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Negative elongation factor D

 NELFD_DROME             Reviewed;         578 AA.
Q24134; Q24553; Q9V418;
14-NOV-2003, integrated into UniProtKB/Swiss-Prot.
14-NOV-2003, sequence version 2.
02-JUN-2021, entry version 135.
RecName: Full=Negative elongation factor D;
Name=TH1; Synonyms=NELF-D; ORFNames=CG9984;
Drosophila melanogaster (Fruit fly).
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
Drosophilidae; Drosophila; Sophophora.
NCBI_TaxID=7227;
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=Berkeley; TISSUE=Embryo;
PubMed=11030415; DOI=10.1007/s004390000344;
Bonthron D.T., Hayward B.E., Moran V., Strain L.;
"Characterization of TH1 and CTSZ, two non-imprinted genes downstream of
GNAS1 in chromosome 20q13.";
Hum. Genet. 107:165-175(2000).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Embryo;
Oliveri D.R., Banga S.S., Boyd J.B.;
"Characterization of a prominent transcript in Drosophila embryos.";
Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Berkeley;
PubMed=10731132; DOI=10.1126/science.287.5461.2185;
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
Venter J.C.;
"The genome sequence of Drosophila melanogaster.";
Science 287:2185-2195(2000).
[4]
GENOME REANNOTATION.
STRAIN=Berkeley;
PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
"Annotation of the Drosophila melanogaster euchromatic genome: a systematic
review.";
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Berkeley; TISSUE=Embryo;
PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
Celniker S.E.;
"A Drosophila full-length cDNA resource.";
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
[6]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-393.
PubMed=7671309; DOI=10.1016/0092-8674(95)90478-6;
Hari K.L., Santerre A., Sekelsky J.J., McKim K.S., Boyd J.B., Hawley R.S.;
"The mei-41 gene of D. melanogaster is a structural and functional homolog
of the human ataxia telangiectasia gene.";
Cell 82:815-821(1995).
[7]
FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH NELF-E.
PubMed=12782658; DOI=10.1101/gad.1091403;
Wu C.-H., Yamaguchi Y., Benjamin L.R., Horvat-Gordon M., Washinsky J.,
Enerly E., Larsson J., Lambertsson A., Handa H., Gilmour D.;
"NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in
Drosophila.";
Genes Dev. 17:1402-1414(2003).
[8]
FUNCTION, AND SUBUNIT.
PubMed=15741180; DOI=10.1093/nar/gki274;
Wu C.-H., Lee C., Fan R., Smith M.J., Yamaguchi Y., Handa H., Gilmour D.S.;
"Molecular characterization of Drosophila NELF.";
Nucleic Acids Res. 33:1269-1279(2005).
-!- FUNCTION: Essential component of the NELF complex, a complex that
negatively regulates the elongation of transcription by RNA polymerase
II by RNA polymerase II. The NELF complex, which acts via an
association with the DSIF complex, causes transcriptional pausing.
{ECO:0000269|PubMed:12782658, ECO:0000269|PubMed:15741180}.
-!- SUBUNIT: Component of the NELF complex, which is at least composed of
TH1/NELF-D and NELF-E. {ECO:0000269|PubMed:15741180}.
-!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12782658}. Chromosome
{ECO:0000269|PubMed:12782658}. Note=Associates with polytene
chromosomes. Associates with the hsp70 promoter when it is inactive,
but not when it is activated. The NELF complex possibly dissociates
from chromatin following phosphorylation of RNA polymerase II.
-!- SIMILARITY: Belongs to the NELF-D family. {ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=AAA28932.1; Type=Frameshift; Evidence={ECO:0000305};
Sequence=AAC46880.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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EMBL; AJ238377; CAB64258.1; -; mRNA.
EMBL; AJ238378; CAB64259.1; -; mRNA.
EMBL; L01790; AAA28932.1; ALT_FRAME; mRNA.
EMBL; AE014298; AAF48603.1; -; Genomic_DNA.
EMBL; AY051957; AAK93381.1; -; mRNA.
EMBL; U34925; AAC46880.1; ALT_SEQ; Genomic_DNA.
PIR; T13287; T13287.
RefSeq; NP_001285326.1; NM_001298397.1.
RefSeq; NP_573123.1; NM_132895.3.
BioGRID; 58947; 7.
DIP; DIP-23874N; -.
IntAct; Q24134; 7.
STRING; 7227.FBpp0074080; -.
PaxDb; Q24134; -.
PRIDE; Q24134; -.
DNASU; 32607; -.
EnsemblMetazoa; FBtr0074305; FBpp0074080; FBgn0010416.
EnsemblMetazoa; FBtr0340315; FBpp0309276; FBgn0010416.
GeneID; 32607; -.
KEGG; dme:Dmel_CG9984; -.
UCSC; CG9984-RA; d. melanogaster.
CTD; 32607; -.
FlyBase; FBgn0010416; TH1.
eggNOG; ENOG502QPUE; Eukaryota.
HOGENOM; CLU_028060_0_0_1; -.
InParanoid; Q24134; -.
OMA; VMEPSYF; -.
OrthoDB; 1288184at2759; -.
PhylomeDB; Q24134; -.
Reactome; R-DME-112382; Formation of RNA Pol II elongation complex.
Reactome; R-DME-113418; Formation of the Early Elongation Complex.
Reactome; R-DME-674695; RNA Polymerase II Pre-transcription Events.
Reactome; R-DME-6796648; TP53 Regulates Transcription of DNA Repair Genes.
Reactome; R-DME-75955; RNA Polymerase II Transcription Elongation.
BioGRID-ORCS; 32607; 0 hits in 1 CRISPR screen.
GenomeRNAi; 32607; -.
PRO; PR:Q24134; -.
Proteomes; UP000000803; Chromosome X.
Bgee; FBgn0010416; Expressed in embryo and 52 other tissues.
ExpressionAtlas; Q24134; baseline and differential.
Genevisible; Q24134; DM.
GO; GO:0032021; C:NELF complex; IPI:FlyBase.
GO; GO:0005634; C:nucleus; IDA:FlyBase.
GO; GO:0005705; C:polytene chromosome interband; IDA:UniProtKB.
GO; GO:0005703; C:polytene chromosome puff; IDA:UniProtKB.
GO; GO:0008023; C:transcription elongation factor complex; IPI:UniProtKB.
GO; GO:0017053; C:transcription repressor complex; IDA:UniProtKB.
GO; GO:0003723; F:RNA binding; IDA:UniProtKB.
GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB.
GO; GO:0034244; P:negative regulation of transcription elongation from RNA polymerase II promoter; IDA:FlyBase.
InterPro; IPR006942; TH1.
PANTHER; PTHR12144; PTHR12144; 1.
Pfam; PF04858; TH1; 1.
1: Evidence at protein level;
Chromosome; Nucleus; Reference proteome; Repressor; Transcription;
Transcription regulation.
CHAIN 1..578
/note="Negative elongation factor D"
/id="PRO_0000219133"
REGION 1..28
/note="Disordered"
/evidence="ECO:0000256|SAM:MobiDB-lite"
COMPBIAS 12..26
/note="Polar residues"
/evidence="ECO:0000256|SAM:MobiDB-lite"
SEQUENCE 578 AA; 65781 MW; 2949EE378AEC19BE CRC64;
MEVEYDDSGW QGRAKGQTNP EETLEDNPQK TIQECLEKFL TPDYIMEPGI FTQLKRYFQS
GGSPEEVISM LSENYKAVAQ MANLLAEWLI LAGVKVTEVQ AMVENHLKEM ILKSFDPKKA
DTIFTEEGET PDWLTEMIDH YTWRSLIYRL AEEYPDCLML NFTIKLISDA GFQSEITSIS
TAAQQIEVFS RVLKTSIVKF LNNPDDVHGA IQECARMVCH GQHTYVYSQV LIQVLSQEQK
GGFNMKRLSQ EIIKYALQNN QNVTPITMAL NGSAVYPQAC QALTSMLTRN TLNPADITVL
FRNYSGSDPP PIDLIRNPQF LELLVDALFR SGVKINPEHK PKYMFLLAYA SAVIDQPAKK
RPMTERMLNK EELKSTIQAI EKAHTICNVD QGSTELIAEL QTLYNCIKYP VVGVGVIRWI
ENVVMEPSYF KLSTDSCPTH LAVLDEVAAV HPTLQQQILF LLIRLFESKQ DELEILVQLE
MKKMILDRMV NLLTRGCVVP VLRYIKQCCA IEDTDISLIR YFVTEVLETI THPYSPEFVQ
LFLPMVENEE ITGTMRGEGD NDPVSEFIVH CKAHYTTV


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Related Genes :
[NELFCD NELFD TH1 TH1L HSPC130] Negative elongation factor C/D (NELF-C/D) (TH1-like protein)
[Nelfe D17h6s45 Rd Rdbp] Negative elongation factor E (NELF-E) (RNA-binding protein RD)
[TH1 NELF-D CG9984] Negative elongation factor D
[NELFE RD RDBP] Negative elongation factor E (NELF-E) (RNA-binding protein RD)
[NELFB COBRA1 KIAA1182] Negative elongation factor B (NELF-B) (Cofactor of BRCA1)
[NELFA WHSC2 P/OKcl.15] Negative elongation factor A (NELF-A) (Wolf-Hirschhorn syndrome candidate 2 protein)
[Nelfb Cobra1 MNCb-5210] Negative elongation factor B (NELF-B) (Cofactor of BRCA1)
[Nelf-E anon-66Da CG5994] Negative elongation factor E
[Nelf-A CG5874] Negative elongation factor A
[YEF3 EFC1 TEF3 YEF3A YLR249W L9672.5] Elongation factor 3A (EF-3) (EF-3A) (EC 3.6.4.-) (Eukaryotic elongation factor 3) (eEF3) (Translation elongation factor 3A) (Yeast elongation factor 3)
[Nelfe Apom Bat4 C2 C4a Cfb Csnk2b G7e Hspa1a Hspa1b Hspa1l Ly6g5c Ly6g6c Neu1 Ng35 Rdbp Rps25-ps2 Stk19 rCG_38266] Negative elongation factor E
[TEF1 YPR080W P9513.7; TEF2 YBR118W YBR0913] Elongation factor 1-alpha (EF-1-alpha) (Eukaryotic elongation factor 1A) (eEF1A) (Translation elongation factor 1A)
[Nelfa Whsc2 Whsc2h] Negative elongation factor A (NELF-A) (Wolf-Hirschhorn syndrome candidate 2 homolog) (mWHSC2)
[EFB1 TEF5 YAL003W] Elongation factor 1-beta (EF-1-beta) (Eukaryotic elongation factor 1Balpha) (eEF1Balpha) (Translation elongation factor 1B alpha)
[Eef1a1 Eef1a] Elongation factor 1-alpha 1 (EF-1-alpha-1) (Elongation factor Tu) (EF-Tu) (Eukaryotic elongation factor 1 A-1) (eEF1A-1)
[Eef1a1 Eef1a] Elongation factor 1-alpha 1 (EF-1-alpha-1) (Elongation factor Tu) (EF-Tu) (Eukaryotic elongation factor 1 A-1) (eEF1A-1)
[SPT4 YGR063C] Transcription elongation factor SPT4 (Chromatin elongation factor SPT4)
[SUPT5H SPT5 SPT5H] Transcription elongation factor SPT5 (hSPT5) (DRB sensitivity-inducing factor 160 kDa subunit) (DSIF p160) (DRB sensitivity-inducing factor large subunit) (DSIF large subunit) (Tat-cotransactivator 1 protein) (Tat-CT1 protein)
[EEF2K] Eukaryotic elongation factor 2 kinase (eEF-2 kinase) (eEF-2K) (EC 2.7.11.20) (Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase)
[SUPT4H1 SPT4H SUPT4H] Transcription elongation factor SPT4 (hSPT4) (DRB sensitivity-inducing factor 14 kDa subunit) (DSIF p14) (DRB sensitivity-inducing factor small subunit) (DSIF small subunit)
[SPT5 YML010W YM9571.08] Transcription elongation factor SPT5 (Chromatin elongation factor SPT5)
[Eef2k] Eukaryotic elongation factor 2 kinase (eEF-2 kinase) (eEF-2K) (EC 2.7.11.20) (Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase)
[Eef2k] Eukaryotic elongation factor 2 kinase (eEF-2 kinase) (eEF-2K) (EC 2.7.11.20) (Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase)
[SPT6 CRE2 SSN20 YGR116W G6169] Transcription elongation factor SPT6 (Chromatin elongation factor SPT6)
[TUFA RAB8D RABE1B At4g20360 F9F13.10] Elongation factor Tu, chloroplastic (EF-Tu) (Ras-related protein RABE1b) (AtRABE1b) (Ras-related protein Rab8D) (AtRab8D)
[EEF1D EF1D] Elongation factor 1-delta (EF-1-delta) (Antigen NY-CO-4)
[spt6 SPAC1F7.01c SPAC694.07c] Transcription elongation factor spt6 (Chromatin elongation factor spt6)
[Spt5 CG7626] Transcription elongation factor SPT5 (DRB sensitivity-inducing factor large subunit) (DSIF large subunit) (dSpt5)
[supt5h fog spt5] Transcription elongation factor SPT5 (DRB sensitivity-inducing factor large subunit) (DSIF large subunit) (Protein foggy)
[TCERG1 CA150 TAF2S] Transcription elongation regulator 1 (TATA box-binding protein-associated factor 2S) (Transcription factor CA150)

Bibliography :
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