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Matrix protein VP40 (Ebola VP40) (eVP40) (Membrane-associated protein VP40)

 VP40_EBOZ5              Reviewed;         326 AA.
Q77DJ6;
11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
05-JUL-2004, sequence version 1.
05-JUN-2019, entry version 83.
RecName: Full=Matrix protein VP40;
AltName: Full=Ebola VP40;
Short=eVP40;
AltName: Full=Membrane-associated protein VP40;
Name=VP40;
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus).
Viruses; Riboviria; Negarnaviricota; Haploviricotina; Monjiviricetes;
Mononegavirales; Filoviridae; Ebolavirus.
NCBI_TaxID=128951;
NCBI_TaxID=77231; Epomops franqueti (Franquet's epauleted fruit bat).
NCBI_TaxID=9606; Homo sapiens (Human).
NCBI_TaxID=77243; Myonycteris torquata (Little collared fruit bat).
[1]
NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Chain P.S.G., Ichou M.A., Malfatti S.A., Hajjaj A., Vergez L.M.,
Paragas J., Do L.H., Jahrling P.B., Smith K.L., McCready P.M.,
Ibrahim M.S.;
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
[2]
X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 31-326.
PubMed=10944105; DOI=10.1093/emboj/19.16.4228;
Dessen A., Volchkov V., Dolnik O., Klenk H.-D., Weissenhorn W.;
"Crystal structure of the matrix protein VP40 from Ebola virus.";
EMBO J. 19:4228-4236(2000).
-!- FUNCTION: Plays an essential role virus particle assembly and
budding. Acts by interacting with viral ribonucleocapsid and host
members of the ESCRT (endosomal sorting complex required for
transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or
TGS101. May play a role in immune cell dysfunction by being
packaged into exosomes that can decrease the viability of
recipient cells. {ECO:0000250|UniProtKB:Q05128}.
-!- SUBUNIT: Exists as a dimer until it reorganizes at the plasma
membrane into a hexameric form using phosphatidylinositol 4,5-
bisphosphate (PI(4,5)P2). Interacts with host TSG101. Homo-hexamer
interacts with the WW domain 3 of host NEDD4. Interacts with the
nucleoprotein/NP. Interacts (via YPx(n)L/I motif) with host
PDCD6IP/ALIX; this interaction supports efficient egress of viral
particles. Interacts with VP35. Interacts with host ITCH; this
interaction is required for efficient egress.
{ECO:0000250|UniProtKB:Q05128}.
-!- SUBCELLULAR LOCATION: Host cytoplasm
{ECO:0000250|UniProtKB:Q05128}. Host cell membrane
{ECO:0000250|UniProtKB:Q05128}. Virion membrane
{ECO:0000250|UniProtKB:P35260}; Peripheral membrane protein
{ECO:0000250|UniProtKB:P35260}. Host late endosome membrane
{ECO:0000250|UniProtKB:P35260}; Peripheral membrane protein
{ECO:0000250|UniProtKB:P35260}. Host cell membrane
{ECO:0000250|UniProtKB:P35260}; Peripheral membrane protein
{ECO:0000250|UniProtKB:P35260}; Cytoplasmic side
{ECO:0000250|UniProtKB:P35260}. Host endomembrane system
{ECO:0000250|UniProtKB:P35260}; Peripheral membrane protein
{ECO:0000250|UniProtKB:P35260}. Secreted, exosome
{ECO:0000250|UniProtKB:Q05128}. Note=In virion, localizes on the
inner side of the membrane. In the host cell, it is found
associated with virus-induced membrane proliferation foci and
probably also in multivesicular bodies. These VP40-enriched
membrane clusters are then redistributed to the plasma membrane
where budding takes place. {ECO:0000250|UniProtKB:P35260}.
-!- DOMAIN: Late-budding domains (L domains) are short sequence motifs
essential for viral particle budding. They recruit proteins of the
host ESCRT machinery (Endosomal Sorting Complex Required for
Transport) or ESCRT-associated proteins. VP40 contains two
overlapping L domains: a PTAP/PSAP motif, which interacts with the
UEV domain of TSG101 and a PPXY motif which interacts with the WW
domain 3 of NEDD4. {ECO:0000250|UniProtKB:Q05128}.
-!- PTM: Sumoylated with host SUMO1, SUMO2. Sumoylation provides
stability to VP40. {ECO:0000250|UniProtKB:Q05128}.
-!- PTM: Ubiquitinated by host WWP1. This modification mediates
efficient viral budding. {ECO:0000250|UniProtKB:Q05128}.
-!- MISCELLANEOUS: Most abundant protein in the virion.
{ECO:0000250|UniProtKB:Q05128}.
-!- SIMILARITY: Belongs to the filoviridae matrix protein VP40 family.
{ECO:0000305}.
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EMBL; AY354458; AAQ55047.1; -; Genomic_RNA.
RefSeq; NP_066245.1; NC_002549.1.
PDB; 1ES6; X-ray; 2.00 A; A=31-326.
PDBsum; 1ES6; -.
SMR; Q77DJ6; -.
PRIDE; Q77DJ6; -.
GeneID; 911825; -.
KEGG; vg:911825; -.
EvolutionaryTrace; Q77DJ6; -.
Proteomes; UP000007208; Genome.
GO; GO:0070062; C:extracellular exosome; IEA:UniProtKB-SubCell.
GO; GO:0033645; C:host cell endomembrane system; IEA:UniProtKB-SubCell.
GO; GO:0044185; C:host cell late endosome membrane; IEA:UniProtKB-SubCell.
GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
GO; GO:0039660; F:structural constituent of virion; IEA:UniProtKB-KW.
GO; GO:0039702; P:viral budding via host ESCRT complex; IEA:UniProtKB-KW.
Gene3D; 2.60.510.10; -; 1.
InterPro; IPR008986; EV_matrix.
InterPro; IPR035092; EV_matrix_protein_C-terminal.
InterPro; IPR038057; EV_matrix_sf.
Pfam; PF07447; VP40; 1.
PIRSF; PIRSF018327; VP40_FiloV; 1.
SUPFAM; SSF50012; SSF50012; 2.
1: Evidence at protein level;
3D-structure; Complete proteome; Host cell membrane; Host cytoplasm;
Host endosome; Host membrane; Host-virus interaction; Isopeptide bond;
Membrane; Ribonucleoprotein; RNA-binding; Secreted; Ubl conjugation;
Viral budding; Viral budding via the host ESCRT complexes;
Viral matrix protein; Viral release from host cell;
Viral RNA replication; Virion.
CHAIN 1 326 Matrix protein VP40.
/FTId=PRO_0000245083.
REGION 212 214 Important for oligomerization.
{ECO:0000250}.
REGION 213 326 Membrane-binding.
MOTIF 7 10 PTAP/PSAP motif.
MOTIF 10 13 PPXY motif.
HELIX 61 63 {ECO:0000244|PDB:1ES6}.
STRAND 71 83 {ECO:0000244|PDB:1ES6}.
STRAND 86 101 {ECO:0000244|PDB:1ES6}.
STRAND 103 106 {ECO:0000244|PDB:1ES6}.
HELIX 108 116 {ECO:0000244|PDB:1ES6}.
STRAND 120 125 {ECO:0000244|PDB:1ES6}.
STRAND 128 130 {ECO:0000244|PDB:1ES6}.
STRAND 132 137 {ECO:0000244|PDB:1ES6}.
HELIX 148 152 {ECO:0000244|PDB:1ES6}.
STRAND 153 158 {ECO:0000244|PDB:1ES6}.
HELIX 159 162 {ECO:0000244|PDB:1ES6}.
STRAND 173 185 {ECO:0000244|PDB:1ES6}.
STRAND 203 208 {ECO:0000244|PDB:1ES6}.
HELIX 228 231 {ECO:0000244|PDB:1ES6}.
HELIX 234 243 {ECO:0000244|PDB:1ES6}.
HELIX 244 246 {ECO:0000244|PDB:1ES6}.
STRAND 248 253 {ECO:0000244|PDB:1ES6}.
HELIX 254 256 {ECO:0000244|PDB:1ES6}.
STRAND 258 262 {ECO:0000244|PDB:1ES6}.
HELIX 265 271 {ECO:0000244|PDB:1ES6}.
STRAND 284 288 {ECO:0000244|PDB:1ES6}.
TURN 298 301 {ECO:0000244|PDB:1ES6}.
STRAND 304 310 {ECO:0000244|PDB:1ES6}.
SEQUENCE 326 AA; 35183 MW; 1AB132C0DB8E9003 CRC64;
MRRVILPTAP PEYMEAIYPV RSNSTIARGG NSNTGFLTPE SVNGDTPSNP LRPIADDTID
HASHTPGSVS SAFILEAMVN VISGPKVLMK QIPIWLPLGV ADQKTYSFDS TTAAIMLASY
TITHFGKATN PLVRVNRLGP GIPDHPLRLL RIGNQAFLQE FVLPPVQLPQ YFTFDLTALK
LITQPLPAAT WTDDTPTGSN GALRPGISFH PKLRPILLPN KSGKKGNSAD LTSPEKIQAI
MTSLQDFKIV PIDPTKNIMG IEVPETLVHK LTGKKVTSKN GQPIIPVLLP KYIGLDPVAP
GDLTMVITQD CDTCHSPASL PAVIEK


Related products :

Catalog number Product name Quantity
0501-020 Recombinant ZEBOV VP40 protein, Zaire ebolavirus 100 ug
0501-020 Recombinant ZEBOV VP40
0303-001 Rabbit anti-(MARV VP40) pAb
0301-010 Rabbit anti-(ZEBOV VP40) pAb
0203-012 Mouse anti-(MARV VP40) mAb (1D1)
0303-001 Rabbit anti-(MARV VP40) polyclonal 100 ug
0303-001 Rabbit anti-(MARV VP40) polyclonal
0301-010 Rabbit anti-(ZEBOV VP40) polyclonal
0301-010 Rabbit anti-(ZEBOV VP40) polyclonal 100 ug
0202-018 Mouse anti-(SEBOV VP40) mAb (1G10)
0203-012 Mouse anti-(MARV VP40) mAb (1D1), Monoclonal antibody 100 ug
0301-010 Rabbit anti-(ZEBOV VP40) pAb, Polyclonal antibody 100 ug
0303-001 Rabbit anti-(MARV VP40) pAb, Polyclonal antibody 100 ug
0202-018 Mouse anti-SEBOV VP40 mAb (1G10), Monoclonal antibody 100 ug
OBT1907X RECOMBINANT SARS MATRIX PROTEIN (aa182_216), Product Type Recombinant Protein, Specificity SARS MEMBRANE PROTEIN , Target Species Viral, Host N_A, Format Rec. Protein, Isotypes , Applications E 1 mg
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EIAAB07951 Acam,Adipocyte adhesion molecule,Adipocyte-specific protein 5,Asam,Asp5,CAR-like membrane protein,Clmp,Coxsackie- and adenovirus receptor-like membrane protein,CXADR-like membrane protein,Mouse,Mus mu
EIAAB07950 Acam,Adipocyte adhesion molecule,Asam,CAR-like membrane protein,Clmp,Coxsackie- and adenovirus receptor-like membrane protein,CXADR-like membrane protein,Rat,Rattus norvegicus
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orb81536 SARS Associated Coronavirus Matrix protein The E.coli derived 30kDa recombinant protein contains the Matrix protein 182-216 amino acids immunodominant regions. For research use only. 100
E1639b ELISA 22 kDa extracellular matrix protein,Bos taurus,Bovine,Dermatan sulfate proteoglycan-associated protein 22K,Dermatopontin,DPT,TRAMP,Tyrosine-rich acidic matrix protein 96T
Pathways :
WP1692: Protein export
WP2199: Seed Development
WP1665: Limonene and pinene degradation
WP1714: Tyrosine metabolism
WP1689: Porphyrin and chlorophyll metabolism
WP1438: Influenza A virus infection
WP1502: Mitochondrial biogenesis
WP1713: Two-component system
WP1624: Bacterial secretion system
WP1654: gamma-Hexachlorocyclohexane degradation
WP2203: TSLP Signaling Pathway
WP1371: G Protein Signaling Pathways
WP931: G Protein Signaling Pathways
WP1493: Carbon assimilation C4 pathway
WP1700: Selenoamino acid metabolism
WP1661: Glyoxylate and dicarboxylate metabolism
WP2292: Chemokine signaling pathway
WP1672: Mismatch repair
WP2371: Parkinsons Disease Pathway
WP1566: Citrate cycle (TCA cycle)
WP1616: ABC transporters
WP1892: Protein folding
WP1676: Non-homologous end-joining
WP35: G Protein Signaling Pathways
WP1685: Peptidoglycan biosynthesis

Related Genes :
[VP40] Matrix protein VP40 (Ebola VP40) (eVP40) (Membrane-associated protein VP40)
[VP40] Matrix protein VP40 (Ebola VP40) (eVP40) (Membrane-associated protein VP40)
[VP40] Matrix protein VP40 (Ebola VP40) (eVP40) (Membrane-associated protein VP40)
[VP40] Matrix protein VP40 (Marburg VP40) (mVP40) (Membrane-associated protein VP40)
[] RNA2 polyprotein (Genome polyprotein M) (M RNA polyprotein) (Middle component RNA polyprotein) (P2) [Cleaved into: VP58; Movement protein (MP) (43 kDa protein); Large capsid protein (LCP) (Coat protein VP40) (L subunit) (Large coat protein); Small capsid protein precursor (S subunit); Mature small capsid protein (SCP) (Coat protein VP22) (Small capsid protein, N-terminus part) (Small coat protein, N-terminus part); Small capsid protein C-terminus part (Small coat protein C-terminus part)]
[VP40] Matrix protein VP40 (Membrane-associated protein VP40)
[VP35] Polymerase cofactor VP35 (Ebola VP35) (eVP35)
[VP40] Matrix protein VP40 (Membrane-associated protein VP40)
[VP40] Matrix protein VP40 (Membrane-associated protein VP40)
[VP40] Matrix protein VP40 (Marburg VP40) (mVP40) (Membrane-associated protein VP40)
[VP40] Matrix protein VP40 (Marburg VP40) (mVP40) (Membrane-associated protein VP40)
[VP40] Matrix protein VP40 (Marburg VP40) (mVP40) (Membrane-associated protein VP40)
[VP40] Matrix protein VP40 (Marburg VP40) (mVP40) (Membrane-associated protein VP40)
[GP] Envelope glycoprotein (GP1,2) (GP) [Cleaved into: Shed GP (GP1,2-delta); GP1; GP2]
[GP] Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
[TSG101] Tumor susceptibility gene 101 protein (ESCRT-I complex subunit TSG101)
[ISG15 G1P2 UCRP] Ubiquitin-like protein ISG15 (Interferon-induced 15 kDa protein) (Interferon-induced 17 kDa protein) (IP17) (Ubiquitin cross-reactive protein) (hUCRP)
[ITCH] E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1)
[IQGAP1 KIAA0051] Ras GTPase-activating-like protein IQGAP1 (p195)
[VP40 vp40] Matrix protein VP40 (Membrane-associated protein VP40)
[VP40 DF49_50518gpVP40 ZEBOVgp3] Matrix protein VP40 (Membrane-associated protein VP40)
[UL26] Capsid scaffolding protein (Capsid protein P40) (Protease precursor) (pPR) (Virion structural protein UL26) [Cleaved into: Assemblin (EC 3.4.21.97) (Protease); Assembly protein (Capsid assembly protein)]
[BVRF2] Capsid scaffolding protein (Capsid protein P40) (Protease precursor) (pPR) (Protein EC-RF3/EC-RF3A) (Virion structural protein BVRF2) [Cleaved into: Assemblin (EC 3.4.21.97) (Protease); Assembly protein (Capsid assembly protein)]
[UL80 APNG] Capsid scaffolding protein (Capsid protein P40) (Protease precursor) (pPR) [Cleaved into: Assemblin (EC 3.4.21.97) (Protease); Assembly protein (Capsid assembly protein)]
[UL80 APNG] Capsid scaffolding protein (Protease precursor) (pPR) [Cleaved into: Assemblin (EC 3.4.21.97) (Protease); Assembly protein (Capsid assembly protein)]
[U53 0R XKRF1] Capsid scaffolding protein (Capsid protein P40) (Protease precursor) (pPR) [Cleaved into: Assemblin (EC 3.4.21.97) (Protease); Assembly protein (Capsid assembly protein)]
[NP] Nucleoprotein (Nucleocapsid protein) (Protein N)
[UL26] Capsid scaffolding protein (Capsid protein P40) (Protease precursor) (pPR) [Cleaved into: Assemblin (EC 3.4.21.97) (Protease); Assembly protein (Capsid assembly protein)]
[35] Capsid scaffolding protein (Capsid protein P40) (Protease precursor) (pPR) (Virion structural gene 35 protein) [Cleaved into: Assemblin (EC 3.4.21.97) (Capsid protein VP24) (Protease); Assembly protein (Capsid assembly protein) (Capsid protein VP22A)]
[VP35 vp35 VP40] Polymerase cofactor VP35

Bibliography :
[28580138] Plasma membrane association facilitates conformational changes in the Marburg virus protein VP40 dimer.