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Pesticidal crystal protein Cry3Aa (73 kDa crystal protein) (Crystaline entomocidal protoxin) (Insecticidal delta-endotoxin CryIIIA(a))

 CR3AA_BACTD             Reviewed;         644 AA.
P0A381; P07130; P21255;
01-APR-1988, integrated into UniProtKB/Swiss-Prot.
01-APR-1988, sequence version 1.
07-NOV-2018, entry version 49.
RecName: Full=Pesticidal crystal protein Cry3Aa;
AltName: Full=73 kDa crystal protein;
AltName: Full=Crystaline entomocidal protoxin;
AltName: Full=Insecticidal delta-endotoxin CryIIIA(a);
Flags: Precursor;
Name=cry3Aa; Synonyms=bt13, cry3A, cryC, cryIIIa, cryIIIA(a);
Bacillus thuringiensis subsp. san diego.
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
Bacillus cereus group.
NCBI_TaxID=1435;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2828180; DOI=10.1016/0378-1119(87)90174-0;
Herrnstadt C., Gilroy T.E., Sobieski D.A., Bennett B.D.,
Gaertner F.H.;
"Nucleotide sequence and deduced amino acid sequence of a coleopteran-
active delta-endotoxin gene from Bacillus thuringiensis subsp. san
diego.";
Gene 57:37-46(1987).
-!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
epithelial cells of Coleoptera.
-!- DEVELOPMENTAL STAGE: The crystal protein is produced during
sporulation and is accumulated both as an inclusion and as part of
the spore coat.
-!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
terminus.
-!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=AAA22336.1; Type=Erroneous initiation; Evidence={ECO:0000305};
-----------------------------------------------------------------------
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Distributed under the Creative Commons Attribution (CC BY 4.0) License
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EMBL; M22472; AAA22336.1; ALT_INIT; Genomic_DNA.
ProteinModelPortal; P0A381; -.
SMR; P0A381; -.
GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
Gene3D; 1.20.190.10; -; 1.
Gene3D; 2.100.10.10; -; 1.
Gene3D; 2.60.120.260; -; 1.
InterPro; IPR008979; Galactose-bd-like_sf.
InterPro; IPR038979; Pest_crys.
InterPro; IPR005638; Pest_crys_C.
InterPro; IPR005639; Pest_crys_N.
InterPro; IPR036716; Pest_crys_N_sf.
InterPro; IPR001178; Pest_cryst_cen_dom.
InterPro; IPR036399; Pest_cryst_cen_dom_sf.
PANTHER; PTHR37003; PTHR37003; 1.
Pfam; PF03944; Endotoxin_C; 1.
Pfam; PF00555; Endotoxin_M; 1.
Pfam; PF03945; Endotoxin_N; 1.
SUPFAM; SSF49785; SSF49785; 1.
SUPFAM; SSF51096; SSF51096; 1.
SUPFAM; SSF56849; SSF56849; 1.
2: Evidence at transcript level;
Sporulation; Toxin.
PROPEP 1 57 Removed in mature form.
/FTId=PRO_0000006341.
CHAIN 58 644 Pesticidal crystal protein Cry3Aa.
/FTId=PRO_0000006342.
SEQUENCE 644 AA; 73109 MW; EFCFF1CEEFDA9683 CRC64;
MNPNNRSEHD TIKTTENNEV PTNHVQYPLA ETPNPTLEDL NYKEFLRMTA DNNTEALDSS
TTKDVIQKGI SVVGDLLGVV GFPFGGALVS FYTNFLNTIW PSEDPWKAFM EQVEALMDQK
IADYAKNKAL AELQGLQNNV EDYVSALSSW QKNPVSSRNP HSQGRIRELF SQAESHFRNS
MPSFAISGYE VLFLTTYAQA ANTHLFLLKD AQIYGEEWGY EKEDIAEFYK RQLKLTQEYT
DHCVKWYNVG LDKLRGSSYE SWVNFNRYRR EMTLTVLDLI ALFPLYDVRL YPKEVKTELT
RDVLTDPIVG VNNLRGYGTT FSNIENYIRK PHLFDYLHRI QFHTRFQPGY YGNDSFNYWS
GNYVSTRPSI GSNDIITSPF YGNKSSEPVQ NLEFNGEKVY RAVANTNLAV WPSAVYSGVT
KVEFSQYNDQ TDEASTQTYD SKRNVGAVSW DSIDQLPPET TDEPLEKGYS HQLNYVMCFL
MQGSRGTIPV LTWTHKSVDF FNMIDSKKIT QLPLVKAYKL QSGASVVAGP RFTGGDIIQC
TENGSAATIY VTPDVSYSQK YRARIHYAST SQITFTLSLD GAPFNQYYFD KTINKGDTLT
YNSFNLASFS TPFELSGNNL QIGVTGLSAG DKVYIDKIEF IPVN


Related products :

Catalog number Product name Quantity
abx109065 Polyclonal Rabbit Pesticidal crystal protein cry3Aa Antibody (HRP) 100 μg
abx107648 Polyclonal Rabbit Pesticidal crystal protein cry3Aa Antibody (FITC) 100 μg
abx106234 Polyclonal Rabbit Pesticidal crystal protein cry3Aa Antibody (Biotin) 100 μg
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abx109060 Polyclonal Rabbit Pesticidal crystal protein cry1Ab Antibody (HRP) 100 μg
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abx109064 Polyclonal Rabbit Pesticidal crystal protein cry1Fb Antibody (HRP) 100 μg
abx109063 Polyclonal Rabbit Pesticidal crystal protein cry1Ac Antibody (HRP) 100 μg
abx109062 Polyclonal Rabbit Pesticidal crystal protein cry1Ab Antibody (HRP) 100 μg
abx107643 Polyclonal Rabbit Pesticidal crystal protein cry1Ab Antibody (FITC) 100 μg
abx106230 Polyclonal Rabbit Pesticidal crystal protein cry1Ac Antibody (Biotin) 100 μg
abx106231 Polyclonal Rabbit Pesticidal crystal protein cry1Ab Antibody (Biotin) 100 μg
abx106229 Polyclonal Rabbit Pesticidal crystal protein cry1Ab Antibody (Biotin) 100 μg
abx107644 Polyclonal Rabbit Pesticidal crystal protein cry1Ac Antibody (FITC) 100 μg
abx107645 Polyclonal Rabbit Pesticidal crystal protein cry1Ab Antibody (FITC) 100 μg
abx107647 Polyclonal Rabbit Pesticidal crystal protein cry1Fb Antibody (FITC) 100 μg
abx106232 Polyclonal Rabbit Pesticidal crystal protein cry1Ac Antibody (Biotin) 100 μg
abx106233 Polyclonal Rabbit Pesticidal crystal protein cry1Fb Antibody (Biotin) 100 μg
abx107646 Polyclonal Rabbit Pesticidal crystal protein cry1Ac Antibody (FITC) 100 μg
MSK-2 Crystal Harvesting Sampler Kit, contains 100 mounts for crystal harvesting and data collection; 20 Dual Thickness MicroMounts, 20 MicroLoops LD, 20 MicroMesh, 20 MicroLoop E, and 20 MicroGrippers kit
MSK-2 Crystal Harvesting Sampler Kit, contains 100 mounts for crystal harvesting and data collection; 20 Dual Thickness MicroMounts, 20 MicroLoops LD, 20 MicroMesh, 20 MicroLoop E, and 20 MicroGrippers 1 kit
MSK-2 Crystal Harvesting Sampler Kit, contains 100 mounts for crystal harvesting and data collection; 20 Dual Thickness MicroMounts, 20 MicroLoops LD, 20 MicroMesh, 20 MicroLoop E, and 20 MicroGrippers kit
MSK-1 Crystal Harvesting Sampler Kit, contains 40 mounts for crystal harvesting and data collection; 10 Dual Thickness MicroMounts, 10 MicroLoops, 5 MicroMesh, 5 MicroLoop LD, 5 MicroLoop E, and 5 MicroGrip 1 kit
MSK-1 Crystal Harvesting Sampler Kit, contains 40 mounts for crystal harvesting and data collection; 10 Dual Thickness MicroMounts, 10 MicroLoops, 5 MicroMesh, 5 MicroLoop LD, 5 MicroLoop E, and 5 MicroGrip kit
orb60731 Crystal violet Crystal violet is a triarylmethane dye. For research use only. 10 mg

Kits Elisa; taq POLYMERASE

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Pathways :
WP1644: DNA replication
WP1663: Homologous recombination
WP1672: Mismatch repair
WP1693: Purine metabolism
WP1694: Pyrimidine metabolism
WP1049: G Protein Signaling Pathways
WP1165: G Protein Signaling Pathways
WP1371: G Protein Signaling Pathways
WP1438: Influenza A virus infection
WP1493: Carbon assimilation C4 pathway
WP1502: Mitochondrial biogenesis
WP1531: Vitamin D synthesis
WP1566: Citrate cycle (TCA cycle)
WP1613: 1,4-Dichlorobenzene degradation
WP1616: ABC transporters
WP1624: Bacterial secretion system
WP1625: Base excision repair
WP1650: Fluorobenzoate degradation
WP1654: gamma-Hexachlorocyclohexane degradation
WP1657: Glycerolipid metabolism
WP1659: Glycine, serine and threonine metabolism
WP1661: Glyoxylate and dicarboxylate metabolism
WP1665: Limonene and pinene degradation
WP1673: Naphthalene and anthracene degradation
WP1675: Nitrogen metabolism

Related Genes :
[cry6Aa cryVIa cryVIA(a)] Pesticidal crystal protein Cry6Aa (Crystaline entomocidal protoxin) (Crystal protein) (Insecticidal delta-endotoxin CryVIA(a)) (Fragment)
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[LTF GIG12 LF] Lactotransferrin (Lactoferrin) (EC 3.4.21.-) (Growth-inhibiting protein 12) (Talalactoferrin) [Cleaved into: Lactoferricin-H (Lfcin-H); Kaliocin-1; Lactoferroxin-A; Lactoferroxin-B; Lactoferroxin-C]
[NUDT21 CFIM25 CPSF25 CPSF5] Cleavage and polyadenylation specificity factor subunit 5 (Cleavage and polyadenylation specificity factor 25 kDa subunit) (CPSF 25 kDa subunit) (Cleavage factor Im complex 25 kDa subunit) (CFIm25) (Nucleoside diphosphate-linked moiety X motif 21) (Nudix motif 21) (Nudix hydrolase 21) (Pre-mRNA cleavage factor Im 68 kDa subunit)
[RTS1 SCS1 YOR014W OR26.04] Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (PP2A, B subunit, B' delta isoform) (Protein RTS1) (Protein SCS1)
[PPP2R5D] Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (PP2A B subunit isoform B'-delta) (PP2A B subunit isoform B56-delta) (PP2A B subunit isoform PR61-delta) (PP2A B subunit isoform R5-delta)
[HSPA1A HSP72 HSPA1 HSX70] Heat shock 70 kDa protein 1A (Heat shock 70 kDa protein 1) (HSP70-1) (HSP70.1)
[HSPA1B HSP72] Heat shock 70 kDa protein 1B (Heat shock 70 kDa protein 2) (HSP70-2) (HSP70.2)
[AKR1D1 SRD5B1] 3-oxo-5-beta-steroid 4-dehydrogenase (EC 1.3.1.3) (Aldo-keto reductase family 1 member D1) (Delta(4)-3-ketosteroid 5-beta-reductase) (Delta(4)-3-oxosteroid 5-beta-reductase)
[Prkcd Pkcd] Protein kinase C delta type (EC 2.7.11.13) (Tyrosine-protein kinase PRKCD) (EC 2.7.10.2) (nPKC-delta) [Cleaved into: Protein kinase C delta type regulatory subunit; Protein kinase C delta type catalytic subunit (Sphingosine-dependent protein kinase-1) (SDK1)]
[Prkcd Pkcd] Protein kinase C delta type (EC 2.7.11.13) (nPKC-delta) [Cleaved into: Protein kinase C delta type regulatory subunit; Protein kinase C delta type catalytic subunit (Sphingosine-dependent protein kinase-1) (SDK1)]
[KMT2A ALL1 CXXC7 HRX HTRX MLL MLL1 TRX1] Histone-lysine N-methyltransferase 2A (Lysine N-methyltransferase 2A) (EC 2.1.1.43) (ALL-1) (CXXC-type zinc finger protein 7) (Myeloid/lymphoid or mixed-lineage leukemia) (Myeloid/lymphoid or mixed-lineage leukemia protein 1) (Trithorax-like protein) (Zinc finger protein HRX) [Cleaved into: MLL cleavage product N320 (N-terminal cleavage product of 320 kDa) (p320); MLL cleavage product C180 (C-terminal cleavage product of 180 kDa) (p180)]
[cardA] Procardosin-A (EC 3.4.23.-) [Cleaved into: Cardosin-A intermediate form 35 kDa subunit; Cardosin-A heavy chain (Cardosin-A 31 kDa subunit); Cardosin-A intermediate form 30 kDa subunit; Cardosin-A light chain (Cardosin-A 15 kDa subunit)]
[ADAR ADAR1 DSRAD G1P1 IFI4] Double-stranded RNA-specific adenosine deaminase (DRADA) (EC 3.5.4.37) (136 kDa double-stranded RNA-binding protein) (p136) (Interferon-inducible protein 4) (IFI-4) (K88DSRBP)
[BECN1 GT197] Beclin-1 (Coiled-coil myosin-like BCL2-interacting protein) (Protein GT197) [Cleaved into: Beclin-1-C 35 kDa; Beclin-1-C 37 kDa]
[] Genome polyprotein [Cleaved into: Core protein p21 (Capsid protein C) (p21); Core protein p19; Envelope glycoprotein E1 (gp32) (gp35); Envelope glycoprotein E2 (NS1) (gp68) (gp70); p7; Protease NS2-3 (p23) (EC 3.4.22.-); Serine protease NS3 (EC 3.4.21.98) (EC 3.6.1.15) (EC 3.6.4.13) (Hepacivirin) (NS3P) (p70); Non-structural protein 4A (NS4A) (p8); Non-structural protein 4B (NS4B) (p27); Non-structural protein 5A (NS5A) (p56); RNA-directed RNA polymerase (EC 2.7.7.48) (NS5B) (p68)]
[GP] Envelope glycoprotein (GP1,2) (GP) [Cleaved into: GP1; GP2; GP2-delta]
[] Genome polyprotein [Cleaved into: P1 proteinase (EC 3.4.-.-) (N-terminal protein); Helper component proteinase (HC-pro) (EC 3.4.22.45); Protein P3; 6 kDa protein 1 (6K1); Cytoplasmic inclusion protein (CI) (EC 3.6.4.-); 6 kDa protein 2 (6K2); Viral genome-linked protein (VPg); Nuclear inclusion protein A (NI-a) (NIa) (EC 3.4.22.44) (49 kDa proteinase) (49 kDa-Pro) (NIa-pro); Nuclear inclusion protein B (NI-b) (NIb) (EC 2.7.7.48) (RNA-directed RNA polymerase); Capsid protein (CP) (Coat protein)]
[pstS1 phoS1 Rv0934 MTCY08D9.05c] Phosphate-binding protein PstS 1 (PBP 1) (PhoS1) (PstS-1) (38-kDa glycolipoprotein) (38-kDa lipoprotein) (P38) (Antigen Ag78) (Protein antigen B) (Pab)
[HSD3B1 3BH HSDB3A] 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I) (3-beta-HSD I) (3-beta-hydroxy-5-ene steroid dehydrogenase) (3-beta-hydroxy-Delta(5)-steroid dehydrogenase) (EC 1.1.1.145) (3-beta-hydroxysteroid 3-dehydrogenase) (EC 1.1.1.270) (Delta-5-3-ketosteroid isomerase) (Dihydrotestosterone oxidoreductase) (EC 1.1.1.210) (Steroid Delta-isomerase) (EC 5.3.3.1) (Trophoblast antigen FDO161G)
[F8 F8C] Coagulation factor VIII (Antihemophilic factor) (AHF) (Procoagulant component) [Cleaved into: Factor VIIIa heavy chain, 200 kDa isoform; Factor VIIIa heavy chain, 92 kDa isoform; Factor VIII B chain; Factor VIIIa light chain]
[RPSA LAMBR LAMR1] 40S ribosomal protein SA (37 kDa laminin receptor precursor) (37LRP) (37/67 kDa laminin receptor) (LRP/LR) (67 kDa laminin receptor) (67LR) (Colon carcinoma laminin-binding protein) (Laminin receptor 1) (LamR) (Laminin-binding protein precursor p40) (LBP/p40) (Multidrug resistance-associated protein MGr1-Ag) (NEM/1CHD4) (Small ribosomal subunit protein uS2)
[HSP90AA1 HSP90A HSPC1 HSPCA] Heat shock protein HSP 90-alpha (Heat shock 86 kDa) (HSP 86) (HSP86) (Lipopolysaccharide-associated protein 2) (LAP-2) (LPS-associated protein 2) (Renal carcinoma antigen NY-REN-38)
[MMP9 CLG4B] Matrix metalloproteinase-9 (MMP-9) (EC 3.4.24.35) (92 kDa gelatinase) (92 kDa type IV collagenase) (Gelatinase B) (GELB) [Cleaved into: 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9]
[Agrn Agrin] Agrin [Cleaved into: Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment (C90); Agrin C-terminal 22 kDa fragment (C22)]
[Hsd3b1 Hsd3b] 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I) (3-beta-HSD I) (3-beta-hydroxy-5-ene steroid dehydrogenase) (3-beta-hydroxy-Delta(5)-steroid dehydrogenase) (EC 1.1.1.145) (3-beta-hydroxysteroid 3-dehydrogenase) (EC 1.1.1.270) (Delta-5-3-ketosteroid isomerase) (Dihydrotestosterone oxidoreductase) (EC 1.1.1.210) (Steroid Delta-isomerase) (EC 5.3.3.1)
[HBD] Hemoglobin subunit delta (Delta-globin) (Hemoglobin delta chain)
[ISG15 G1P2 UCRP] Ubiquitin-like protein ISG15 (Interferon-induced 15 kDa protein) (Interferon-induced 17 kDa protein) (IP17) (Ubiquitin cross-reactive protein) (hUCRP)
[MAPK13 PRKM13 SAPK4] Mitogen-activated protein kinase 13 (MAP kinase 13) (MAPK 13) (EC 2.7.11.24) (Mitogen-activated protein kinase p38 delta) (MAP kinase p38 delta) (Stress-activated protein kinase 4)
[CPSF6 CFIM68] Cleavage and polyadenylation specificity factor subunit 6 (Cleavage and polyadenylation specificity factor 68 kDa subunit) (CPSF 68 kDa subunit) (Cleavage factor Im complex 68 kDa subunit) (CFIm68) (Pre-mRNA cleavage factor Im 68 kDa subunit) (Protein HPBRII-4/7)

Bibliography :