GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Mitogen-activated protein kinase 3 (MAP kinase 3) (MAPK 3) (EC 2.7.11.24) (ERT2) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase isoform p44) (p44-MAPK) (MNK1) (Microtubule-associated protein 2 kinase) (p44-ERK1)

 MK03_RAT                Reviewed;         380 AA.
P21708; Q4PIY8; Q62686; Q9JJ13;
01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
23-JAN-2007, sequence version 5.
17-JUN-2020, entry version 216.
RecName: Full=Mitogen-activated protein kinase 3;
Short=MAP kinase 3;
Short=MAPK 3;
EC=2.7.11.24;
AltName: Full=ERT2;
AltName: Full=Extracellular signal-regulated kinase 1;
Short=ERK-1;
AltName: Full=Insulin-stimulated MAP2 kinase;
AltName: Full=MAP kinase isoform p44;
Short=p44-MAPK;
AltName: Full=MNK1;
AltName: Full=Microtubule-associated protein 2 kinase;
AltName: Full=p44-ERK1;
Name=Mapk3; Synonyms=Erk1, Prkm3;
Rattus norvegicus (Rat).
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
Murinae; Rattus.
NCBI_TaxID=10116;
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
STRAIN=Sprague-Dawley; TISSUE=Brain;
PubMed=1327976; DOI=10.1016/0378-1119(92)90109-3;
Marquardt B., Stabel S.;
"Sequence of a rat cDNA encoding the ERK1-MAP kinase.";
Gene 120:297-299(1992).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
PubMed=10748187; DOI=10.1074/jbc.m910060199;
Yung Y., Yao Z., Hanoch T., Seger R.;
"ERK1b, a 46-kDa ERK isoform that is differentially regulated by MEK.";
J. Biol. Chem. 275:15799-15808(2000).
[3]
PROTEIN SEQUENCE OF 2-65; 96-132; 157-209; 213-221; 280-357 AND 361-380
(ISOFORM 2), CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2,
PHOSPHORYLATION AT THR-203 AND TYR-205, AND IDENTIFICATION BY MASS
SPECTROMETRY.
TISSUE=Pheochromocytoma;
Bienvenut W.V., von Kriegsheim A.F., Kolch W.;
Submitted (AUG-2006) to UniProtKB.
[4]
NUCLEOTIDE SEQUENCE [MRNA] OF 7-380 (ISOFORM 1).
Maisonpierre P.C., le Beau M.M., Espinosa R. III, Ip N.Y., Belluscio L.,
la Monte S.M., Squinto S., Furth M.E., Yancopoulos G.D.;
Submitted (JUL-1991) to the EMBL/GenBank/DDBJ databases.
[5]
PROTEIN SEQUENCE OF 73-84 AND 183-190, AND IDENTIFICATION BY MASS
SPECTROMETRY.
STRAIN=Sprague-Dawley; TISSUE=Brain, and Spinal cord;
Lubec G., Afjehi-Sadat L., Kang S.U.;
Submitted (JUL-2007) to UniProtKB.
[6]
NUCLEOTIDE SEQUENCE [MRNA] OF 14-380 (ISOFORM 1), AND PARTIAL PROTEIN
SEQUENCE.
PubMed=2164259; DOI=10.1126/science.2164259;
Boulton T.G., Yancopoulos G.D., Gregory J.S., Slaughter C., Moomaw C.,
Hsu J., Cobb M.H.;
"An insulin-stimulated protein kinase similar to yeast kinases involved in
cell cycle control.";
Science 249:64-67(1990).
[7]
NUCLEOTIDE SEQUENCE [MRNA] OF 14-380 (ISOFORM 1).
TISSUE=Brain cortex;
PubMed=1716439; DOI=10.1089/dna.1991.10.505;
de Miguel C., Kligman D., Patel J., Detera-Wadleigh S.D.;
"Molecular analysis of microtubule-associated protein-2 kinase cDNA from
mouse and rat brain.";
DNA Cell Biol. 10:505-514(1991).
[8]
PROTEIN SEQUENCE OF 43-64 AND 167-185, AND CHARACTERIZATION.
PubMed=1846291; DOI=10.1021/bi00215a038;
Boulton T.G., Gregory J.S., Cobb M.H.;
"Purification and properties of extracellular signal-regulated kinase 1, an
insulin-stimulated microtubule-associated protein 2 kinase.";
Biochemistry 30:278-286(1991).
[9]
AUTOPHOSPHORYLATION.
PubMed=1712480; DOI=10.1073/pnas.88.14.6142;
Seger R., Ahn N.G., Boulton T.G., Yancopoulos G.D., Panayotatos N.,
Radziejewska E., Ericsson L., Bratlien R.L., Cobb M.H., Krebs E.G.;
"Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo
autophosphorylation on both tyrosine and threonine residues: implications
for their mechanism of activation.";
Proc. Natl. Acad. Sci. U.S.A. 88:6142-6146(1991).
[10]
PHOSPHORYLATION OF EIF4EBP1.
PubMed=7939721; DOI=10.1126/science.7939721;
Lin T.-A., Kong X., Haystead T.A.J., Pause A., Belsham G.J., Sonenberg N.,
Lawrence J.C. Jr.;
"PHAS-I as a link between mitogen-activated protein kinase and translation
initiation.";
Science 266:653-656(1994).
[11]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-203 AND TYR-205, AND
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
PubMed=16641100; DOI=10.1073/pnas.0600895103;
Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.;
"Quantitative phosphoproteomics of vasopressin-sensitive renal cells:
regulation of aquaporin-2 phosphorylation at two sites.";
Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006).
[12]
IDENTIFICATION IN A COMPLEX WITH BRAF; HRAS; MAP2K1 AND RGS14.
PubMed=19319189; DOI=10.1371/journal.pone.0004884;
Willard F.S., Willard M.D., Kimple A.J., Soundararajan M., Oestreich E.A.,
Li X., Sowa N.A., Kimple R.J., Doyle D.A., Der C.J., Zylka M.J.,
Snider W.D., Siderovski D.P.;
"Regulator of G-protein signaling 14 (RGS14) is a selective H-Ras
effector.";
PLoS ONE 4:E4884-E4884(2009).
[13]
REVIEW ON FUNCTION.
PubMed=16393692; DOI=10.1080/02699050500284218;
Yoon S., Seger R.;
"The extracellular signal-regulated kinase: multiple substrates regulate
diverse cellular functions.";
Growth Factors 24:21-44(2006).
[14]
REVIEW ON FUNCTION, AND REVIEW ON SUBCELLULAR LOCATION.
PubMed=19565474; DOI=10.1002/biof.52;
Yao Z., Seger R.;
"The ERK signaling cascade--views from different subcellular
compartments.";
BioFactors 35:407-416(2009).
[15]
REVIEW ON ACTIVITY REGULATION, AND REVIEW ON FUNCTION.
PubMed=21779493; DOI=10.1177/1947601911407328;
Wortzel I., Seger R.;
"The ERK cascade: distinct functions within various subcellular
organelles.";
Genes Cancer 2:195-209(2011).
[16]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-203 AND TYR-205, AND
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
PubMed=22673903; DOI=10.1038/ncomms1871;
Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
Olsen J.V.;
"Quantitative maps of protein phosphorylation sites across 14 different rat
organs and tissues.";
Nat. Commun. 3:876-876(2012).
[17]
SUBCELLULAR LOCATION, AND INTERACTION WITH CAVIN4.
PubMed=24567387; DOI=10.1073/pnas.1315359111;
Ogata T., Naito D., Nakanishi N., Hayashi Y.K., Taniguchi T., Miyagawa K.,
Hamaoka T., Maruyama N., Matoba S., Ikeda K., Yamada H., Oh H., Ueyama T.;
"MURC/Cavin-4 facilitates recruitment of ERK to caveolae and concentric
cardiac hypertrophy induced by alpha1-adrenergic receptors.";
Proc. Natl. Acad. Sci. U.S.A. 111:3811-3816(2014).
-!- FUNCTION: Serine/threonine kinase which acts as an essential component
of the MAP kinase signal transduction pathway. MAPK1/ERK2 and
MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK
cascade. They participate also in a signaling cascade initiated by
activated KIT and KITLG/SCF. Depending on the cellular context, the
MAPK/ERK cascade mediates diverse biological functions such as cell
growth, adhesion, survival and differentiation through the regulation
of transcription, translation, cytoskeletal rearrangements. The
MAPK/ERK cascade plays also a role in initiation and regulation of
meiosis, mitosis, and postmitotic functions in differentiated cells by
phosphorylating a number of transcription factors. About 160 substrates
have already been discovered for ERKs. Many of these substrates are
localized in the nucleus, and seem to participate in the regulation of
transcription upon stimulation. However, other substrates are found in
the cytosol as well as in other cellular organelles, and those are
responsible for processes such as translation, mitosis and apoptosis.
Moreover, the MAPK/ERK cascade is also involved in the regulation of
the endosomal dynamics, including lysosome processing and endosome
cycling through the perinuclear recycling compartment (PNRC); as well
as in the fragmentation of the Golgi apparatus during mitosis. The
substrates include transcription factors (such as ATF2, BCL6, ELK1,
ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN,
GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such
as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of
translation (such as EIF4EBP1) and a variety of other signaling-related
molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1,
RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK,
MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or
MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are
other substrates which enable the propagation the MAPK/ERK signal to
additional cytosolic and nuclear targets, thereby extending the
specificity of the cascade.
-!- CATALYTIC ACTIVITY:
Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.24;
-!- CATALYTIC ACTIVITY:
Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
EC=2.7.11.24;
-!- COFACTOR:
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
-!- ACTIVITY REGULATION: Phosphorylated by MAP2K1/MEK1 and MAP2K2/MEK2 on
Thr-203 and Tyr-205 in response to external stimuli like insulin or
NGF. Both phosphorylations are required for activity. This
phosphorylation causes dramatic conformational changes, which enable
full activation and interaction of MAPK1/ERK2 with its substrates.
Dephosphorylated and inactivated by DUSP3, DUSP6 and DUSP9.
-!- SUBUNIT: Binds both upstream activators and downstream substrates in
multimolecular complexes. Found in a complex with at least BRAF, HRAS,
MAP2K1/MEK1, MAPK3 and RGS14. Interacts with ADAM15, ARRB2, CANX, DAPK1
(via death domain), HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, PEA15, SGK1
and MKNK2. MKNK2 isoform 1 binding prevents from dephosphorylation and
inactivation. Interacts with TPR. Interacts with HSF1 (via D domain and
preferentially with hyperphosphorylated form); this interaction occurs
upon heat shock. Interacts with CDKN2AIP (By similarity). Interacts
with CAVIN4 (PubMed:24567387). {ECO:0000250|UniProtKB:P27361,
ECO:0000250|UniProtKB:Q63844, ECO:0000269|PubMed:24567387}.
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24567387}. Nucleus.
Membrane, caveola {ECO:0000269|PubMed:24567387}.
Note=Autophosphorylation at Thr-207 promotes nuclear localization.
PEA15-binding redirects the biological outcome of MAPK3 kinase-
signaling by sequestering MAPK3 into the cytoplasm. {ECO:0000250}.
-!- SUBCELLULAR LOCATION: [Isoform 2]: Nucleus.
-!- ALTERNATIVE PRODUCTS:
Event=Alternative splicing; Named isoforms=2;
Name=1; Synonyms=A;
IsoId=P21708-1; Sequence=Displayed;
Name=2; Synonyms=B;
IsoId=P21708-2; Sequence=VSP_004830;
-!- TISSUE SPECIFICITY: Highest levels within the nervous system, expressed
in different tissues, mostly in intestine, placenta and lung.
-!- DEVELOPMENTAL STAGE: Increased expression during development.
-!- DOMAIN: The TXY motif contains the threonine and tyrosine residues
whose phosphorylation activates the MAP kinases.
-!- PTM: Phosphorylated upon FLT3 and KIT signaling. Ligand-activated ALK
induces tyrosine phosphorylation (By similarity). Dephosphorylated by
PTPRJ at Tyr-205 (By similarity). Dually phosphorylated on Thr-203 and
Tyr-205, which activates the enzyme. {ECO:0000250,
ECO:0000269|PubMed:7939721, ECO:0000269|Ref.3}.
-!- SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr
protein kinase family. MAP kinase subfamily. {ECO:0000305}.
---------------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
---------------------------------------------------------------------------
EMBL; S46779; AAA11604.1; -; mRNA.
EMBL; X65198; CAA46318.1; -; mRNA.
EMBL; AF155236; AAF71666.1; -; mRNA.
EMBL; M61177; AAA63486.1; -; mRNA.
EMBL; M38194; AAA41123.1; -; mRNA.
EMBL; U12008; AAA20009.1; -; mRNA.
PIR; JC1451; JC1451.
RefSeq; NP_059043.1; NM_017347.2.
SMR; P21708; -.
BioGRID; 248427; 5.
CORUM; P21708; -.
DIP; DIP-487N; -.
IntAct; P21708; 271.
MINT; P21708; -.
STRING; 10116.ENSRNOP00000026627; -.
ChEMBL; CHEMBL5809; -.
iPTMnet; P21708; -.
PhosphoSitePlus; P21708; -.
SwissPalm; P21708; -.
jPOST; P21708; -.
PaxDb; P21708; -.
PRIDE; P21708; -.
Ensembl; ENSRNOT00000087625; ENSRNOP00000070784; ENSRNOG00000053583. [P21708-1]
GeneID; 50689; -.
KEGG; rno:50689; -.
UCSC; RGD:3046; rat. [P21708-1]
CTD; 5595; -.
RGD; 3046; Mapk3.
eggNOG; KOG0660; Eukaryota.
eggNOG; ENOG410XNY0; LUCA.
GeneTree; ENSGT00940000160691; -.
HOGENOM; CLU_000288_181_1_1; -.
InParanoid; P21708; -.
KO; K04371; -.
OMA; RKRKPFA; -.
OrthoDB; 741207at2759; -.
PhylomeDB; P21708; -.
TreeFam; TF105097; -.
BRENDA; 2.7.11.24; 5301.
Reactome; R-RNO-110056; MAPK3 (ERK1) activation.
Reactome; R-RNO-112409; RAF-independent MAPK1/3 activation.
Reactome; R-RNO-1169408; ISG15 antiviral mechanism.
Reactome; R-RNO-1295596; Spry regulation of FGF signaling.
Reactome; R-RNO-162658; Golgi Cisternae Pericentriolar Stack Reorganization.
Reactome; R-RNO-170968; Frs2-mediated activation.
Reactome; R-RNO-198753; ERK/MAPK targets.
Reactome; R-RNO-202670; ERKs are inactivated.
Reactome; R-RNO-2029482; Regulation of actin dynamics for phagocytic cup formation.
Reactome; R-RNO-2559580; Oxidative Stress Induced Senescence.
Reactome; R-RNO-2559582; Senescence-Associated Secretory Phenotype (SASP).
Reactome; R-RNO-2559585; Oncogene Induced Senescence.
Reactome; R-RNO-2871796; FCERI mediated MAPK activation.
Reactome; R-RNO-3371453; Regulation of HSF1-mediated heat shock response.
Reactome; R-RNO-375165; NCAM signaling for neurite out-growth.
Reactome; R-RNO-445144; Signal transduction by L1.
Reactome; R-RNO-450341; Activation of the AP-1 family of transcription factors.
Reactome; R-RNO-456926; Thrombin signalling through proteinase activated receptors (PARs).
Reactome; R-RNO-5654726; Negative regulation of FGFR1 signaling.
Reactome; R-RNO-5654727; Negative regulation of FGFR2 signaling.
Reactome; R-RNO-5654732; Negative regulation of FGFR3 signaling.
Reactome; R-RNO-5654733; Negative regulation of FGFR4 signaling.
Reactome; R-RNO-5663213; RHO GTPases Activate WASPs and WAVEs.
Reactome; R-RNO-5668599; RHO GTPases Activate NADPH Oxidases.
Reactome; R-RNO-5673001; RAF/MAP kinase cascade.
Reactome; R-RNO-5674135; MAP2K and MAPK activation.
Reactome; R-RNO-5674499; Negative feedback regulation of MAPK pathway.
Reactome; R-RNO-5675221; Negative regulation of MAPK pathway.
Reactome; R-RNO-6811558; PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
Reactome; R-RNO-73728; RNA Polymerase I Promoter Opening.
Reactome; R-RNO-74749; Signal attenuation.
Reactome; R-RNO-881907; Gastrin-CREB signalling pathway via PKC and MAPK.
Reactome; R-RNO-8939211; ESR-mediated signaling.
Reactome; R-RNO-982772; Growth hormone receptor signaling.
PRO; PR:P21708; -.
Proteomes; UP000002494; Chromosome 1.
Bgee; ENSRNOG00000053583; Expressed in colon and 9 other tissues.
Genevisible; P21708; RN.
GO; GO:0005901; C:caveola; IDA:UniProtKB.
GO; GO:0005623; C:cell; IEA:GOC.
GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
GO; GO:0005856; C:cytoskeleton; TAS:UniProtKB.
GO; GO:0005829; C:cytosol; IDA:RGD.
GO; GO:0005769; C:early endosome; TAS:UniProtKB.
GO; GO:0005925; C:focal adhesion; TAS:UniProtKB.
GO; GO:0005794; C:Golgi apparatus; TAS:UniProtKB.
GO; GO:0005770; C:late endosome; TAS:UniProtKB.
GO; GO:0005739; C:mitochondrion; TAS:UniProtKB.
GO; GO:0005635; C:nuclear envelope; ISO:RGD.
GO; GO:0005654; C:nucleoplasm; IDA:UniProtKB.
GO; GO:0005634; C:nucleus; IDA:RGD.
GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
GO; GO:0032991; C:protein-containing complex; IDA:RGD.
GO; GO:0031143; C:pseudopodium; ISO:RGD.
GO; GO:0005524; F:ATP binding; IDA:RGD.
GO; GO:0042802; F:identical protein binding; ISO:RGD.
GO; GO:0004707; F:MAP kinase activity; IDA:UniProtKB.
GO; GO:0004708; F:MAP kinase kinase activity; ISO:RGD.
GO; GO:0019902; F:phosphatase binding; ISO:RGD.
GO; GO:0001784; F:phosphotyrosine residue binding; ISO:RGD.
GO; GO:0004672; F:protein kinase activity; ISO:RGD.
GO; GO:0004674; F:protein serine/threonine kinase activity; IC:RGD.
GO; GO:0097110; F:scaffold protein binding; IPI:BHF-UCL.
GO; GO:0007568; P:aging; IDA:RGD.
GO; GO:0009887; P:animal organ morphogenesis; ISO:RGD.
GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
GO; GO:0019369; P:arachidonic acid metabolic process; IEP:RGD.
GO; GO:0060020; P:Bergmann glial cell differentiation; ISO:RGD.
GO; GO:0030509; P:BMP signaling pathway; ISO:RGD.
GO; GO:0061308; P:cardiac neural crest cell development involved in heart development; ISO:RGD.
GO; GO:0051216; P:cartilage development; ISO:RGD.
GO; GO:0072584; P:caveolin-mediated endocytosis; TAS:UniProtKB.
GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
GO; GO:0007166; P:cell surface receptor signaling pathway; IBA:GO_Central.
GO; GO:0034198; P:cellular response to amino acid starvation; ISO:RGD.
GO; GO:0071276; P:cellular response to cadmium ion; ISO:RGD.
GO; GO:0006974; P:cellular response to DNA damage stimulus; ISO:RGD.
GO; GO:1903351; P:cellular response to dopamine; ISO:RGD.
GO; GO:0071260; P:cellular response to mechanical stimulus; ISO:RGD.
GO; GO:0071310; P:cellular response to organic substance; IBA:GO_Central.
GO; GO:0034614; P:cellular response to reactive oxygen species; ISO:RGD.
GO; GO:0071356; P:cellular response to tumor necrosis factor; ISO:RGD.
GO; GO:0046697; P:decidualization; IDA:RGD.
GO; GO:0006975; P:DNA damage induced protein phosphorylation; ISO:RGD.
GO; GO:0070371; P:ERK1 and ERK2 cascade; ISO:RGD.
GO; GO:0060324; P:face development; ISO:RGD.
GO; GO:0070498; P:interleukin-1-mediated signaling pathway; ISO:RGD.
GO; GO:0035556; P:intracellular signal transduction; IDA:RGD.
GO; GO:0031663; P:lipopolysaccharide-mediated signaling pathway; ISO:RGD.
GO; GO:0060425; P:lung morphogenesis; ISO:RGD.
GO; GO:0000165; P:MAPK cascade; IMP:RGD.
GO; GO:2000657; P:negative regulation of apolipoprotein binding; ISO:RGD.
GO; GO:0014032; P:neural crest cell development; ISO:RGD.
GO; GO:0042473; P:outer ear morphogenesis; ISO:RGD.
GO; GO:0018105; P:peptidyl-serine phosphorylation; IMP:RGD.
GO; GO:0038083; P:peptidyl-tyrosine autophosphorylation; ISO:RGD.
GO; GO:0016310; P:phosphorylation; ISO:RGD.
GO; GO:0031281; P:positive regulation of cyclase activity; ISO:RGD.
GO; GO:0002741; P:positive regulation of cytokine secretion involved in immune response; ISO:RGD.
GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISO:RGD.
GO; GO:0010628; P:positive regulation of gene expression; ISO:RGD.
GO; GO:0035066; P:positive regulation of histone acetylation; ISO:RGD.
GO; GO:0033129; P:positive regulation of histone phosphorylation; ISO:RGD.
GO; GO:0010759; P:positive regulation of macrophage chemotaxis; ISO:RGD.
GO; GO:1905050; P:positive regulation of metallopeptidase activity; ISO:RGD.
GO; GO:0001934; P:positive regulation of protein phosphorylation; ISO:RGD.
GO; GO:0051973; P:positive regulation of telomerase activity; ISO:RGD.
GO; GO:1904355; P:positive regulation of telomere capping; ISO:RGD.
GO; GO:0032212; P:positive regulation of telomere maintenance via telomerase; ISO:RGD.
GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
GO; GO:0045727; P:positive regulation of translation; IMP:RGD.
GO; GO:1904417; P:positive regulation of xenophagy; ISO:RGD.
GO; GO:0006468; P:protein phosphorylation; IDA:RGD.
GO; GO:0065003; P:protein-containing complex assembly; IMP:UniProtKB.
GO; GO:0030641; P:regulation of cellular pH; ISO:RGD.
GO; GO:0051493; P:regulation of cytoskeleton organization; TAS:UniProtKB.
GO; GO:0051090; P:regulation of DNA-binding transcription factor activity; ISO:RGD.
GO; GO:2000641; P:regulation of early endosome to late endosome transport; TAS:UniProtKB.
GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
GO; GO:0090170; P:regulation of Golgi inheritance; TAS:UniProtKB.
GO; GO:0030278; P:regulation of ossification; ISO:RGD.
GO; GO:0032872; P:regulation of stress-activated MAPK cascade; TAS:UniProtKB.
GO; GO:0070849; P:response to epidermal growth factor; ISS:UniProtKB.
GO; GO:0043330; P:response to exogenous dsRNA; ISO:RGD.
GO; GO:0032496; P:response to lipopolysaccharide; ISO:RGD.
GO; GO:0009636; P:response to toxic substance; IDA:RGD.
GO; GO:0019233; P:sensory perception of pain; ISO:RGD.
GO; GO:0051403; P:stress-activated MAPK cascade; ISO:RGD.
GO; GO:0048538; P:thymus development; ISO:RGD.
GO; GO:0030878; P:thyroid gland development; ISO:RGD.
GO; GO:0060440; P:trachea formation; ISO:RGD.
GO; GO:0006351; P:transcription, DNA-templated; ISO:RGD.
InterPro; IPR011009; Kinase-like_dom_sf.
InterPro; IPR003527; MAP_kinase_CS.
InterPro; IPR008349; MAPK_ERK1/2.
InterPro; IPR000719; Prot_kinase_dom.
InterPro; IPR017441; Protein_kinase_ATP_BS.
InterPro; IPR008271; Ser/Thr_kinase_AS.
Pfam; PF00069; Pkinase; 1.
PRINTS; PR01770; ERK1ERK2MAPK.
SMART; SM00220; S_TKc; 1.
SUPFAM; SSF56112; SSF56112; 1.
PROSITE; PS01351; MAPK; 1.
PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
1: Evidence at protein level;
Acetylation; Alternative splicing; Apoptosis; ATP-binding; Cell cycle;
Cytoplasm; Direct protein sequencing; Kinase; Membrane; Nucleotide-binding;
Nucleus; Phosphoprotein; Reference proteome;
Serine/threonine-protein kinase; Transferase.
INIT_MET 1
/note="Removed"
/evidence="ECO:0000269|Ref.3"
CHAIN 2..380
/note="Mitogen-activated protein kinase 3"
/id="PRO_0000186253"
DOMAIN 43..331
/note="Protein kinase"
/evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
NP_BIND 49..57
/note="ATP"
/evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
MOTIF 203..205
/note="TXY"
ACT_SITE 167
/note="Proton acceptor"
/evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
ECO:0000255|PROSITE-ProRule:PRU10027"
BINDING 72
/note="ATP"
/evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
MOD_RES 2
/note="N-acetylalanine"
/evidence="ECO:0000269|Ref.3"
MOD_RES 199
/note="Phosphothreonine"
/evidence="ECO:0000250|UniProtKB:P27361"
MOD_RES 203
/note="Phosphothreonine; by MAP2K1 and MAP2K2"
/evidence="ECO:0000244|PubMed:16641100,
ECO:0000244|PubMed:22673903, ECO:0000269|Ref.3"
MOD_RES 205
/note="Phosphotyrosine; by MAP2K1 and MAP2K2"
/evidence="ECO:0000244|PubMed:16641100,
ECO:0000244|PubMed:22673903, ECO:0000269|Ref.3"
MOD_RES 208
/note="Phosphothreonine; by autocatalysis"
/evidence="ECO:0000250|UniProtKB:P27361"
VAR_SEQ 340
/note="E -> EVSRPPAAGRGISVPSVRPVPYCLCPQ (in isoform 2)"
/evidence="ECO:0000303|PubMed:10748187"
/id="VSP_004830"
CONFLICT 95
/note="R -> G (in Ref. 1; AAA11604/CAA46318, 4; AAA63486
and 7; AAA20009)"
/evidence="ECO:0000305"
SEQUENCE 380 AA; 43081 MW; 49C4EA95B627237F CRC64;
MAAAAAAPGG GGGEPRGTAG VVPVVPGEVE VVKGQPFDVG PRYTQLQYIG EGAYGMVSSA
YDHVRKTRVA IKKISPFEHQ TYCQRTLREI QILLRFRHEN VIGIRDILRA PTLEAMRDVY
IVQDLMETDL YKLLKSQQLS NDHICYFLYQ ILRGLKYIHS ANVLHRDLKP SNLLINTTCD
LKICDFGLAR IADPEHDHTG FLTEYVATRW YRAPEIMLNS KGYTKSIDIW SVGCILAEML
SNRPIFPGKH YLDQLNHILG ILGSPSQEDL NCIINMKARN YLQSLPSKTK VAWAKLFPKS
DSKALDLLDR MLTFNPNKRI TVEEALAHPY LEQYYDPTDE PVAEEPFTFD MELDDLPKER
LKELIFQETA RFQPGAPEAP


Related products :

Catalog number Product name Quantity
15-288-22781 Mitogen-activated protein kinase 3 - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associate 0.1 mg
10-782-55053 Mitogen-activated protein kinase 3 - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associate 0.05 mg
10-782-55052 Mitogen-activated protein kinase 3 - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associate 0.005 mg
10-782-55053 Mitogen-activated protein kinase 3 - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associate 0.02 mg
10-782-55052 Mitogen-activated protein kinase 3 - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associate 0.02 mg
10-782-55052 Mitogen-activated protein kinase 3 - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associate 0.01 mg
15-288-22781 Mitogen-activated protein kinase 3 - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associate 0.05 mg
10-782-55052 Mitogen-activated protein kinase 3 - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associate 0.001 mg
U1357r CLIA Erk1,ERK-1,ERT2,Extracellular signal-regulated kinase 1,Insulin-stimulated MAP2 kinase,MAP kinase 1,MAP kinase 3,MAP kinase isoform p44,MAPK 1,MAPK 3,Mapk3,Microtubule-associated protein 2 kinase 96T
U1357m CLIA Erk1,ERK-1,ERT2,Extracellular signal-regulated kinase 1,Insulin-stimulated MAP2 kinase,MAP kinase 1,MAP kinase 3,MAP kinase isoform p44,MAPK 1,MAPK 3,Mapk3,Microtubule-associated protein 2 kinase 96T
E1357r ELISA kit Erk1,ERK-1,ERT2,Extracellular signal-regulated kinase 1,Insulin-stimulated MAP2 kinase,MAP kinase 1,MAP kinase 3,MAP kinase isoform p44,MAPK 1,MAPK 3,Mapk3,Microtubule-associated protein 2 96T
E1357m ELISA kit Erk1,ERK-1,ERT2,Extracellular signal-regulated kinase 1,Insulin-stimulated MAP2 kinase,MAP kinase 1,MAP kinase 3,MAP kinase isoform p44,MAPK 1,MAPK 3,Mapk3,Microtubule-associated protein 2 96T
E1357r ELISA Erk1,ERK-1,ERT2,Extracellular signal-regulated kinase 1,Insulin-stimulated MAP2 kinase,MAP kinase 1,MAP kinase 3,MAP kinase isoform p44,MAPK 1,MAPK 3,Mapk3,Microtubule-associated protein 2 kinas 96T
E1357m ELISA Erk1,ERK-1,ERT2,Extracellular signal-regulated kinase 1,Insulin-stimulated MAP2 kinase,MAP kinase 1,MAP kinase 3,MAP kinase isoform p44,MAPK 1,MAPK 3,Mapk3,Microtubule-associated protein 2 kinas 96T
E1357h ELISA kit ERK1,ERK-1,ERT2,Extracellular signal-regulated kinase 1,Homo sapiens,Human,Insulin-stimulated MAP2 kinase,MAP kinase 1,MAP kinase 3,MAP kinase isoform p44,MAPK 1,MAPK 3,MAPK3,Microtubule-as 96T
U1357h CLIA ERK1,ERK-1,ERT2,Extracellular signal-regulated kinase 1,Homo sapiens,Human,Insulin-stimulated MAP2 kinase,MAP kinase 1,MAP kinase 3,MAP kinase isoform p44,MAPK 1,MAPK 3,MAPK3,Microtubule-associat 96T
E1357h ELISA ERK1,ERK-1,ERT2,Extracellular signal-regulated kinase 1,Homo sapiens,Human,Insulin-stimulated MAP2 kinase,MAP kinase 1,MAP kinase 3,MAP kinase isoform p44,MAPK 1,MAPK 3,MAPK3,Microtubule-associa 96T
18-785-210145 p44_42 MAP Kinase (Ab-204) - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associated protei 0.1 mg
18-785-210145 p44_42 MAP Kinase (Ab-204) - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associated protei 0.05 mg
18-785-210144 p44_42 MAP Kinase (Ab-202) - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associated protei 0.1 mg
18-785-210144 p44_42 MAP Kinase (Ab-202) - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associated protei 0.05 mg
18-785-210143 p44_42 MAP Kinase (Phospho-Tyr204) - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associate 0.05 mg
18-785-210142 p44_42 MAP Kinase (Phospho-Thr202) - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associate 0.05 mg
18-785-210142 p44_42 MAP Kinase (Phospho-Thr202) - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associate 0.1 mg
18-785-210143 p44_42 MAP Kinase (Phospho-Tyr204) - EC 2.7.11.24; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK 1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associate 0.1 mg