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Transcription factor E2F1 (E2F-1) (Fragment)

 E2F1_RAT                Reviewed;         432 AA.
O09139;
01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
01-APR-2015, sequence version 2.
13-FEB-2019, entry version 127.
RecName: Full=Transcription factor E2F1;
Short=E2F-1;
Flags: Fragment;
Name=E2f1;
Rattus norvegicus (Rat).
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
NCBI_TaxID=10116;
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=8673024;
Hosokawa Y., Yang M., Kaneko S., Tanaka M., Nakashima K.;
"Synergistic gene expressions of cyclin E, cdk2, cdk5 and E2F-1 during
the prolactin-induced G1/S transition in rat Nb2 pre-T lymphoma
cells.";
Biochem. Mol. Biol. Int. 37:393-399(1995).
[2]
INTERACTION WITH CEBPA.
PubMed=20176812; DOI=10.1128/MCB.01619-09;
Zaragoza K., Begay V., Schuetz A., Heinemann U., Leutz A.;
"Repression of transcriptional activity of C/EBPalpha by E2F-
dimerization partner complexes.";
Mol. Cell. Biol. 30:2293-2304(2010).
-!- FUNCTION: Transcription activator that binds DNA cooperatively
with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-
3' found in the promoter region of a number of genes whose
products are involved in cell cycle regulation or in DNA
replication. The DRTF1/E2F complex functions in the control of
cell-cycle progression from G1 to S phase. E2F1 binds
preferentially RB1 in a cell-cycle dependent manner. It can
mediate both cell proliferation and TP53/p53-dependent apoptosis.
Blocks adipocyte differentiation by binding to specific promoters
repressing CEBPA binding to its target gene promoters. Positively
regulates transcription of RRP1B. {ECO:0000250|UniProtKB:Q01094,
ECO:0000250|UniProtKB:Q61501}.
-!- ACTIVITY REGULATION: BIRC2/c-IAP1 stimulates its transcriptional
activity. {ECO:0000250}.
-!- SUBUNIT: Component of the DRTF1/E2F transcription factor complex.
Forms heterodimers with DP family members. The E2F1 complex binds
specifically hypophosphorylated retinoblastoma protein RB1. During
the cell cycle, RB1 becomes phosphorylated in mid-to-late G1
phase, detaches from the DRTF1/E2F complex, rendering E2F
transcriptionally active. Interacts with TRRAP, which probably
mediates its interaction with histone acetyltransferase complexes,
leading to transcription activation. Binds TOPBP1 and EAPP.
Interacts with ARID3A. Interacts with TRIM28; the interaction
inhibits E2F1 acetylation through recruiting HDAC1 and represses
its transcriptional activity. Interaction with KAT2B; the
interaction acetylates E2F1 enhancing its DNA-binding and
transcriptional activity. Interacts with BIRC2/c-IAP1 (via BIR
domains). The complex TFDP1:E2F1 interacts with CEBPA; the
interaction prevents CEBPA binding to target genes promoters and
represses its transcriptional activity. Interacts with RRP1B.
Interacts with HCFC1. Interacts with KMT2E; the interaction is
probably indirect and is mediated via HCFC1.
{ECO:0000250|UniProtKB:Q01094, ECO:0000250|UniProtKB:Q61501}.
-!- INTERACTION:
P33568:Rb1; NbExp=2; IntAct=EBI-1211101, EBI-1162932;
-!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q01094}.
-!- PTM: Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Phosphorylation by CHEK2 stabilizes E2F1 upon DNA damage and
regulates its effect on transcription and apoptosis (By
similarity). {ECO:0000250}.
-!- PTM: Acetylation stimulates DNA-binding. Enhanced under stress
conditions such as DNA damage and inhibited by retinoblastoma
protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1
resulting in deacetylation (By similarity). {ECO:0000250}.
-!- SIMILARITY: Belongs to the E2F/DP family. {ECO:0000305}.
-----------------------------------------------------------------------
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EMBL; D63165; BAA09641.1; -; mRNA.
RefSeq; NP_001094248.1; NM_001100778.1.
UniGene; Rn.72471; -.
SMR; O09139; -.
IntAct; O09139; 1.
STRING; 10116.ENSRNOP00000022428; -.
PaxDb; O09139; -.
PRIDE; O09139; -.
Ensembl; ENSRNOT00000022428; ENSRNOP00000022428; ENSRNOG00000016708.
GeneID; 399489; -.
KEGG; rno:399489; -.
UCSC; RGD:728892; rat.
CTD; 1869; -.
RGD; 728892; E2f1.
eggNOG; KOG2577; Eukaryota.
eggNOG; ENOG410XNYI; LUCA.
GeneTree; ENSGT00940000159472; -.
HOGENOM; HOG000232045; -.
InParanoid; O09139; -.
KO; K17454; -.
OMA; VRCQDLR; -.
OrthoDB; 1087250at2759; -.
Reactome; R-RNO-68689; CDC6 association with the ORC:origin complex.
Reactome; R-RNO-68911; G2 Phase.
Reactome; R-RNO-69231; Cyclin D associated events in G1.
Proteomes; UP000002494; Chromosome 3.
Bgee; ENSRNOG00000016708; Expressed in 9 organ(s), highest expression level in brain.
ExpressionAtlas; O09139; baseline and differential.
Genevisible; O09139; RN.
GO; GO:0005813; C:centrosome; IEA:Ensembl.
GO; GO:0005737; C:cytoplasm; IDA:RGD.
GO; GO:0000790; C:nuclear chromatin; IEA:Ensembl.
GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
GO; GO:0005634; C:nucleus; IDA:RGD.
GO; GO:0035189; C:Rb-E2F complex; IEA:Ensembl.
GO; GO:0090575; C:RNA polymerase II transcription factor complex; IBA:GO_Central.
GO; GO:0003677; F:DNA binding; IBA:GO_Central.
GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
GO; GO:0019901; F:protein kinase binding; IPI:RGD.
GO; GO:0000987; F:proximal promoter sequence-specific DNA binding; IEA:Ensembl.
GO; GO:0043565; F:sequence-specific DNA binding; IDA:RGD.
GO; GO:0008134; F:transcription factor binding; IBA:GO_Central.
GO; GO:0043276; P:anoikis; IEA:Ensembl.
GO; GO:0071398; P:cellular response to fatty acid; IEP:RGD.
GO; GO:0071456; P:cellular response to hypoxia; IEP:RGD.
GO; GO:1990090; P:cellular response to nerve growth factor stimulus; IDA:RGD.
GO; GO:0071466; P:cellular response to xenobiotic stimulus; IEA:Ensembl.
GO; GO:0000077; P:DNA damage checkpoint; ISS:UniProtKB.
GO; GO:0030900; P:forebrain development; IEA:Ensembl.
GO; GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:RGD.
GO; GO:0072332; P:intrinsic apoptotic signaling pathway by p53 class mediator; IEA:Ensembl.
GO; GO:0008630; P:intrinsic apoptotic signaling pathway in response to DNA damage; ISS:UniProtKB.
GO; GO:1990086; P:lens fiber cell apoptotic process; IEA:Ensembl.
GO; GO:0048255; P:mRNA stabilization; IEA:Ensembl.
GO; GO:0043392; P:negative regulation of DNA binding; ISS:UniProtKB.
GO; GO:0045599; P:negative regulation of fat cell differentiation; ISS:UniProtKB.
GO; GO:0070345; P:negative regulation of fat cell proliferation; ISS:UniProtKB.
GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
GO; GO:0071930; P:negative regulation of transcription involved in G1/S transition of mitotic cell cycle; IEA:Ensembl.
GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
GO; GO:0043065; P:positive regulation of apoptotic process; IEA:Ensembl.
GO; GO:0048146; P:positive regulation of fibroblast proliferation; IEA:Ensembl.
GO; GO:0060252; P:positive regulation of glial cell proliferation; IMP:RGD.
GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
GO; GO:0051726; P:regulation of cell cycle; IBA:GO_Central.
GO; GO:2000045; P:regulation of G1/S transition of mitotic cell cycle; IEA:Ensembl.
GO; GO:0006355; P:regulation of transcription, DNA-templated; ISS:UniProtKB.
GO; GO:0007283; P:spermatogenesis; IEP:RGD.
GO; GO:0006351; P:transcription, DNA-templated; ISS:UniProtKB.
CDD; cd14660; E2F_DD; 1.
Gene3D; 1.10.10.10; -; 1.
InterPro; IPR015633; E2F.
InterPro; IPR037241; E2F-DP_heterodim.
InterPro; IPR032198; E2F_CC-MB.
InterPro; IPR003316; E2F_WHTH_DNA-bd_dom.
InterPro; IPR036388; WH-like_DNA-bd_sf.
InterPro; IPR036390; WH_DNA-bd_sf.
PANTHER; PTHR12081; PTHR12081; 1.
Pfam; PF16421; E2F_CC-MB; 1.
Pfam; PF02319; E2F_TDP; 1.
SMART; SM01372; E2F_TDP; 1.
SUPFAM; SSF144074; SSF144074; 1.
SUPFAM; SSF46785; SSF46785; 1.
1: Evidence at protein level;
Acetylation; Activator; Complete proteome; DNA-binding; Nucleus;
Phosphoprotein; Reference proteome; Transcription;
Transcription regulation.
CHAIN 1 432 Transcription factor E2F1.
/FTId=PRO_0000219463.
DNA_BIND 108 192 {ECO:0000255}.
REGION 65 106 Cyclin A:CDK2 binding.
{ECO:0000250|UniProtKB:Q01094}.
REGION 87 189 Interaction with BIRC2/c-IAP1.
{ECO:0000250|UniProtKB:Q01094}.
REGION 151 172 Leucine-zipper.
{ECO:0000250|UniProtKB:Q01094}.
REGION 190 377 Required for interaction with TRIM28.
{ECO:0000250|UniProtKB:Q01094}.
REGION 193 282 Dimerization. {ECO:0000255}.
REGION 363 432 Transactivation.
{ECO:0000250|UniProtKB:Q01094}.
REGION 404 421 RB1 binding.
{ECO:0000250|UniProtKB:Q01094}.
MOTIF 156 192 DEF box. {ECO:0000250|UniProtKB:Q01094}.
MOD_RES 115 115 N6-acetyllysine.
{ECO:0000250|UniProtKB:Q01094}.
MOD_RES 118 118 N6-acetyllysine.
{ECO:0000250|UniProtKB:Q01094}.
MOD_RES 123 123 N6-acetyllysine.
{ECO:0000250|UniProtKB:Q01094}.
MOD_RES 370 370 Phosphoserine.
{ECO:0000250|UniProtKB:Q01094}.
SEQUENCE 432 AA; 46548 MW; 4E401ADF6E20C13C CRC64;
MAVAPAGGQH APALEALLGA GALRLLDSSQ IVIISTAPDV GAPQVPTGPA APPAGPRDPD
VLLFATPQAP RPAPSAPRPA LGRPPVKRRL DLETDHQYLA GSSGPFRGRG RHPGKGVKSP
GEKSRYETSL NLTTKRFLEL LSHSADGVVD LNWAAEVLKV QKRRIYDITN VLEGIQLIAK
KSKNHIQWLG SRTMVGIGQR LEGLTQDLQQ LQESEQQLDH LMHICTTQLQ LLSEDSDIQR
LAYVTCQDLR SIADPAEQMV IVIKAPPETQ LQAVDSAETF QISLKSKQGP IDVFLCPEES
AEGISPGRTS YQETSGEDRN ADSGTAGPPP SPPSTSPTLD PSQSLLGLEQ EAVLPRIGNL
RAPMEEDRLS PLVAADSLLE HVKEDFSGLL PGEFISLSPP HEAVDYHFGL EEGEGIRDLF
DCDFGDLTPL DF


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E2F1-112AP E2F1 Transcription factor 1 (mid-region) Antigenic peptide: P-E2F1-2 Host: Rabbit Affinity purifed 100ul
E2F1-3-121AP PC-E2F1 (WB control) E2F1 Transcription factor 1 (C-epitope) Immunogen: peptide (24mer) Host: Rabbit 100-150ul
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E2F1-112AP E2F1-2-Biotin (Biotin Conjugates) E2F1 Transcription factor 1 (mid-region) Immunogen: peptide (17mer) Host: Rabbit 200ul
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Kits Elisa; taq POLYMERASE

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Pathways :
WP1046: Signaling of Hepatocyte Growth Factor Receptor
WP1058: Senescence and Autophagy
WP1066: Eukaryotic Transcription Initiation
WP1078: G1 to S cell cycle control
WP1083: Cell cycle
WP1105: Adipogenesis
WP1162: Signaling of Hepatocyte Growth Factor Receptor
WP1174: Senescence and Autophagy
WP1182: Eukaryotic Transcription Initiation
WP1195: G1 to S cell cycle control
WP1200: Cell cycle
WP1206: Signaling of Hepatocyte Growth Factor Receptor
WP1235: Signaling of Hepatocyte Growth Factor Receptor
WP1238: Eukaryotic Transcription Initiation
WP1267: Senescence and Autophagy
WP1305: Senescence and Autophagy
WP1378: Senescence and Autophagy
WP1530: miRNA regulation of DNA Damage Response
WP1537: E2F/MIRHG1 feedback-loop
WP1541: Energy Metabolism
WP1545: miRNAs involved in DDR
WP155: Adipogenesis
WP1579: DNA damage response
WP177: Eukaryotic Transcription Initiation
WP1789: Binding of RNA by Insulin-like Growth Factor-2 mRNA Binding Proteins (IGF2BPs/IMPs/VICKZs)

Related Genes :
[E2F1 RBBP3] Transcription factor E2F1 (E2F-1) (PBR3) (Retinoblastoma-associated protein 1) (RBAP-1) (Retinoblastoma-binding protein 3) (RBBP-3) (pRB-binding protein E2F-1)
[E2f1] Transcription factor E2F1 (E2F-1) (Fragment)
[E2f1] Transcription factor E2F1 (E2F-1)
[E2FB E2F1 At5g22220 T6G21] Transcription factor E2FB (E2F transcription factor-1) (AtE2F1)
[E2f2 CG1071] Transcription factor E2F2 (dE2F2) (E2F transcription factor 2) (E2F-like transcription factor E2F2)
[E2F1] Transcription factor E2F1 (E2F-1)
[E2FA E2F3 E2F4 At2g36010 F11F19.8] Transcription factor E2FA (E2F transcription factor-3) (AtE2F3)
[E2F8] Transcription factor E2F8 (E2F-8)
[E2f8] Transcription factor E2F8 (E2F-8)
[E2F7] Transcription factor E2F7 (E2F-7)
[E2f7] Transcription factor E2F7 (E2F-7)
[E2FC E2F2 At1g47870 T2E6.2] Transcription factor E2FC (E2F transcription factor-2) (AtE2F2)
[TFDP1 DP1] Transcription factor Dp-1 (DRTF1-polypeptide 1) (DRTF1) (E2F dimerization partner 1)
[E2F4] Transcription factor E2F4 (E2F-4)
[Tfdp1] Transcription factor Dp-1 (DRTF1-polypeptide 1) (E2F dimerization partner 1)
[E2F5] Transcription factor E2F5 (E2F-5)
[TFDP2 DP2] Transcription factor Dp-2 (E2F dimerization partner 2)
[Tfdp2 Dp2] Transcription factor Dp-2 (Dp-3) (E2F dimerization partner 2)
[E2F2] Transcription factor E2F2 (E2F-2)
[E2FD DEL2 E2L1 ELP3 At5g14960 F2G14.80] E2F transcription factor-like E2FD (DP-E2F-like protein 2) (E2F-like repressor E2L1)
[E2f2] Transcription factor E2F2 (E2F-2)
[E2f5] Transcription factor E2F5 (E2F-5)
[E2f3] Transcription factor E2F3 (E2F-3)
[E2F3 KIAA0075] Transcription factor E2F3 (E2F-3)
[E2f4] Transcription factor E2F4 (E2F-4)
[SUZ12 CHET9 JJAZ1 KIAA0160] Polycomb protein SUZ12 (Chromatin precipitated E2F target 9 protein) (ChET 9 protein) (Joined to JAZF1 protein) (Suppressor of zeste 12 protein homolog)
[E2F7] Transcription factor E2F7 (E2F-7)
[E2FF DEL3 E2L2 ELP1 At3g01330 T22N4.4] E2F transcription factor-like E2FF (DP-E2F-like protein 3) (E2F-like repressor E2L2)
[E2f7] Transcription factor E2F7 (E2F-7)
[E2f8] Transcription factor E2F8 (E2F-8)

Bibliography :
[17927874] [Construction of a eukaryotic vector expressing E2F-1 Gene and its effect on proliferation of gastric cancer cell line MKN-45].
[15255934] Inhibition of the E2F-1/p53/Bax pathway by tauroursodeoxycholic acid in amyloid beta-peptide-induced apoptosis of PC12 cells.
[12598654] Crystal structure of the retinoblastoma tumor suppressor protein bound to E2F and the molecular basis of its regulation.
[10597240] Association of Pur alpha and E2F-1 suppresses transcriptional activity of E2F-1.
[9920380] Solution structure of a fragment of the dimerization domain of DP-1 determined by 1H-nuclear magnetic resonance and distance geometry.
[9774713] Solution structure of a fragment of the dimerization domain of E2F-1 determined by circular dichroism, 1H nuclear magnetic resonance and distance geometry.
[9361194] Characterization of interior cleavage of retinoblastoma protein in apoptosis.
[8289811] The adenovirus E4-6/7 protein transactivates the E2 promoter by inducing dimerization of a heteromeric E2F complex.
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