Receptor of activated protein C kinase 1 (Guanine nucleotide-binding protein subunit beta-2-like 1) (Receptor for activated C kinase) (Receptor of activated protein kinase C 1) [Cleaved into: Receptor of activated protein C kinase 1, N-terminally processed]

 RACK1_RAT               Reviewed;         317 AA.
 P63245; P25388; P99049;
 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
 23-JAN-2007, sequence version 3.
 23-FEB-2022, entry version 143.
 RecName: Full=Receptor of activated protein C kinase 1;
 AltName: Full=Guanine nucleotide-binding protein subunit beta-2-like 1;
 AltName: Full=Receptor for activated C kinase;
 AltName: Full=Receptor of activated protein kinase C 1;
 Contains:
   RecName: Full=Receptor of activated protein C kinase 1, N-terminally processed;
 Name=Rack1 {ECO:0000312|RGD:69229}; Synonyms=Gnb2l1;
 Rattus norvegicus (Rat).
 Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
 Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
 Murinae; Rattus.
 NCBI_TaxID=10116;
 [1]
 NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
 STRAIN=Sprague-Dawley; TISSUE=Brain;
 PubMed=8302854; DOI=10.1073/pnas.91.3.839;
 Ron D., Chen C.-H., Caldwell J., Jamieson L., Orr E., Mochly-Rosen D.;
 "Cloning of an intracellular receptor for protein kinase C: a homolog of
 the beta subunit of G proteins.";
 Proc. Natl. Acad. Sci. U.S.A. 91:839-843(1994).
 [2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
 TISSUE=Pituitary;
 PubMed=15489334; DOI=10.1101/gr.2596504;
 The MGC Project Team;
 "The status, quality, and expansion of the NIH full-length cDNA project:
 the Mammalian Gene Collection (MGC).";
 Genome Res. 14:2121-2127(2004).
 [3]
 PROTEIN SEQUENCE OF 45-57; 89-100; 107-118; 258-280 AND 309-317, AND
 IDENTIFICATION BY MASS SPECTROMETRY.
 STRAIN=Sprague-Dawley; TISSUE=Hippocampus;
 PubMed=17569602; DOI=10.1016/j.bbapap.2007.05.002;
 Diao W.F., Hoger H., Chen W.Q., Pollak A., Lubec G.;
 "Hippocampal signaling protein levels are different in early and late
 metestrus in the rat.";
 Biochim. Biophys. Acta 1774:1044-1051(2007).
 [4]
 FUNCTION, AND SUBUNIT.
 PubMed=15340087; DOI=10.1128/mcb.24.18.8276-8287.2004;
 Gerbasi V.R., Weaver C.M., Hill S., Friedman D.B., Link A.J.;
 "Yeast Asc1p and mammalian RACK1 are functionally orthologous core 40S
 ribosomal proteins that repress gene expression.";
 Mol. Cell. Biol. 24:8276-8287(2004).
 -!- FUNCTION: Scaffolding protein involved in the recruitment, assembly
     and/or regulation of a variety of signaling molecules. Interacts with a
     wide variety of proteins and plays a role in many cellular processes.
     Component of the 40S ribosomal subunit involved in translational
     repression (PubMed:15340087). Involved in the initiation of the
     ribosome quality control (RQC), a pathway that takes place when a
     ribosome has stalled during translation, by promoting ubiquitination of
     a subset of 40S ribosomal subunits (By similarity). Binds to and
     stabilizes activated protein kinase C (PKC), increasing PKC-mediated
     phosphorylation. May recruit activated PKC to the ribosome, leading to
     phosphorylation of EIF6. Inhibits the activity of SRC kinases including
     SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase
     of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and
     inhibits transcriptional activity of the BMAL1-CLOCK heterodimer.
     Facilitates ligand-independent nuclear translocation of AR following
     PKC activation, represses AR transactivation activity and is required
     for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin
     signaling and promotes cell spreading and contact with the
     extracellular matrix. Involved in PKC-dependent translocation of ADAM12
     to the cell membrane. Promotes the ubiquitination and proteasome-
     mediated degradation of proteins such as CLEC1B and HIF1A. Required for
     VANGL2 membrane localization, inhibits Wnt signaling, and regulates
     cellular polarization and oriented cell division during gastrulation.
     Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates
     internalization of the muscarinic receptor CHRM2. Promotes apoptosis by
     increasing oligomerization of BAX and disrupting the interaction of BAX
     with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity.
     Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a
     role in regulation of FLT1-mediated cell migration. Involved in the
     transport of ABCB4 from the Golgi to the apical bile canalicular
     membrane. {ECO:0000250|UniProtKB:P63244, ECO:0000269|PubMed:15340087,
     ECO:0000269|PubMed:8302854}.
 -!- SUBUNIT: Monomer; also forms homodimers and homooligomers (By
     similarity). Interacts with CPNE3 (By similarity). May interact with
     ABCB4 (By similarity) Component of the small (40S) ribosomal subunit
     (PubMed:15340087). Interacts with LARP4. Interacts with LARP4B.
     Interacts with PKD2L1 (By similarity). Binds SLC9A3R1. Forms a ternary
     complex with TRIM63 and PRKCE. Interacts with HABP4, KRT1 and OTUB1.
     Interacts with SRC (via SH2 domain); the interaction is enhanced by
     tyrosine phosphorylation of RACK1. Recruited in a circadian manner into
     a nuclear complex which also includes BMAL1 and PRKCA. Interacts with
     AR. Interacts with IGF1R but not with INSR. Interacts with ADAM12.
     Interacts with CLEC1B (via N-terminal region) and with HIF1A; the
     interaction promotes their degradation. Interacts with RHOA; this
     enhances RHOA activation and promotes cell migration. Interacts with
     CHRM2; the interaction regulates CHRM2 internalization. Interacts with
     TRPM6 (via kinase domain). Interacts with PTK2/FAK1; required for
     PTK2/FAK1 phosphorylation and dephosphorylation. Interacts with FLT1.
     Interacts with HRAS. {ECO:0000250|UniProtKB:P63244,
     ECO:0000269|PubMed:15340087}.
 -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P63244};
     Peripheral membrane protein {ECO:0000250|UniProtKB:P63244}. Cytoplasm
     {ECO:0000250|UniProtKB:P63244}. Cytoplasm, perinuclear region
     {ECO:0000250|UniProtKB:P63244}. Nucleus {ECO:0000250|UniProtKB:P63244}.
     Perikaryon {ECO:0000250|UniProtKB:P68040}. Cell projection, dendrite
     {ECO:0000250|UniProtKB:P68040}. Note=Recruited to the plasma membrane
     through interaction with KRT1 which binds to membrane-bound ITGB1. Also
     associated with the membrane in oncogene-transformed cells. PKC
     activation induces translocation from the perinuclear region to the
     cell periphery (By similarity). In the brain, detected mainly in cell
     bodies and dendrites with little expression in axonal fibers or nuclei
     (By similarity). {ECO:0000250|UniProtKB:P63244,
     ECO:0000250|UniProtKB:P68040}.
 -!- DOMAIN: The 7 WD repeats mediate protein-protein interactions with
     binding partners. {ECO:0000250|UniProtKB:P63244}.
 -!- PTM: Phosphorylated on Tyr-228 and/or Tyr-246 by SRC. This is required
     for binding to SRC (By similarity). {ECO:0000250}.
 -!- SIMILARITY: Belongs to the WD repeat G protein beta family. Ribosomal
     protein RACK1 subfamily. {ECO:0000305}.
 ---------------------------------------------------------------------------
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 EMBL; U03390; AAA18951.1; -; mRNA.
 EMBL; BC063809; AAH63809.1; -; mRNA.
 PIR; A36986; A36986.
 RefSeq; NP_570090.2; NM_130734.2.
 SMR; P63245; -.
 BioGRID; 249704; 6.
 CORUM; P63245; -.
 IntAct; P63245; 4.
 MINT; P63245; -.
 STRING; 10116.ENSRNOP00000003334; -.
 iPTMnet; P63245; -.
 PhosphoSitePlus; P63245; -.
 jPOST; P63245; -.
 PaxDb; P63245; -.
 PRIDE; P63245; -.
 Ensembl; ENSRNOT00000072179; ENSRNOP00000068758; ENSRNOG00000049070.
 Ensembl; ENSRNOT00000084232; ENSRNOP00000074147; ENSRNOG00000052620.
 GeneID; 83427; -.
 KEGG; rno:83427; -.
 UCSC; RGD:69229; rat.
 CTD; 10399; -.
 RGD; 69229; Rack1.
 eggNOG; KOG0279; Eukaryota.
 GeneTree; ENSGT00940000154461; -.
 HOGENOM; CLU_000288_57_7_1; -.
 InParanoid; P63245; -.
 OMA; CKAMLWD; -.
 OrthoDB; 805365at2759; -.
 PhylomeDB; P63245; -.
 TreeFam; TF300600; -.
 Reactome; R-RNO-5357905; Regulation of TNFR1 signaling.
 Reactome; R-RNO-5357956; TNFR1-induced NFkappaB signaling pathway.
 Reactome; R-RNO-5626978; TNFR1-mediated ceramide production.
 PRO; PR:P63245; -.
 Proteomes; UP000002494; Chromosome 10.
 Proteomes; UP000002494; Chromosome 12.
 Bgee; ENSRNOG00000049070; Expressed in pancreas and 17 other tissues.
 ExpressionAtlas; P63245; baseline and differential.
 Genevisible; P63245; RN.
 GO; GO:0044297; C:cell body; ISO:RGD.
 GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO; GO:0005829; C:cytosol; IBA:GO_Central.
 GO; GO:0030425; C:dendrite; ISO:RGD.
 GO; GO:1990630; C:IRE1-RACK1-PP2A complex; ISO:RGD.
 GO; GO:0016020; C:membrane; HDA:UniProtKB.
 GO; GO:0030496; C:midbody; ISS:UniProtKB.
 GO; GO:0005739; C:mitochondrion; ISO:RGD.
 GO; GO:0043005; C:neuron projection; ISO:RGD.
 GO; GO:0043025; C:neuronal cell body; ISS:UniProtKB.
 GO; GO:0005634; C:nucleus; ISS:UniProtKB.
 GO; GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell.
 GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
 GO; GO:0001891; C:phagocytic cup; ISS:UniProtKB.
 GO; GO:0015935; C:small ribosomal subunit; IDA:UniProtKB.
 GO; GO:0051434; F:BH3 domain binding; ISO:RGD.
 GO; GO:0030332; F:cyclin binding; ISO:RGD.
 GO; GO:0008656; F:cysteine-type endopeptidase activator activity involved in apoptotic process; ISO:RGD.
 GO; GO:0019899; F:enzyme binding; ISO:RGD.
 GO; GO:0008200; F:ion channel inhibitor activity; ISO:RGD.
 GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
 GO; GO:0005080; F:protein kinase C binding; IDA:UniProtKB.
 GO; GO:0019903; F:protein phosphatase binding; ISO:RGD.
 GO; GO:0030292; F:protein tyrosine kinase inhibitor activity; ISS:UniProtKB.
 GO; GO:0030971; F:receptor tyrosine kinase binding; ISS:UniProtKB.
 GO; GO:0043022; F:ribosome binding; ISS:UniProtKB.
 GO; GO:0042169; F:SH2 domain binding; ISS:UniProtKB.
 GO; GO:0035591; F:signaling adaptor activity; ISO:RGD.
 GO; GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; ISO:RGD.
 GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO; GO:0071333; P:cellular response to glucose stimulus; ISO:RGD.
 GO; GO:0071363; P:cellular response to growth factor stimulus; ISS:UniProtKB.
 GO; GO:0007369; P:gastrulation; IEA:UniProtKB-KW.
 GO; GO:0030308; P:negative regulation of cell growth; ISS:UniProtKB.
 GO; GO:0010629; P:negative regulation of gene expression; ISO:RGD.
 GO; GO:1903208; P:negative regulation of hydrogen peroxide-induced neuron death; ISO:RGD.
 GO; GO:0033137; P:negative regulation of peptidyl-serine phosphorylation; ISO:RGD.
 GO; GO:0050765; P:negative regulation of phagocytosis; ISS:UniProtKB.
 GO; GO:0032091; P:negative regulation of protein binding; ISO:RGD.
 GO; GO:0051898; P:negative regulation of protein kinase B signaling; ISO:RGD.
 GO; GO:0045879; P:negative regulation of smoothened signaling pathway; ISO:RGD.
 GO; GO:0017148; P:negative regulation of translation; IMP:UniProtKB.
 GO; GO:0030178; P:negative regulation of Wnt signaling pathway; ISS:UniProtKB.
 GO; GO:0001649; P:osteoblast differentiation; HDA:UniProtKB.
 GO; GO:0043473; P:pigmentation; ISO:RGD.
 GO; GO:0043065; P:positive regulation of apoptotic process; ISS:UniProtKB.
 GO; GO:0030335; P:positive regulation of cell migration; ISS:UniProtKB.
 GO; GO:0051343; P:positive regulation of cyclic-nucleotide phosphodiesterase activity; ISO:RGD.
 GO; GO:2000543; P:positive regulation of gastrulation; ISS:UniProtKB.
 GO; GO:0042998; P:positive regulation of Golgi to plasma membrane protein transport; ISS:UniProtKB.
 GO; GO:0043547; P:positive regulation of GTPase activity; ISS:UniProtKB.
 GO; GO:2001244; P:positive regulation of intrinsic apoptotic signaling pathway; ISO:RGD.
 GO; GO:0051901; P:positive regulation of mitochondrial depolarization; ISO:RGD.
 GO; GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO; GO:0001934; P:positive regulation of protein phosphorylation; ISS:UniProtKB.
 GO; GO:0031334; P:positive regulation of protein-containing complex assembly; ISS:UniProtKB.
 GO; GO:0007205; P:protein kinase C-activating G protein-coupled receptor signaling pathway; TAS:UniProtKB.
 GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
 GO; GO:0051726; P:regulation of cell cycle; ISS:UniProtKB.
 GO; GO:0051302; P:regulation of cell division; ISS:UniProtKB.
 GO; GO:2000114; P:regulation of establishment of cell polarity; ISS:UniProtKB.
 GO; GO:0032880; P:regulation of protein localization; ISS:UniProtKB.
 GO; GO:1903076; P:regulation of protein localization to plasma membrane; IMP:RGD.
 GO; GO:0072344; P:rescue of stalled ribosome; ISS:UniProtKB.
 GO; GO:0048511; P:rhythmic process; IEA:UniProtKB-KW.
 GO; GO:0006412; P:translation; ISO:RGD.
 Gene3D; 2.130.10.10; -; 1.
 InterPro; IPR045223; Asc1/RACK1.
 InterPro; IPR020472; G-protein_beta_WD-40_rep.
 InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
 InterPro; IPR001680; WD40_repeat.
 InterPro; IPR019775; WD40_repeat_CS.
 InterPro; IPR036322; WD40_repeat_dom_sf.
 PANTHER; PTHR19868; PTHR19868; 1.
 Pfam; PF00400; WD40; 7.
 PRINTS; PR00320; GPROTEINBRPT.
 SMART; SM00320; WD40; 7.
 SUPFAM; SSF50978; SSF50978; 1.
 PROSITE; PS00678; WD_REPEATS_1; 4.
 PROSITE; PS50082; WD_REPEATS_2; 6.
 PROSITE; PS50294; WD_REPEATS_REGION; 1.
 1: Evidence at protein level;
 Acetylation; Apoptosis; Biological rhythms; Cell cycle; Cell membrane;
 Cell projection; Cytoplasm; Developmental protein;
 Direct protein sequencing; Gastrulation; Growth regulation; Membrane;
 Nucleus; Phosphoprotein; Reference proteome; Repeat; Ribonucleoprotein;
 Ribosomal protein; Translation regulation; WD repeat.
 CHAIN           1..317
                 /note="Receptor of activated protein C kinase 1"
                 /id="PRO_0000127734"
 INIT_MET        1
                 /note="Removed; alternate"
                 /evidence="ECO:0000250|UniProtKB:P63244"
 CHAIN           2..317
                 /note="Receptor of activated protein C kinase 1, N-
                 terminally processed"
                 /id="PRO_0000424484"
 REPEAT          13..44
                 /note="WD 1"
 REPEAT          61..91
                 /note="WD 2"
 REPEAT          103..133
                 /note="WD 3"
 REPEAT          146..178
                 /note="WD 4"
 REPEAT          190..220
                 /note="WD 5"
 REPEAT          231..260
                 /note="WD 6"
 REPEAT          281..311
                 /note="WD 7"
 MOD_RES         1
                 /note="N-acetylmethionine"
                 /evidence="ECO:0000250|UniProtKB:P63244"
 MOD_RES         2
                 /note="N-acetylthreonine; in Guanine nucleotide-binding
                 protein subunit beta-2-like 1, N-terminally processed"
                 /evidence="ECO:0000250|UniProtKB:P63244"
 MOD_RES         6
                 /note="Phosphothreonine"
                 /evidence="ECO:0000250|UniProtKB:P63244"
 MOD_RES         10
                 /note="Phosphothreonine"
                 /evidence="ECO:0000250|UniProtKB:P63244"
 MOD_RES         52
                 /note="Phosphotyrosine; by ABL1"
                 /evidence="ECO:0000250|UniProtKB:P63244"
 MOD_RES         96
                 /note="Phosphothreonine"
                 /evidence="ECO:0000250|UniProtKB:P63244"
 MOD_RES         130
                 /note="N6-acetyllysine"
                 /evidence="ECO:0000250|UniProtKB:P63244"
 MOD_RES         183
                 /note="N6-acetyllysine"
                 /evidence="ECO:0000250|UniProtKB:P68040"
 MOD_RES         228
                 /note="Phosphotyrosine"
                 /evidence="ECO:0000250|UniProtKB:P63244"
 MOD_RES         276
                 /note="Phosphoserine"
                 /evidence="ECO:0000250|UniProtKB:P63244"
 MOD_RES         277
                 /note="Phosphothreonine"
                 /evidence="ECO:0000250|UniProtKB:P63244"
 MOD_RES         278
                 /note="Phosphoserine"
                 /evidence="ECO:0000250|UniProtKB:P63244"
 MOD_RES         279
                 /note="Phosphoserine"
                 /evidence="ECO:0000250|UniProtKB:P63244"
 MOD_RES         316
                 /note="Phosphothreonine"
                 /evidence="ECO:0000250|UniProtKB:P68040"
 CONFLICT        266
                 /note="I -> M (in Ref. 1; AAA18951)"
                 /evidence="ECO:0000305"
 SEQUENCE   317 AA;  35077 MW;  257F91E369ED2044 CRC64;
 MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR
 GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI
 VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA
 NCKLKTNHIG HTGYLNTVTV SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC
 FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA
 GYTDNLVRVW QVTIGTR

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