16-JAN-2019, entry version 10.
RecName: Full=HPr kinase/phosphorylase {ECO:0000256|HAMAP-Rule:MF_01249, ECO:0000256|SAAS:SAAS00754002};
Short=HPrK/P {ECO:0000256|HAMAP-Rule:MF_01249};
EC=2.7.11.- {ECO:0000256|HAMAP-Rule:MF_01249, ECO:0000256|SAAS:SAAS00754007};
EC=2.7.4.- {ECO:0000256|HAMAP-Rule:MF_01249, ECO:0000256|SAAS:SAAS00754021};
AltName: Full=HPr(Ser) kinase/phosphorylase {ECO:0000256|HAMAP-Rule:MF_01249};
Name=hprK {ECO:0000256|HAMAP-Rule:MF_01249};
ORFNames=BW721_06825 {ECO:0000313|EMBL:APZ49416.1};
Jeotgalibaca sp. PTS2502.
Bacteria; Firmicutes; Bacilli; Lactobacillales; Carnobacteriaceae;
Jeotgalibaca.
NCBI_TaxID=1903686 {ECO:0000313|EMBL:APZ49416.1, ECO:0000313|Proteomes:UP000187137};
[1] {ECO:0000313|EMBL:APZ49416.1, ECO:0000313|Proteomes:UP000187137}
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=PTS2502 {ECO:0000313|EMBL:APZ49416.1,
ECO:0000313|Proteomes:UP000187137};
Mah S.A., Swanson W.J., Moy G.W., Vacquier V.D.;
Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
-!- FUNCTION: Catalyzes the ATP- as well as the pyrophosphate-
dependent phosphorylation of a specific serine residue in HPr, a
phosphocarrier protein of the phosphoenolpyruvate-dependent sugar
phosphotransferase system (PTS). HprK/P also catalyzes the
pyrophosphate-producing, inorganic phosphate-dependent
dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-
Ser-HPr). The two antagonistic activities of HprK/P are regulated
by several intracellular metabolites, which change their
concentration in response to the absence or presence of rapidly
metabolisable carbon sources (glucose, fructose, etc.) in the
growth medium. Therefore, by controlling the phosphorylation state
of HPr, HPrK/P is a sensor enzyme that plays a major role in the
regulation of carbon metabolism and sugar transport: it mediates
carbon catabolite repression (CCR), and regulates PTS-catalyzed
carbohydrate uptake and inducer exclusion. {ECO:0000256|HAMAP-
Rule:MF_01249}.
-!- CATALYTIC ACTIVITY:
Reaction=[HPr protein]-L-serine + ATP = [HPr protein]-O-phospho-L-
serine + ADP + H(+); Xref=Rhea:RHEA:46600, Rhea:RHEA-COMP:11602,
Rhea:RHEA-COMP:11603, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999,
ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216;
Evidence={ECO:0000256|HAMAP-Rule:MF_01249,
ECO:0000256|SAAS:SAAS01117191};
-!- CATALYTIC ACTIVITY:
Reaction=[HPr protein]-O-phospho-L-serine + H(+) + phosphate =
[HPr protein]-L-serine + diphosphate; Xref=Rhea:RHEA:46604,
Rhea:RHEA-COMP:11602, Rhea:RHEA-COMP:11603, ChEBI:CHEBI:15378,
ChEBI:CHEBI:29999, ChEBI:CHEBI:33019, ChEBI:CHEBI:43474,
ChEBI:CHEBI:83421; Evidence={ECO:0000256|HAMAP-Rule:MF_01249,
ECO:0000256|SAAS:SAAS01117182};
-!- COFACTOR:
Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Evidence={ECO:0000256|HAMAP-Rule:MF_01249,
ECO:0000256|SAAS:SAAS00754019};
-!- SUBUNIT: Homohexamer. {ECO:0000256|HAMAP-Rule:MF_01249,
ECO:0000256|SAAS:SAAS00754027}.
-!- DOMAIN: The Walker A ATP-binding motif also binds Pi and PPi.
{ECO:0000256|HAMAP-Rule:MF_01249}.
-!- MISCELLANEOUS: Both phosphorylation and phosphorolysis are carried
out by the same active site and suggest a common mechanism for
both reactions. {ECO:0000256|HAMAP-Rule:MF_01249}.
-!- SIMILARITY: Belongs to the HPrK/P family. {ECO:0000256|HAMAP-
Rule:MF_01249, ECO:0000256|SAAS:SAAS00754005}.
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EMBL; CP019433; APZ49416.1; -; Genomic_DNA.
RefSeq; WP_076767360.1; NZ_CP019433.1.
KEGG; jep:BW721_06825; -.
KO; K06023; -.
OrthoDB; 391150at2; -.
Proteomes; UP000187137; Chromosome.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
GO; GO:0004712; F:protein serine/threonine/tyrosine kinase activity; IEA:UniProtKB-UniRule.
GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
GO; GO:0006109; P:regulation of carbohydrate metabolic process; IEA:UniProtKB-UniRule.
CDD; cd01918; HprK_C; 1.
Gene3D; 3.40.1390.20; -; 1.
HAMAP; MF_01249; HPr_kinase; 1.
InterPro; IPR003755; HPr(Ser)_kin/Pase.
InterPro; IPR011104; Hpr_kin/Pase_C.
InterPro; IPR011126; Hpr_kin/Pase_Hpr_N.
InterPro; IPR028979; Ser_kin/Pase_Hpr-like_N_sf.
PANTHER; PTHR30305:SF1; PTHR30305:SF1; 1.
Pfam; PF07475; Hpr_kinase_C; 1.
Pfam; PF02603; Hpr_kinase_N; 1.
SUPFAM; SSF75138; SSF75138; 1.
TIGRFAMs; TIGR00679; hpr-ser; 1.
3: Inferred from homology;
ATP-binding {ECO:0000256|HAMAP-Rule:MF_01249,
ECO:0000256|SAAS:SAAS00754014};
Carbohydrate metabolism {ECO:0000256|HAMAP-Rule:MF_01249};
Complete proteome {ECO:0000313|Proteomes:UP000187137};
Kinase {ECO:0000256|HAMAP-Rule:MF_01249,
ECO:0000256|SAAS:SAAS00754023};
Magnesium {ECO:0000256|HAMAP-Rule:MF_01249,
ECO:0000256|SAAS:SAAS00754024};
Metal-binding {ECO:0000256|HAMAP-Rule:MF_01249,
ECO:0000256|SAAS:SAAS00754029};
Multifunctional enzyme {ECO:0000256|HAMAP-Rule:MF_01249,
ECO:0000256|SAAS:SAAS00754025};
Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01249,
ECO:0000256|SAAS:SAAS00754014};
Reference proteome {ECO:0000313|Proteomes:UP000187137};
Serine/threonine-protein kinase {ECO:0000256|HAMAP-Rule:MF_01249,
ECO:0000256|SAAS:SAAS00754023};
Transferase {ECO:0000256|HAMAP-Rule:MF_01249,
ECO:0000256|SAAS:SAAS00754023}.
DOMAIN 5 128 Hpr_kinase_N. {ECO:0000259|Pfam:PF02603}.
DOMAIN 131 299 Hpr_kinase_C. {ECO:0000259|Pfam:PF07475}.
NP_BIND 154 161 ATP. {ECO:0000256|HAMAP-Rule:MF_01249}.
REGION 202 211 Important for the catalytic mechanism of
both phosphorylation and
dephosphorylation. {ECO:0000256|HAMAP-
Rule:MF_01249}.
REGION 265 270 Important for the catalytic mechanism of
dephosphorylation. {ECO:0000256|HAMAP-
Rule:MF_01249}.
ACT_SITE 139 139 {ECO:0000256|HAMAP-Rule:MF_01249}.
ACT_SITE 160 160 {ECO:0000256|HAMAP-Rule:MF_01249}.
ACT_SITE 178 178 Proton acceptor; for phosphorylation
activity. Proton donor; for
dephosphorylation activity.
{ECO:0000256|HAMAP-Rule:MF_01249}.
ACT_SITE 244 244 {ECO:0000256|HAMAP-Rule:MF_01249}.
METAL 161 161 Magnesium. {ECO:0000256|HAMAP-
Rule:MF_01249}.
METAL 203 203 Magnesium. {ECO:0000256|HAMAP-
Rule:MF_01249}.
SEQUENCE 312 AA; 35112 MW; C70030466C285BDB CRC64;
MSNSVTIQEL VKTSTFKIIV GEEFLNKKIT STEIYRPGVE LTGYFAFYPS WRVQLMGQTE
LSFIERMTPE ERLVIMRRLC QKETPCFIIS RNMEAPFELV KACQEAGIPL LQAQSKTTRV
SSNVTNFLES RLAERVSMHG VLVDVFGMGV MITGDSGVGK SETALELIQK GHRLVADDRI
DLYQHDEDTL MGESPPILRH LIEIRGIGIM DVMTLFGAGA VKQSNEVNLI VNLSLWAKDK
KFERLGSTEE MVNILDVQVP KITVPVKTGR NLAIIVEMAA MNFRAKTMGY NAAETFERNL
DLLIKENSKE ED


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Pathways :
WP1493: Carbon assimilation C4 pathway
WP1567: Glycolysis and Gluconeogenesis
WP1619: Amino sugar and nucleotide sugar metabolism
WP1653: Galactose metabolism
WP1681: Pantothenate and CoA biosynthesis
WP1701: Starch and sucrose metabolism
WP1703: Streptomycin biosynthesis
WP1844: MAP kinase cascade
WP1946: Cori Cycle
WP2340: Thiamine (vitamin B1) biosynthesis and salvage
WP2341: vitamin B1 (thiamin) biosynthesis and salvage pathway
WP253: Glycolysis
WP32: Translation Factors

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Bibliography :
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