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Thrombin-like enzyme BpirSP27 (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)

 VSP27_BOTPI             Reviewed;          50 AA.
P0DL26;
01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
01-MAY-2013, sequence version 1.
12-AUG-2020, entry version 23.
RecName: Full=Thrombin-like enzyme BpirSP27;
Short=SVTLE;
EC=3.4.21.-;
AltName: Full=Fibrinogen-clotting enzyme;
AltName: Full=Snake venom serine protease;
Short=SVSP;
Flags: Fragment;
Bothrops pirajai (Piraja's lancehead).
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
Serpentes; Colubroidea; Viperidae; Crotalinae; Bothrops.
NCBI_TaxID=113192;
[1]
PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ACTIVITY
REGULATION, AND MASS SPECTROMETRY.
TISSUE=Venom;
PubMed=22819993; DOI=10.1016/j.biochi.2012.07.007;
Menaldo D.L., Bernardes C.P., Santos-Filho N.A., Moura Lde A., Fuly A.L.,
Arantes E.C., Sampaio S.V.;
"Biochemical characterization and comparative analysis of two distinct
serine proteases from Bothrops pirajai snake venom.";
Biochimie 94:2545-2558(2012).
[2]
FUNCTION, AND BIOASSAY.
PubMed=23499645; DOI=10.1016/j.intimp.2013.02.023;
Menaldo D.L., Bernardes C.P., Pereira J.C., Silveira D.S., Mamede C.C.,
Stanziola L., de Oliveira F., Pereira-Crott L.S., Faccioli L.H.,
Sampaio S.V.;
"Effects of two serine proteases from Bothrops pirajai snake venom on the
complement system and the inflammatory response.";
Int. Immunopharmacol. 15:764-771(2013).
-!- FUNCTION: Snake venom serine protease that interferes with the
hemostatic system of the prey. It preferentially degrades the Bbeta
chain (FGB) of fibrinogen, with minor effects on the Aalpha chain
(FGA). It presents a lower ability to degrade fibrin clots than
BpirSP41. It hydrolyzes chromogenic substrates S-2238 (used for testing
thrombin activity), S-2222 (factor Xa), S-2266 (glandular kallikrein
and factor XIa), S-2302 (plasma kallikrein, factor XIa and XIIa), and
S-2251 (plasmin). It shows a decrease in the clotting time of human
plasma in the presence of increasing doses of the enzyme. Its minimum
coagulant dose (MCD) is 3.5 ug. It also promotes platelet aggregation
in a concentration-dependent manner in the presence or absence of
calcium. It also shows 20% inhibition of the hemolytic activity
promoted by the complement pathways and possess only a minor role in
the induction of edema and pain in rat. {ECO:0000269|PubMed:22819993,
ECO:0000269|PubMed:23499645}.
-!- ACTIVITY REGULATION: Inhibited by serine protease inhibitors PMSF,
benzamidine, leupeptin and aprotinin, as well as by copper (Cu2+) and
manganese (Mn2+) ions. Not inhibited by metalloprotease inhibitors
EDTA, EGTA and 1,10-phenanthroline, as well as by barium (Ba2+) and
calcium ion (Ca2+). {ECO:0000269|PubMed:22819993}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
pH dependence:
Optimum pH is 6.0-10.5. {ECO:0000269|PubMed:22819993};
Temperature dependence:
Optimum temperature is 4-60 degrees Celsius.
{ECO:0000269|PubMed:22819993};
-!- SUBUNIT: Monomer. {ECO:0000250}.
-!- SUBCELLULAR LOCATION: Secreted.
-!- TISSUE SPECIFICITY: Expressed by the venom gland.
-!- PTM: N-glycosylated. {ECO:0000269|PubMed:22819993}.
-!- MASS SPECTROMETRY: Mass=27121; Method=MALDI;
Evidence={ECO:0000269|PubMed:22819993};
-!- MISCELLANEOUS: Acidic enzyme (pI is 4.7).
{ECO:0000305|PubMed:22819993}.
-!- MISCELLANEOUS: Does not degrade the gamma chain of fibrinogen (FGG).
{ECO:0000305|PubMed:22819993}.
-!- SIMILARITY: Belongs to the peptidase S1 family. Snake venom subfamily.
{ECO:0000255|PROSITE-ProRule:PRU00274}.
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SMR; P0DL26; -.
GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
Gene3D; 2.40.10.10; -; 1.
InterPro; IPR009003; Peptidase_S1_PA.
InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
InterPro; IPR001254; Trypsin_dom.
InterPro; IPR018114; TRYPSIN_HIS.
Pfam; PF00089; Trypsin; 1.
SUPFAM; SSF50494; SSF50494; 1.
PROSITE; PS00134; TRYPSIN_HIS; 1.
1: Evidence at protein level;
Blood coagulation cascade activating toxin;
Complement system impairing toxin; Direct protein sequencing;
Disulfide bond; Fibrinolytic toxin; Glycoprotein;
Hemostasis impairing toxin; Hydrolase;
Platelet aggregation activating toxin; Protease; Secreted; Serine protease;
Toxin.
CHAIN 1..>50
/note="Thrombin-like enzyme BpirSP27"
/id="PRO_0000422276"
DOMAIN 1..>50
/note="Peptidase S1"
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
ACT_SITE 41
/note="Charge relay system"
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274,
ECO:0000255|PROSITE-ProRule:PRU10078, ECO:0000255|PROSITE-
ProRule:PRU10079"
CARBOHYD 20
/note="N-linked (GlcNAc...) asparagine"
/evidence="ECO:0000255"
DISULFID 7..?
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
DISULFID 26..42
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
NON_TER 50
SEQUENCE 50 AA; 5534 MW; 6593C96FF11E0C03 CRC64;
VVGGDECNIN EHRSLVAIFN STGFFCSGIL LNQEWVLTAS HCDSTNFQMK


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Related Genes :
[] Thrombin-like enzyme BpirSP27 (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme BpirSP41 (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme BJ-48 (SVTLE BJ-48) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme cerastocytin (SVTLE) (EC 3.4.21.-) (C.cerastes platelet proaggregant protein) (CC-PPP) (Factor VIII activator) (Fibrinogen-clotting enzyme) (Proaggregant serine proteinase) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme stejnobin (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme bhalternin (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme LMR-47 (SVTLE LM-47) (SVTLE LMR-47) (EC 3.4.21.74) (Fibrinogen-clotting enzyme) (Gyroxin analog) (Snake venom serine protease) (SVSP) (Venombin A) (Fragment)
[] Thrombin-like enzyme ancrod (SVTLE) (EC 3.4.21.74) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Venombin A)
[] Thrombin-like enzyme elegaxobin-1 (SVTLE) (EC 3.4.21.-) (Elegaxobin I) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme BpSP-1 (SVTLE BpSP-1) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Thrombin-like enzyme BpSP-I) (SVTLE BpSP-I) (Fragment)
[] Thrombin-like enzyme Cdc SI (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme Cdc SII (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme gyroxin B1.4 (SVTLE gyroxin B1.4) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme gyroxin B1.3 (SVTLE gyroxin B1.3) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme gyroxin B1.7 (SVTLE gyroxin B1.7) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Factor V activator (FVA) (VLFVA) (EC 3.4.21.95) (Lebetina viper venom FV activator) (LVV-V) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme halystase (SVTLE) (EC 3.4.21.-) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme gyroxin B2.1 (SVTLE gyroxin B2.1) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Venom plasminogen activator LV-PA (EC 3.4.21.-) (LMUT0402S) (Plasminogen activating proteinase) (Snake venom serine protease) (SVSP)
[] Chymotrypsin-like protease VLCTLP (EC 3.4.21.-) (Snake venom serine protease) (SVSP) (Vipera Lebetina with chymotrypsin-like proteolytic activity)
[F2] Prothrombin (EC 3.4.21.5) (Coagulation factor II) [Cleaved into: Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain]
[] Venom plasminogen activator TSV-PA (EC 3.4.21.-) (Snake venom serine protease) (SVSP)
[] Snake venom serine protease 2 (SP2) (SVSP) (EC 3.4.21.-) (Jerdonobin-II)
[] Protein C activator (EC 3.4.21.-) (ACC-C) (Snake venom serine protease) (SVSP)
[] Coagulation factor X-activating enzyme heavy chain (EC 3.4.24.58) (Coagulation factor X-activating enzyme chain alpha) (Snake venom metalloproteinase) (SVMP) (VL factor X activator) (VLFXA heavy chain) [Cleaved into: Coagulation factor X-activating enzyme heavy chain alternate form]
[CORIN CRN TMPRSS10] Atrial natriuretic peptide-converting enzyme (EC 3.4.21.-) (Corin) (Heart-specific serine proteinase ATC2) (Pro-ANP-converting enzyme) (Transmembrane protease serine 10) [Cleaved into: Atrial natriuretic peptide-converting enzyme, N-terminal propeptide; Atrial natriuretic peptide-converting enzyme, activated protease fragment; Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment; Atrial natriuretic peptide-converting enzyme, 160 kDa soluble fragment; Atrial natriuretic peptide-converting enzyme, 100 kDa soluble fragment]
[] Venom prothrombin activator pseutarin-C catalytic subunit (PCCS) (vPA) (EC 3.4.21.6) (Venom coagulation factor Xa-like protease) [Cleaved into: Pseutarin-C catalytic subunit light chain; Pseutarin-C catalytic subunit heavy chain]
[] Snake venom serine protease LmrSP-4 (SVSP) (EC 3.4.21.-) (Fragment)
[] Alpha- and beta-fibrinogenase OhS1 (EC 3.4.21.-) (Snake venom serine protease) (SVSP)
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Bibliography :