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Thrombin-like enzyme cerastocytin (SVTLE) (EC 3.4.21.-) (C.cerastes platelet proaggregant protein) (CC-PPP) (Factor VIII activator) (Fibrinogen-clotting enzyme) (Proaggregant serine proteinase) (Snake venom serine protease) (SVSP)

 VSPP_CERCE              Reviewed;         256 AA.
Q7SYF1; Q9PRT4;
10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
01-OCT-2003, sequence version 1.
02-DEC-2020, entry version 79.
RecName: Full=Thrombin-like enzyme cerastocytin;
Short=SVTLE;
EC=3.4.21.-;
AltName: Full=C.cerastes platelet proaggregant protein;
Short=CC-PPP;
AltName: Full=Factor VIII activator;
AltName: Full=Fibrinogen-clotting enzyme;
AltName: Full=Proaggregant serine proteinase;
AltName: Full=Snake venom serine protease;
Short=SVSP;
Flags: Precursor;
Cerastes cerastes (Horned desert viper).
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
Serpentes; Colubroidea; Viperidae; Viperinae; Cerastes.
NCBI_TaxID=8697;
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
TISSUE=Venom gland;
PubMed=12962484; DOI=10.1021/bi034790b;
Dekhil H., Wisner A., Marrakchi N., El Ayeb M., Bon C., Karoui H.;
"Molecular cloning and expression of a functional snake venom serine
proteinase, with platelet aggregating activity, from the Cerastes cerastes
viper.";
Biochemistry 42:10609-10618(2003).
[2]
PROTEIN SEQUENCE OF 25-74, FUNCTION, ACTIVITY REGULATION, AND SUBUNIT.
TISSUE=Venom;
PubMed=7766651; DOI=10.1016/0304-4165(94)00216-k;
Marrakchi N., Zingali R.B., Karoui H., Bon C., el Ayeb M.;
"Cerastocytin, a new thrombin-like platelet activator from the venom of the
Tunisian viper Cerastes cerastes.";
Biochim. Biophys. Acta 1244:147-156(1995).
[3]
FUNCTION.
PubMed=9080583; DOI=10.1016/s0041-0101(96)00116-x;
Marrakchi N., Barbouche R., Guermazi S., Bon C., el Ayeb M.;
"Procoagulant and platelet-aggregating properties of cerastocytin from
Cerastes cerastes venom.";
Toxicon 35:261-272(1997).
-!- FUNCTION: Thrombin-like snake venom serine protease which potently
induces platelet aggregation and has fibrinogenolytic activities. Clots
purified fibrinogen and hydrolyzes alpha-chains (FGA). High
concentrations of this enzyme also cleave prothrombin (F2) and factor X
(F10). Is also able to activate factor XIII (F8).
{ECO:0000269|PubMed:12962484, ECO:0000269|PubMed:7766651,
ECO:0000269|PubMed:9080583}.
-!- ACTIVITY REGULATION: Its platelets aggregating activity is inhibited by
chlorpromazine, theophylline mepacrine. Its platelet aggregating
activity and its amidolytic activity are inhibited by PMSF, TPCK, TLCK
and soybean trypsin inhibitors. Is unaffected by hirudin or by
antithrombin-III in the presence of heparin.
{ECO:0000269|PubMed:7766651}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=309 uM for S-2238 {ECO:0000269|PubMed:12962484};
KM=850 uM for S-2251 {ECO:0000269|PubMed:12962484};
-!- SUBUNIT: Monomer. {ECO:0000269|PubMed:7766651}.
-!- SUBCELLULAR LOCATION: Secreted.
-!- TISSUE SPECIFICITY: Expressed by the venom gland.
-!- SIMILARITY: Belongs to the peptidase S1 family. Snake venom subfamily.
{ECO:0000255|PROSITE-ProRule:PRU00274}.
-!- CAUTION: Lacks the conserved Cys-50-Cys-66 disulfide bridge due to the
replacement of Cys-50 by a Gly. {ECO:0000305}.
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EMBL; AJ553977; CAD86932.1; -; mRNA.
PIR; S55674; S55674.
SMR; Q7SYF1; -.
MEROPS; S01.497; -.
SABIO-RK; Q7SYF1; -.
GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
CDD; cd00190; Tryp_SPc; 1.
Gene3D; 2.40.10.10; -; 2.
InterPro; IPR009003; Peptidase_S1_PA.
InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
InterPro; IPR001314; Peptidase_S1A.
InterPro; IPR001254; Trypsin_dom.
InterPro; IPR018114; TRYPSIN_HIS.
InterPro; IPR033116; TRYPSIN_SER.
Pfam; PF00089; Trypsin; 1.
PRINTS; PR00722; CHYMOTRYPSIN.
SMART; SM00020; Tryp_SPc; 1.
SUPFAM; SSF50494; SSF50494; 1.
PROSITE; PS50240; TRYPSIN_DOM; 1.
PROSITE; PS00134; TRYPSIN_HIS; 1.
PROSITE; PS00135; TRYPSIN_SER; 1.
1: Evidence at protein level;
Blood coagulation cascade activating toxin; Direct protein sequencing;
Disulfide bond; Glycoprotein; Hemostasis impairing toxin; Hydrolase;
Platelet aggregation activating toxin; Protease; Secreted; Serine protease;
Signal; Toxin; Zymogen.
SIGNAL 1..18
/evidence="ECO:0000255"
PROPEP 19..24
/evidence="ECO:0000269|PubMed:7766651"
/id="PRO_0000294994"
CHAIN 25..256
/note="Thrombin-like enzyme cerastocytin"
/id="PRO_0000294995"
DOMAIN 25..247
/note="Peptidase S1"
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
ACT_SITE 65
/note="Charge relay system"
/evidence="ECO:0000250"
ACT_SITE 108
/note="Charge relay system"
/evidence="ECO:0000250"
ACT_SITE 202
/note="Charge relay system"
/evidence="ECO:0000250"
CARBOHYD 44
/note="N-linked (GlcNAc...) asparagine"
/evidence="ECO:0000255"
CARBOHYD 119
/note="N-linked (GlcNAc...) asparagine"
/evidence="ECO:0000255"
CARBOHYD 120
/note="N-linked (GlcNAc...) asparagine"
/evidence="ECO:0000255"
CARBOHYD 152
/note="N-linked (GlcNAc...) asparagine"
/evidence="ECO:0000255"
DISULFID 31..161
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
DISULFID 98..254
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
DISULFID 140..208
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
DISULFID 172..187
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
DISULFID 198..223
/evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
CONFLICT 44
/note="N -> T (in Ref. 2; AA sequence)"
/evidence="ECO:0000305"
SEQUENCE 256 AA; 27974 MW; 62F57976F89ECED1 CRC64;
MVLISVLASL LVLQLSYAQK SSELVIGGAE CNINEHRSLV LLYNSSRLFG GGTLINKEWV
LSAAHCDGEN MKIYLGLHHF RLPNKDRQIR VAKEKYFCRD RKSIVDKDIM LIKLNKPVNN
STHIAPLSLP SSPPSVGSDC RIMGWGTITS PNDTYPKVPH CANINILEHS LCERAYNDLS
ASSRTLCAGI EKGGIDTCKG DSGGPLICNG QIQGIVSWGD EVCGKPNKPG VYTKVFDYTD
WIRNIIAGNT AATCPQ


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Related Genes :
[] Thrombin-like enzyme cerastocytin (SVTLE) (EC 3.4.21.-) (C.cerastes platelet proaggregant protein) (CC-PPP) (Factor VIII activator) (Fibrinogen-clotting enzyme) (Proaggregant serine proteinase) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme BJ-48 (SVTLE BJ-48) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme stejnobin (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme BpirSP27 (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme BpirSP41 (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme bhalternin (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme LMR-47 (SVTLE LM-47) (SVTLE LMR-47) (EC 3.4.21.74) (Fibrinogen-clotting enzyme) (Gyroxin analog) (Snake venom serine protease) (SVSP) (Venombin A) (Fragment)
[] Factor V activator (FVA) (VLFVA) (EC 3.4.21.95) (Lebetina viper venom FV activator) (LVV-V) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme ancrod (SVTLE) (EC 3.4.21.74) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Venombin A)
[] Thrombin-like enzyme elegaxobin-1 (SVTLE) (EC 3.4.21.-) (Elegaxobin I) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Venom plasminogen activator LV-PA (EC 3.4.21.-) (LMUT0402S) (Plasminogen activating proteinase) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme BpSP-1 (SVTLE BpSP-1) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Thrombin-like enzyme BpSP-I) (SVTLE BpSP-I) (Fragment)
[] Thrombin-like enzyme Cdc SI (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme Cdc SII (SVTLE) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP) (Fragment)
[] Thrombin-like enzyme gyroxin B1.4 (SVTLE gyroxin B1.4) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme gyroxin B1.3 (SVTLE gyroxin B1.3) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme gyroxin B1.7 (SVTLE gyroxin B1.7) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme halystase (SVTLE) (EC 3.4.21.-) (Snake venom serine protease) (SVSP)
[] Thrombin-like enzyme gyroxin B2.1 (SVTLE gyroxin B2.1) (EC 3.4.21.-) (Fibrinogen-clotting enzyme) (Snake venom serine protease) (SVSP)
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[] Venom prothrombin activator pseutarin-C catalytic subunit (PCCS) (vPA) (EC 3.4.21.6) (Venom coagulation factor Xa-like protease) [Cleaved into: Pseutarin-C catalytic subunit light chain; Pseutarin-C catalytic subunit heavy chain]
[] Protein C activator (EC 3.4.21.-) (ACC-C) (Snake venom serine protease) (SVSP)
[rep 1a-1b] Replicase polyprotein 1ab (ORF1ab polyprotein) [Cleaved into: Nsp1 (EC 3.4.22.-); Nsp1-alpha papain-like cysteine proteinase (EC 3.4.22.-) (PCP1-alpha); Nsp1-beta papain-like cysteine proteinase (EC 3.4.22.-) (PCP1-beta); Nsp2 cysteine proteinase (EC 3.4.19.12) (EC 3.4.22.-) (CP2) (CP); Non-structural protein 3 (Nsp3); Serine protease nsp4 (3CLSP) (EC 3.4.21.-) (3C-like serine proteinase) (Nsp4); Non-structural protein 5-6-7 (Nsp5-6-7); Non-structural protein 5 (Nsp5); Non-structural protein 6 (Nsp6); Non-structural protein 7-alpha (Nsp7-alpha); Non-structural protein 7-beta (Nsp7-beta); Non-structural protein 8 (Nsp8); RNA-directed RNA polymerase (Pol) (RdRp) (EC 2.7.7.48) (Nsp9); Helicase nsp10 (Hel) (EC 3.6.4.12) (EC 3.6.4.13) (Nsp10); Uridylate-specific endoribonuclease nsp11 (EC 4.6.1.-) (Non-structural protein 11) (Nsp11); Non-structural protein 12 (Nsp12)]
[F2] Prothrombin (EC 3.4.21.5) (Coagulation factor II) [Cleaved into: Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain]
[rep 1a-1b] Replicase polyprotein 1ab (ORF1ab polyprotein) [Cleaved into: Nsp1 (EC 3.4.22.-); Nsp1-alpha papain-like cysteine proteinase (EC 3.4.22.-) (PCP1-alpha); Nsp1-beta papain-like cysteine proteinase (EC 3.4.22.-) (PCP1-beta); Nsp2 cysteine proteinase (EC 3.4.19.12) (EC 3.4.22.-) (CP2) (CP); Non-structural protein 3 (Nsp3); Serine protease nsp4 (3CLSP) (EC 3.4.21.-) (3C-like serine proteinase) (Nsp4); Non-structural protein 5-6-7 (Nsp5-6-7); Non-structural protein 5 (Nsp5); Non-structural protein 6 (Nsp6); Non-structural protein 7-alpha (Nsp7-alpha); Non-structural protein 7-beta (Nsp7-beta); Non-structural protein 8 (Nsp8); RNA-directed RNA polymerase (Pol) (RdRp) (EC 2.7.7.48) (Nsp9); Helicase nsp10 (Hel) (EC 3.6.4.12) (EC 3.6.4.13) (Nsp10); Uridylate-specific endoribonuclease nsp11 (EC 4.6.1.-) (Non-structural protein 11) (Nsp11); Non-structural protein 12 (Nsp12)]
[] Snake venom serine protease 2 (SP2) (SVSP) (EC 3.4.21.-) (Jerdonobin-II)
[ADAM17 CSVP TACE] Disintegrin and metalloproteinase domain-containing protein 17 (ADAM 17) (EC 3.4.24.86) (Snake venom-like protease) (TNF-alpha convertase) (TNF-alpha-converting enzyme) (CD antigen CD156b)
[] Snake venom serine protease IVa (SVSP) (EC 3.4.21.-) (Basic proteinase IVa) (Fragment)

Bibliography :