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NADH-ubiquinone oxidoreductase chain 2 (EC 1.6.5.3) (Fragment)

 K7WVB6_9TELE            Unreviewed;       348 AA.
K7WVB6;
06-FEB-2013, integrated into UniProtKB/TrEMBL.
06-FEB-2013, sequence version 1.
13-FEB-2019, entry version 28.
RecName: Full=NADH-ubiquinone oxidoreductase chain 2 {ECO:0000256|RuleBase:RU003403, ECO:0000256|SAAS:SAAS00387861};
EC=7.1.1.2 {ECO:0000256|RuleBase:RU003403, ECO:0000256|SAAS:SAAS01109717};
Flags: Fragment;
Name=nd2 {ECO:0000313|EMBL:AFX67532.1};
Chilatherina sentaniensis (Sentani rainbowfish).
Mitochondrion {ECO:0000313|EMBL:AFX67532.1}.
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
Ovalentaria; Atherinomorphae; Atheriniformes; Melanotaeniidae;
Chilatherina.
NCBI_TaxID=1250779 {ECO:0000313|EMBL:AFX67532.1};
[1] {ECO:0000313|EMBL:AFX67532.1}
NUCLEOTIDE SEQUENCE.
STRAIN=Csent.real.exJG.1 {ECO:0000313|EMBL:AFX67532.1};
PubMed=23313459; DOI=10.1016/j.ympev.2012.12.019;
Unmack P.J., Allen G.R., Johnson J.B.;
"Phylogeny and biogeography of rainbowfishes (Melanotaeniidae) from
Australia and New Guinea.";
Mol. Phylogenet. Evol. 67:15-27(2013).
-!- FUNCTION: Core subunit of the mitochondrial membrane respiratory
chain NADH dehydrogenase (Complex I) that is believed to belong to
the minimal assembly required for catalysis. Complex I functions
in the transfer of electrons from NADH to the respiratory chain.
The immediate electron acceptor for the enzyme is believed to be
ubiquinone. {ECO:0000256|RuleBase:RU003403,
ECO:0000256|SAAS:SAAS00093760}.
-!- CATALYTIC ACTIVITY:
Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4
H(+)(out) + NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565,
Rhea:RHEA-COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389,
ChEBI:CHEBI:17976, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
EC=7.1.1.2; Evidence={ECO:0000256|RuleBase:RU003403,
ECO:0000256|SAAS:SAAS01125045};
-!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
{ECO:0000256|RuleBase:RU003403, ECO:0000256|SAAS:SAAS00093751};
Multi-pass membrane protein {ECO:0000256|RuleBase:RU003403,
ECO:0000256|SAAS:SAAS00093751}.
-!- SIMILARITY: Belongs to the complex I subunit 2 family.
{ECO:0000256|RuleBase:RU003403, ECO:0000256|SAAS:SAAS00573211}.
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EMBL; KC133720; AFX67532.1; -; Genomic_DNA.
GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro.
InterPro; IPR010933; NADH_DH_su2_C.
InterPro; IPR003917; NADH_UbQ_OxRdtase_chain2.
InterPro; IPR001750; ND/Mrp_mem.
Pfam; PF06444; NADH_dehy_S2_C; 1.
Pfam; PF00361; Proton_antipo_M; 1.
PRINTS; PR01436; NADHDHGNASE2.
3: Inferred from homology;
Electron transport {ECO:0000256|RuleBase:RU003403,
ECO:0000256|SAAS:SAAS00093685};
Membrane {ECO:0000256|RuleBase:RU003403,
ECO:0000256|SAAS:SAAS00464436, ECO:0000256|SAAS:SAAS00464584};
Mitochondrion {ECO:0000256|RuleBase:RU003403,
ECO:0000256|SAAS:SAAS00464436};
Mitochondrion inner membrane {ECO:0000256|RuleBase:RU003403,
ECO:0000256|SAAS:SAAS00464436};
NAD {ECO:0000256|RuleBase:RU003403, ECO:0000256|SAAS:SAAS00093707};
Respiratory chain {ECO:0000256|RuleBase:RU003403,
ECO:0000256|SAAS:SAAS00093685};
Translocase {ECO:0000256|RuleBase:RU003403,
ECO:0000256|SAAS:SAAS01109684};
Transmembrane {ECO:0000256|RuleBase:RU003403,
ECO:0000256|SAAS:SAAS00464584};
Transmembrane helix {ECO:0000256|RuleBase:RU003403,
ECO:0000256|SAAS:SAAS00464584};
Transport {ECO:0000256|RuleBase:RU003403,
ECO:0000256|SAAS:SAAS00093685};
Ubiquinone {ECO:0000256|RuleBase:RU003403,
ECO:0000256|SAAS:SAAS00093675}.
TRANSMEM 60 80 Helical. {ECO:0000256|RuleBase:RU003403}.
TRANSMEM 92 115 Helical. {ECO:0000256|RuleBase:RU003403}.
TRANSMEM 151 169 Helical. {ECO:0000256|RuleBase:RU003403}.
TRANSMEM 190 216 Helical. {ECO:0000256|RuleBase:RU003403}.
TRANSMEM 236 253 Helical. {ECO:0000256|RuleBase:RU003403}.
TRANSMEM 274 293 Helical. {ECO:0000256|RuleBase:RU003403}.
TRANSMEM 323 346 Helical. {ECO:0000256|RuleBase:RU003403}.
DOMAIN 23 286 Proton_antipo_M.
{ECO:0000259|Pfam:PF00361}.
DOMAIN 289 342 NADH_dehy_S2_C.
{ECO:0000259|Pfam:PF06444}.
NON_TER 348 348 {ECO:0000313|EMBL:AFX67532.1}.
SEQUENCE 348 AA; 37843 MW; 4DEB36A92672397F CRC64;
MNPYILMTLA FGLGLGTLIT LTSSHWLLAW MGLEMNTLAI IPLLAQHHHP RATEATTKYF
LTQATAAAML LFATTTNAWL SGQWEIQQMT HPLPTTIITL ALALKVGLAP LHAWLPEVLQ
GLDLTAGLIL STWQKIAPFA LLLQINAPTP LMLISLSLLS ILVGGWGGLN QNQLRKILAY
SSIAHLGWMV LVLQFSPSLT LLTLSLYIIM TSSLFLSFKT NKTTTVNSLA TSWAKTPTLT
LLIPPILLSL GGLPPLTGFM PKWLILQELT KQDLALAATM AALSALLSLY FYLRLCYAMT
LTVAPNTITN ITPWRLPSTQ LSLPLSVTAC LTICLLPLTP SILTIATL


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Kits Elisa; taq POLYMERASE

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