GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Non-homologous end joining protein Ku

 KU_PSEAE                Reviewed;         293 AA.
Q9I1W5;
19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
01-MAR-2001, sequence version 1.
23-FEB-2022, entry version 98.
RecName: Full=Non-homologous end joining protein Ku {ECO:0000255|HAMAP-Rule:MF_01875};
Name=ku {ECO:0000255|HAMAP-Rule:MF_01875}; OrderedLocusNames=PA2150;
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
14847 / LMG 12228 / 1C / PRS 101 / PAO1).
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
NCBI_TaxID=208964;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
PRS 101 / PAO1;
PubMed=10984043; DOI=10.1038/35023079;
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
pathogen.";
Nature 406:959-964(2000).
[2]
FUNCTION, SUBUNIT, AND DNA-BINDING.
PubMed=20018881; DOI=10.1074/jbc.m109.073874;
Zhu H., Shuman S.;
"Gap filling activities of Pseudomonas DNA ligase D (LigD) polymerase and
functional interactions of LigD with the DNA end-binding Ku protein.";
J. Biol. Chem. 285:4815-4825(2010).
-!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
repair enzyme, which repairs dsDNA breaks with reduced fidelity.
Stimulates rNTP addition to DSB and end joining (ligation) of linear
DNA by LigD, on 3'-overhangs and probably also 5'-overhangs and blunt
dsDNA breaks. Binds both ends of linear dsDNA protecting it from
exonuclease activity. {ECO:0000269|PubMed:20018881}.
-!- SUBUNIT: Homodimer, may form higher-order multimers on DNA. Non-
dimerized protein does not stimulate LigD ligase activity. Probably
interacts with LigD. {ECO:0000269|PubMed:20018881}.
-!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
Rule:MF_01875}.
---------------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
---------------------------------------------------------------------------
EMBL; AE004091; AAG05538.1; -; Genomic_DNA.
PIR; E83376; E83376.
RefSeq; NP_250840.1; NC_002516.2.
RefSeq; WP_003113644.1; NZ_QZGE01000014.1.
SMR; Q9I1W5; -.
STRING; 287.DR97_6276; -.
PaxDb; Q9I1W5; -.
EnsemblBacteria; AAG05538; AAG05538; PA2150.
GeneID; 881375; -.
KEGG; pae:PA2150; -.
PATRIC; fig|208964.12.peg.2249; -.
PseudoCAP; PA2150; -.
HOGENOM; CLU_048975_2_0_6; -.
InParanoid; Q9I1W5; -.
OMA; IRYRKVC; -.
PhylomeDB; Q9I1W5; -.
Proteomes; UP000002438; Chromosome.
GO; GO:0003690; F:double-stranded DNA binding; IDA:UniProtKB.
GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
GO; GO:0051351; P:positive regulation of ligase activity; IDA:UniProtKB.
CDD; cd00789; KU_like; 1.
Gene3D; 2.40.290.10; -; 1.
HAMAP; MF_01875; Prokaryotic_Ku; 1.
InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
InterPro; IPR009187; Prok_Ku.
InterPro; IPR016194; SPOC-like_C_dom_sf.
PANTHER; PTHR41251; PTHR41251; 1.
Pfam; PF02735; Ku; 1.
PIRSF; PIRSF006493; Prok_Ku; 1.
SMART; SM00559; Ku78; 1.
SUPFAM; SSF100939; SSF100939; 1.
TIGRFAMs; TIGR02772; Ku_bact; 1.
1: Evidence at protein level;
DNA damage; DNA recombination; DNA repair; DNA-binding; Reference proteome.
CHAIN 1..293
/note="Non-homologous end joining protein Ku"
/id="PRO_0000425946"
DOMAIN 10..195
/note="Ku"
/evidence="ECO:0000255|HAMAP-Rule:MF_01875"
REGION 216..229
/note="Required for dimerization"
REGION 260..293
/note="Disordered"
/evidence="ECO:0000256|SAM:MobiDB-lite"
COMPBIAS 260..279
/note="Basic and acidic residues"
/evidence="ECO:0000256|SAM:MobiDB-lite"
SEQUENCE 293 AA; 32790 MW; 042CD9E32F8A8041 CRC64;
MARAIWKGAI SFGLVHIPVS LSAATSSQGI DFDWLDQRSM EPVGYKRVNK VTGKEIEREN
IVKGVEYEKG RYVVLSEEEI RAAHPKSTQT IEIFAFVDSQ EIPLQHFDTP YYLVPDRRGG
KVYALLRETL ERTGKVALAN VVLHTRQHLA LLRPLQDALV LITLRWPSQV RSLDGLELDE
SVTEAKLDKR ELEMAKRLVE DMASHWEPDE YKDSFSDKIM KLVEEKAAKG QLHAVEEEEE
VAGKGADIID LTDLLKRSLR SRAGGGKDKG SEKAGADAKG RAKSGASRSR RKA


Related products :

Catalog number Product name Quantity
EIAAB27145 Mouse,Mus musculus,Nhej1,Non-homologous end-joining factor 1,Protein cernunnos,Xlf,XRCC4-like factor
EIAAB27147 Nhej1,Non-homologous end-joining factor 1,Protein cernunnos,Rat,Rattus norvegicus,Xlf,XRCC4-like factor
EIAAB27146 Homo sapiens,Human,NHEJ1,Non-homologous end-joining factor 1,Protein cernunnos,XLF,XRCC4-like factor
CSB-EL015787RA Rat Non-homologous end-joining factor 1(NHEJ1) ELISA kit 96T
CSB-EL015787RA Rat Non-homologous end-joining factor 1(NHEJ1) ELISA kit SpeciesRat 96T
CSB-EL015787HU Human Non-homologous end-joining factor 1(NHEJ1) ELISA kit 96T
CSB-EL015787MO Mouse Non-homologous end-joining factor 1(NHEJ1) ELISA kit 96T
NHEJ1_RAT ELISA Kit FOR Non-homologous end-joining factor 1; organism: Rat; gene name: Nhej1 96T
CSB-EL015787MO Mouse Non-homologous end-joining factor 1(NHEJ1) ELISA kit SpeciesMouse 96T
CSB-EL015787HU Human Non-homologous end-joining factor 1(NHEJ1) ELISA kit SpeciesHuman 96T
NHEJ1_MOUSE ELISA Kit FOR Non-homologous end-joining factor 1; organism: Mouse; gene name: Nhej1 96T
EIAAB10727 Bos taurus,Bovine,C_EBP-homologous protein,C_EBP-homologous protein 10,CHOP,CHOP,CHOP10,CHOP-10,DDIT3,DDIT-3,DNA damage-inducible transcript 3 protein,GADD153,Growth arrest and DNA-damage-inducible pr
EIAAB10726 C_EBP-homologous protein,C_EBP-homologous protein 10,CHOP,Chop,Chop10,CHOP-10,Ddit3,DDIT-3,DNA damage-inducible transcript 3 protein,Gadd153,Growth arrest and DNA-damage-inducible protein GADD153,Mous
EIAAB10729 C_EBP-homologous protein,C_EBP-homologous protein 10,CHOP,CHOP,CHOP10,CHOP-10,DDIT3,DDIT-3,DNA damage-inducible transcript 3 protein,GADD153,Growth arrest and DNA damage-inducible protein GADD153,Homo
EIAAB10728 C_EBP-homologous protein,C_EBP-homologous protein 10,CHOP,Chop,Chop10,CHOP-10,Ddit3,DDIT-3,DNA damage-inducible transcript 3 protein,Gadd153,Growth arrest and DNA-damage-inducible protein GADD153,Rat,
26-275 REC8 is required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chrom 0.05 mg
29-863 NSMCE1 is a probable component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination b 0.1 mg
E1094h Rat ELISA Kit FOR Calcineurin B homologous protein 2 96T
EIAAB46352 Homo sapiens,Human,PP10631,WAS_WASL-interacting protein family member 2,WASP-interacting protein-related protein,WICH,WIP- and CR16-homologous protein,WIPF2,WIP-related protein,WIRE
E1485h Mouse ELISA Kit FOR Calcineurin B homologous protein 2 96T
29-005 BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine_threonine kinase. Each of these proteins contains two bromodom 0.05 mg
18-661-15196 Bcl-2 homologous antagonist_killer - Apoptosis regulator BAK; Bcl-2-like 7 protein Polyclonal 0.1 mg
18-003-42308 Forkhead box protein J2 - Fork head homologous X Polyclonal 0.1 mg Protein A
18-003-43834 Forkhead box protein J2 - Fork head homologous X Polyclonal 0.1 mg Protein A
CSB-EL018892RA Rat Homologous-pairing protein 2 homolog(PSMC3IP) ELISA kit 96T
Pathways :
WP1222: Non-homologous end joining
WP1324: Non-homologous end joining
WP438: Non-homologous end joining
WP1201: Non-homologous end joining
WP1676: Non-homologous end-joining
WP753: Non-homologous end joining
WP1663: Homologous recombination
WP739: Non-homologous end joining
WP1097: Non-homologous end joining
WP1242: Non-homologous end joining
WP861: Non-homologous end joining
WP979: Non-homologous end joining
WP1208: Non-homologous end joining
WP1570: Non-homologous end joining
WP1277: Non-homologous end joining
WP1036: Homologous recombination
WP1657: Glycerolipid metabolism
WP232: G Protein Signaling Pathways
WP1362: Homologous recombination
WP1700: Selenoamino acid metabolism
WP931: G Protein Signaling Pathways
WP1493: Carbon assimilation C4 pathway
WP186: Homologous recombination
WP1153: Homologous recombination
WP346: Protein Modifications

Related Genes :
[NHEJ1 XLF] Non-homologous end-joining factor 1 (Protein cernunnos) (XRCC4-like factor)
[Nhej1 Xlf] Non-homologous end-joining factor 1 (Protein cernunnos) (XRCC4-like factor)
[ligD Rv0938 MTCY08D9.01c MTCY10D7.36c] Multifunctional non-homologous end joining DNA repair protein LigD (NHEJ DNA repair protein D) (Mt-Lig) (NHEJ DNA polymerase) [Includes: DNA repair polymerase (Pol) (Polymerase/primase); 3'-phosphoesterase (3'-ribonuclease/3'-phosphatase) (PE); DNA ligase (Lig) (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP])]
[ligD MSMEG_5570 MSMEI_5419] Multifunctional non-homologous end joining protein LigD (NHEJ DNA polymerase) [Includes: DNA repair polymerase (Pol) (Polymerase/primase); 3'-phosphoesterase (3'-ribonuclease/3'-phosphatase) (PE); DNA ligase (Lig) (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP])]
[CYREN C7orf49 MRI] Cell cycle regulator of non-homologous end joining (Cell cycle regulator of NHEJ) (Modulator of retrovirus infection homolog)
[ligD PA2138] Multifunctional non-homologous end joining protein LigD (NHEJ DNA polymerase) [Includes: 3'-phosphoesterase (3'-ribonuclease/3'-phosphatase); DNA ligase D (LigD) (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP]); DNA repair polymerase (Pol) (Polymerase/primase)]
[NEJ1 LIF2 YLR265C] Non-homologous end-joining protein 1
[Cyren Mri] Cell cycle regulator of non-homologous end joining (Cell cycle regulator of NHEJ) (Modulator of retrovirus infection homolog)
[ligd ykoU BSU13400] Bifunctional non-homologous end joining protein LigD (NHEJ DNA polymerase) [Includes: DNA ligase (Lig) (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP]); DNA repair polymerase (Pol) (Polymerase/primase)]
[mku Rv0937c] Non-homologous end joining protein Ku (Mt-Ku)
[IFFO1 IFFO] Non-homologous end joining factor IFFO1 (NHEJ factor IFFO1) (Intermediate filament family orphan 1) (Tumor antigen HOM-TES-103)
[ligD BQ2027_MB0963] Multifunctional non-homologous end joining protein LigD (NHEJ DNA polymerase) [Includes: DNA repair polymerase (Pol) (Polymerase/primase); 3'-phosphoesterase (3'-ribonuclease/3'-phosphatase) (PE); DNA ligase (Lig) (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP])]
[ligD MT0965] Multifunctional non-homologous end joining DNA repair protein LigD (NHEJ DNA repair protein D) (Mt-Lig) (NHEJ DNA polymerase) [Includes: DNA repair polymerase (Pol) (Polymerase/primase); 3'-phosphoesterase (3'-ribonuclease/3'-phosphatase) (PE); DNA ligase (Lig) (EC 6.5.1.1) (Polydeoxyribonucleotide synthase [ATP])]
[ku MSMEG_5580 MSMEI_5431] Non-homologous end joining protein Ku
[ku PA2150] Non-homologous end joining protein Ku
[Nhej1 Xlf] Non-homologous end-joining factor 1 (Protein cernunnos) (XRCC4-like factor)
[ku ykoV BSU13410] Non-homologous end joining protein Ku
[XRCC6 G22P1] X-ray repair cross-complementing protein 6 (EC 3.6.4.-) (EC 4.2.99.-) (5'-deoxyribose-5-phosphate lyase Ku70) (5'-dRP lyase Ku70) (70 kDa subunit of Ku antigen) (ATP-dependent DNA helicase 2 subunit 1) (ATP-dependent DNA helicase II 70 kDa subunit) (CTC box-binding factor 75 kDa subunit) (CTC75) (CTCBF) (DNA repair protein XRCC6) (Lupus Ku autoantigen protein p70) (Ku70) (Thyroid-lupus autoantigen) (TLAA) (X-ray repair complementing defective repair in Chinese hamster cells 6)
[Iffo1 Iffo] Non-homologous end joining factor IFFO1 (NHEJ factor IFFO1) (Intermediate filament family orphan 1)
[XRCC5 G22P2] X-ray repair cross-complementing protein 5 (EC 3.6.4.-) (86 kDa subunit of Ku antigen) (ATP-dependent DNA helicase 2 subunit 2) (ATP-dependent DNA helicase II 80 kDa subunit) (CTC box-binding factor 85 kDa subunit) (CTC85) (CTCBF) (DNA repair protein XRCC5) (Ku80) (Ku86) (Lupus Ku autoantigen protein p86) (Nuclear factor IV) (Thyroid-lupus autoantigen) (TLAA) (X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining))
[Xrcc6 G22p1 Ku70] X-ray repair cross-complementing protein 6 (EC 3.6.4.-) (EC 4.2.99.-) (5'-deoxyribose-5-phosphate lyase Ku70) (5'-dRP/AP lyase Ku70) (ATP-dependent DNA helicase 2 subunit 1) (ATP-dependent DNA helicase II 70 kDa subunit) (CTC box-binding factor 75 kDa subunit) (CTC75) (CTCBF) (DNA repair protein XRCC6) (Ku autoantigen protein p70 homolog) (Ku70)
[Xrcc5 G22p2] X-ray repair cross-complementing protein 5 (EC 3.6.4.-) (ATP-dependent DNA helicase 2 subunit 2) (ATP-dependent DNA helicase II 80 kDa subunit) (CTC box-binding factor 85 kDa subunit) (CTC85) (CTCBF) (DNA repair protein XRCC5) (Ku autoantigen protein p86 homolog) (Ku80) (Nuclear factor IV)
[YKU80 HDF2 YMR106C YM9718.05C] ATP-dependent DNA helicase II subunit 2 (EC 3.6.4.12) (ATP-dependent DNA helicase II subunit Ku80) (High affinity DNA-binding factor subunit 2) (Yeast Ku80)
[XRCC6 G22P1 KU70] X-ray repair cross-complementing protein 5 (EC 3.6.4.-) (EC 4.2.99.-) (5'-deoxyribose-5-phosphate lyase Ku70) (5'-dRP/AP lyase Ku70) (ATP-dependent DNA helicase 2 subunit 1) (ATP-dependent DNA helicase II 70 kDa subunit) (DNA repair protein XRCC6) (Ku autoantigen protein p70 homolog) (Ku70)
[KU70 At1g16970 F6I1.2] ATP-dependent DNA helicase 2 subunit KU70 (EC 3.6.4.12) (ATP-dependent DNA helicase 2 subunit 1) (ATP-dependent DNA helicase II 70 kDa subunit)
[KU80 At1g48050 F21D18.26 T2J15.4] ATP-dependent DNA helicase 2 subunit KU80 (EC 3.6.4.12) (ATP-dependent DNA helicase 2 subunit 2) (ATP-dependent DNA helicase II 80 kDa subunit)
[pku70 ku70 SPCC126.02c] ATP-dependent DNA helicase II subunit 1 (EC 3.6.4.12) (ATP-dependent DNA helicase II subunit Ku70)
[YKU70 HDF1 NES24 YMR284W YM8021.10] ATP-dependent DNA helicase II subunit 1 (EC 3.6.4.12) (ATP-dependent DNA helicase II subunit Ku70) (High affinity DNA-binding factor subunit 1)
[KU70 Os07g0184900 LOC_Os07g08729 OJ1046_F10.128 OsJ_23357 OsJ_23359] ATP-dependent DNA helicase 2 subunit KU70 (OsKU70) (EC 3.6.4.12) (ATP-dependent DNA helicase 2 subunit 1) (ATP-dependent DNA helicase II 70 kDa subunit)
[pku80 ku80 SPBC543.03c] ATP-dependent DNA helicase II subunit 2 (EC 3.6.4.12) (ATP-dependent DNA helicase II subunit Ku80) (Pombe Ku80)

Bibliography :
[35560331] Examining the Interaction Between Cas9 and Ku, and its Implications for Gene Editing.
[35197472] Small Cajal body-associated RNA 2 (scaRNA2) regulates DNA repair pathway choice by inhibiting DNA-PK.
[35113100] Platinum complexes as inhibitors of DNA repair protein Ku70 and topoisomerase II╬▒ in cancer cells.
[35079021] USP44 regulates irradiation-induced DNA double-strand break repair and suppresses tumorigenesis in nasopharyngeal carcinoma.
[35036867] WASH interacts with Ku to regulate DNA double-stranded break repair.
[35027540] Linking DNA repair and cell cycle progression through serine ADP-ribosylation of histones.
[35000298] Feline XRCC4 undergoes rapid Ku-dependent recruitment to DNA damage sites.
[34987222] Structural insights into inhibitor regulation of the DNA repair protein DNA-PKcs.
[34966825] TEX10 Promotes the Tumorigenesis and Radiotherapy Resistance of Urinary Bladder Carcinoma by Stabilizing XRCC6.
[34936881] Autophosphorylation transforms DNA-PK from protecting to processing DNA ends.