GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, [email protected]

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, Gentaur another in time delivery

Thrombin-like enzyme BpirSP41 (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)

 VSP41_BOTPI             Reviewed;          50 AA.
P0DL27;
01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
01-MAY-2013, sequence version 1.
12-AUG-2020, entry version 22.
RecName: Full=Thrombin-like enzyme BpirSP41;
Short=SVTLE;
EC=3.4.21.-;
AltName: Full=Fibrinogen-clotting enzyme;
AltName: Full=Snake venom serine protease;
Short=SVSP;
Flags: Fragment;
Bothrops pirajai (Piraja's lancehead).
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
Serpentes; Colubroidea; Viperidae; Crotalinae; Bothrops.
NCBI_TaxID=113192;
[1]
PROTEIN SEQUENCE, FUNCTION, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL
PROPERTIES, AND MASS SPECTROMETRY.
TISSUE=Venom;
PubMed=22819993; DOI=10.1016/j.biochi.2012.07.007;
Menaldo D.L., Bernardes C.P., Santos-Filho N.A., Moura Lde A., Fuly A.L.,
Arantes E.C., Sampaio S.V.;
"Biochemical characterization and comparative analysis of two distinct
serine proteases from Bothrops pirajai snake venom.";
Biochimie 94:2545-2558(2012).
[2]
FUNCTION, AND BIOASSAY.
PubMed=23499645; DOI=10.1016/j.intimp.2013.02.023;
Menaldo D.L., Bernardes C.P., Pereira J.C., Silveira D.S., Mamede C.C.,
Stanziola L., de Oliveira F., Pereira-Crott L.S., Faccioli L.H.,
Sampaio S.V.;
"Effects of two serine proteases from Bothrops pirajai snake venom on the
complement system and the inflammatory response.";
Int. Immunopharmacol. 15:764-771(2013).
-!- FUNCTION: Snake venom serine protease that interferes with the
hemostatic system of the prey. It almost completely degrades both
Aalpha (FGA) and Bbeta (FGB) chains of fibrinogen. It presents a higher
ability to degrade fibrin clots than BpirSP27. It hydrolyzes
chromogenic substrates S-2238 (used for testing thrombin activity), S-
2222 (factor Xa), S-2266 (glandular kallikrein and factor XIa), and S-
2302 (plasma kallikrein, factor XIa and XIIa). It shows a decrease in
the clotting time of human plasma in the presence of increasing doses
of the enzyme. Its minimum coagulant dose (MCD) is 20 ug. It promotes
platelet aggregation with a maximum of aggregation of 20%, regardless
of the concentration increase or the presence of calcium. It also shows
40% inhibition of the hemolytic activity promoted by the complement
pathways and possess only a minor role in the induction of edema and
pain in rat. {ECO:0000269|PubMed:22819993,
ECO:0000269|PubMed:23499645}.
-!- ACTIVITY REGULATION: Inhibited by serine protease inhibitors PMSF,
benzamidine, leupeptin and aprotinin, as well as by copper ions (Cu2+).
Not inhibited by metalloprotease inhibitors EDTA, EGTA and 1,10-
phenanthroline, as well as by barium (Ba2+) and calcium ion (Ca2+).
{ECO:0000269|PubMed:22819993}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
pH dependence:
Optimum pH is 6.0-10.5. {ECO:0000269|PubMed:22819993};
Temperature dependence:
Optimum temperature is 4-60 degrees Celsius.
{ECO:0000269|PubMed:22819993};
-!- SUBUNIT: Monomer. {ECO:0000250}.
-!- SUBCELLULAR LOCATION: Secreted.
-!- TISSUE SPECIFICITY: Expressed by the venom gland.
-!- PTM: N-glycosylated. {ECO:0000269|PubMed:22819993}.
-!- MASS SPECTROMETRY: Mass=40639; Method=MALDI;
Evidence={ECO:0000269|PubMed:22819993};
-!- MISCELLANEOUS: Acidic enzyme (pI is 3.7).
{ECO:0000305|PubMed:22819993}.
-!- MISCELLANEOUS: Does not degrade the gamma chain of fibrinogen (FGG). It
has no activity on S-2251 (used for testing plasmin activity)
(PubMed:22819993). {ECO:0000305|PubMed:22819993}.
-!- SIMILARITY: Belongs to the peptidase S1 family. Snake venom subfamily.
{ECO:0000255|PROSITE-ProRule:PRU00274}.
---------------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
---------------------------------------------------------------------------
SMR; P0DL27; -.
GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
Gene3D; 2.40.10.10; -; 1.
InterPro; IPR009003; Peptidase_S1_PA.
InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
InterPro; IPR001254; Trypsin_dom.
InterPro; IPR018114; TRYPSIN_HIS.
Pfam; PF00089; Trypsin; 1.
SUPFAM; SSF50494; SSF50494; 1.
PROSITE; PS00134; TRYPSIN_HIS; 1.
1: Evidence at protein level;
Blood coagulation cascade activating toxin;
Complement system impairing toxin; Direct protein sequencing;
Disulfide bond; Fibrinolytic toxin; Glycoprotein;
Hemostasis impairing toxin; Hydrolase;
Platelet aggregation activating toxin; Protease; Secreted; Serine protease;
Toxin.
CHAIN 1..>50
/note="Thrombin-like enzyme BpirSP41"
/id="PRO_0000422277"
DOMAIN 1..>50
/note="Peptidase S1"
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
ACT_SITE 40
/note="Charge relay system"
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274,
ECO:0000255|PROSITE-ProRule:PRU10078, ECO:0000255|PROSITE-
ProRule:PRU10079"
DISULFID 7..?
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
DISULFID 25..41
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
NON_TER 50
SEQUENCE 50 AA; 5836 MW; 11457A5DF16B1303 CRC64;
VVGGDECDIN EHPFLAFLYS HGYFCGLTLI NQEWVLTAAH CDRRFMRIYL


Related products :

Catalog number Product name Quantity
18-661-15015 ADAM 17 - EC 3.4.24.86; A disintegrin and metalloproteinase domain 17; TNF-alpha-converting enzyme; TNF-alpha convertase; Snake venom-like protease; CD156b antigen Polyclonal 0.1 mg
U1319h CLIA ADAM 17,ADAM17,CSVP,Disintegrin and metalloproteinase domain-containing protein 17,Homo sapiens,Human,Snake venom-like protease,TACE,TNF-alpha convertase,TNF-alpha-converting enzyme 96T
E1319h ELISA ADAM 17,ADAM17,CSVP,Disintegrin and metalloproteinase domain-containing protein 17,Homo sapiens,Human,Snake venom-like protease,TACE,TNF-alpha convertase,TNF-alpha-converting enzyme 96T
E1319h ELISA kit ADAM 17,ADAM17,CSVP,Disintegrin and metalloproteinase domain-containing protein 17,Homo sapiens,Human,Snake venom-like protease,TACE,TNF-alpha convertase,TNF-alpha-converting enzyme 96T
EIAAB08673 Atrial natriuretic peptide-converting enzyme,Corin,CORIN,CRN,Heart-specific serine proteinase ATC2,Homo sapiens,Human,Pro-ANP-converting enzyme,TMPRSS10,Transmembrane protease serine 10
18-272-195435 TACE - Rabbit polyclonal to TACE; EC 3.4.24.86; A disintegrin and metalloproteinase domain 17; TNF-alpha-converting enzyme; TNF-alpha convertase; Snake venom-like protease; CD156b antigen Polyclonal 0.05 mg
18-272-195436 TACE - Rabbit polyclonal to TACE; EC 3.4.24.86; A disintegrin and metalloproteinase domain 17; TNF-alpha-converting enzyme; TNF-alpha convertase; Snake venom-like protease; CD156b antigen Polyclonal 0.05 mg
18-272-195437 TACE - Rabbit polyclonal to TACE; EC 3.4.24.86; A disintegrin and metalloproteinase domain 17; TNF-alpha-converting enzyme; TNF-alpha convertase; Snake venom-like protease; CD156b antigen Polyclonal 0.05 mg
18-272-197022 TACE - Rabbit polyclonal to TACE; EC 3.4.24.86; A disintegrin and metalloproteinase domain 17; TNF-alpha-converting enzyme; TNF-alpha convertase; Snake venom-like protease; CD156b antigen Polyclonal 0.1 mg
Y050525 Anti-cSVP(snake venom-like protease) Antibody 100ug
gen16977 ADA17_HUMAN Snake venom-like protease ELISA tesk kit 1
U0718m CLIA Asp 2,ASP2,Aspartyl protease 2,Bace,Bace1,Beta-secretase 1,Beta-site amyloid precursor protein cleaving enzyme 1,Beta-site APP cleaving enzyme 1,Memapsin-2,Membrane-associated aspartic protease 2 96T
U1812m CLIA Asp 1,ASP1,Aspartyl protease 1,Bace2,Beta-secretase 2,Beta-site amyloid precursor protein cleaving enzyme 2,Beta-site APP cleaving enzyme 2,Memapsin-1,Membrane-associated aspartic protease 1,Mous 96T
U0718r CLIA Asp 2,ASP2,Aspartyl protease 2,Bace,Bace1,Beta-secretase 1,Beta-site amyloid precursor protein cleaving enzyme 1,Beta-site APP cleaving enzyme 1,Memapsin-2,Membrane-associated aspartic protease 2 96T
E0718r ELISA Asp 2,ASP2,Aspartyl protease 2,Bace,Bace1,Beta-secretase 1,Beta-site amyloid precursor protein cleaving enzyme 1,Beta-site APP cleaving enzyme 1,Memapsin-2,Membrane-associated aspartic protease 96T
E0718m ELISA Asp 2,ASP2,Aspartyl protease 2,Bace,Bace1,Beta-secretase 1,Beta-site amyloid precursor protein cleaving enzyme 1,Beta-site APP cleaving enzyme 1,Memapsin-2,Membrane-associated aspartic protease 96T
E1812h ELISA kit AEPLC,ALP56,Asp 1,ASP1,ASP21,Aspartic-like protease 56 kDa,Aspartyl protease 1,BACE2,Beta-secretase 2,Beta-site amyloid precursor protein cleaving enzyme 2,Beta-site APP cleaving enzyme 2,C 96T
U1812h CLIA kit AEPLC,ALP56,Asp 1,ASP1,ASP21,Aspartic-like protease 56 kDa,Aspartyl protease 1,BACE2,Beta-secretase 2,Beta-site amyloid precursor protein cleaving enzyme 2,Beta-site APP cleaving enzyme 2,CD 96T
E1812h ELISA AEPLC,ALP56,Asp 1,ASP1,ASP21,Aspartic-like protease 56 kDa,Aspartyl protease 1,BACE2,Beta-secretase 2,Beta-site amyloid precursor protein cleaving enzyme 2,Beta-site APP cleaving enzyme 2,CDA13, 96T
U1812h CLIA AEPLC,ALP56,Asp 1,ASP1,ASP21,Aspartic-like protease 56 kDa,Aspartyl protease 1,BACE2,Beta-secretase 2,Beta-site amyloid precursor protein cleaving enzyme 2,Beta-site APP cleaving enzyme 2,CDA13,D 96T
U1812m CLIA kit Asp 1,ASP1,Aspartyl protease 1,Bace2,Beta-secretase 2,Beta-site amyloid precursor protein cleaving enzyme 2,Beta-site APP cleaving enzyme 2,Memapsin-1,Membrane-associated aspartic protease 1 96T
E1812m ELISA Asp 1,ASP1,Aspartyl protease 1,Bace2,Beta-secretase 2,Beta-site amyloid precursor protein cleaving enzyme 2,Beta-site APP cleaving enzyme 2,Memapsin-1,Membrane-associated aspartic protease 1,Mou 96T
E1812m ELISA kit Asp 1,ASP1,Aspartyl protease 1,Bace2,Beta-secretase 2,Beta-site amyloid precursor protein cleaving enzyme 2,Beta-site APP cleaving enzyme 2,Memapsin-1,Membrane-associated aspartic protease 96T
U1910m CLIA Kallikrein-7,Klk7,Mouse,Mus musculus,Prss6,Scce,Serine protease 6,Stratum corneum chymotryptic enzyme,Thymopsin 96T
E1910m ELISA kit Kallikrein-7,Klk7,Mouse,Mus musculus,Prss6,Scce,Serine protease 6,Stratum corneum chymotryptic enzyme,Thymopsin 96T
Pathways :
WP626: Abscisic Acid Biosynthesis
WP2340: Thiamine (vitamin B1) biosynthesis and salvage
WP2349: vitamin B3 (niacin), NAD and NADP biosynthesis pathway
WP1493: Carbon assimilation C4 pathway
WP2199: Seed Development
WP2344: vitamin B6 (pyridoxine, pyridoxal, pyridoxamine) biosynthesis and salvage pathway
WP272: Blood Clotting Cascade
WP142: mRNA processing
WP2341: vitamin B1 (thiamin) biosynthesis and salvage pathway
WP1122: Blood Clotting Cascade
WP459: Serine Biosynthesis
WP1659: Glycine, serine and threonine metabolism
WP218: Serine and Glycine biosynthesis
WP390: Serine-isocitrate lyase pathway
WP470: Proteasome Degradation
WP1929: Thrombin signalling through proteinase activated receptors (PARs)
WP887: Blood Clotting Cascade
WP460: Blood Clotting Cascade
WP1818: Formation of Fibrin Clot (Clotting Cascade)
WP775: Blood Clotting Cascade
WP2185: Purine metabolism
WP255: Blood Clotting Cascade

Related Genes :
[] Thrombin-like enzyme BpirSP41 (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme BpirSP27 (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme BJ-48 (SVTLE BJ-48) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme cerastocytin (SVTLE) (EC 3.4.21.-) (C.cerastes platelet proaggregant protein) (CC-PPP) (Factor VIII activator) (Fibrinogen-clotting enzyme) (Proaggregant serine proteinase) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme stejnobin (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme bhalternin (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme LMR-47 (SVTLE LM-47) (SVTLE LMR-47) (EC 3.4.21.74) (Fibrinogen-clotting enzyme) (Gyroxin analog) (Snake venom serine protease) (SVSP) (Venombin A) (Fragment)
[] Thrombin-like enzyme ancrod (SVTLE) (EC 3.4.21.74) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Venombin A)
[] Thrombin-like enzyme elegaxobin-1 (SVTLE) (EC 3.4.21.-) (Elegaxobin I) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme BpSP-1 (SVTLE BpSP-1) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Thrombin-like enzyme BpSP-I) (SVTLE BpSP-I) (Fragment)
[] Thrombin-like enzyme Cdc SI (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme Cdc SII (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme gyroxin B1.4 (SVTLE gyroxin B1.4) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme gyroxin B1.3 (SVTLE gyroxin B1.3) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme gyroxin B1.7 (SVTLE gyroxin B1.7) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Factor V activator (FVA) (VLFVA) (EC 3.4.21.95) (Lebetina viper venom FV activator) (LVV-V) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme halystase (SVTLE) (EC 3.4.21.-) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme gyroxin B2.1 (SVTLE gyroxin B2.1) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Venom plasminogen activator LV-PA (EC 3.4.21.-) (LMUT0402S) (Plasminogen activating proteinase) (Snake venom serine protease) (SVSP)
[] Chymotrypsin-like protease VLCTLP (EC 3.4.21.-) (Snake venom serine protease) (SVSP) (Vipera Lebetina with chymotrypsin-like proteolytic activity)
[F2] Prothrombin (EC 3.4.21.5) (Coagulation factor II) [Cleaved into: Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain]
[] Venom plasminogen activator TSV-PA (EC 3.4.21.-) (Snake venom serine protease) (SVSP)
[] Snake venom serine protease 2 (SP2) (SVSP) (EC 3.4.21.-) (Jerdonobin-II)
[] Protein C activator (EC 3.4.21.-) (ACC-C) (Snake venom serine protease) (SVSP)
[] Coagulation factor X-activating enzyme heavy chain (EC 3.4.24.58) (Coagulation factor X-activating enzyme chain alpha) (Snake venom metalloproteinase) (SVMP) (VL factor X activator) (VLFXA heavy chain) [Cleaved into: Coagulation factor X-activating enzyme heavy chain alternate form]
[CORIN CRN TMPRSS10] Atrial natriuretic peptide-converting enzyme (EC 3.4.21.-) (Corin) (Heart-specific serine proteinase ATC2) (Pro-ANP-converting enzyme) (Transmembrane protease serine 10) [Cleaved into: Atrial natriuretic peptide-converting enzyme, N-terminal propeptide; Atrial natriuretic peptide-converting enzyme, activated protease fragment; Atrial natriuretic peptide-converting enzyme, 180 kDa soluble fragment; Atrial natriuretic peptide-converting enzyme, 160 kDa soluble fragment; Atrial natriuretic peptide-converting enzyme, 100 kDa soluble fragment]
[] Venom prothrombin activator pseutarin-C catalytic subunit (PCCS) (vPA) (EC 3.4.21.6) (Venom coagulation factor Xa-like protease) [Cleaved into: Pseutarin-C catalytic subunit light chain; Pseutarin-C catalytic subunit heavy chain]
[] Snake venom serine protease LmrSP-4 (SVSP) (EC 3.4.21.-) (Fragment)
[] Alpha- and beta-fibrinogenase OhS1 (EC 3.4.21.-) (Snake venom serine protease) (SVSP)
[ADAM17 CSVP TACE] Disintegrin and metalloproteinase domain-containing protein 17 (ADAM 17) (EC 3.4.24.86) (Snake venom-like protease) (TNF-alpha convertase) (TNF-alpha-converting enzyme) (CD antigen CD156b)

Bibliography :