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tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)

 A0A0R2V2Z7_9PROT        Unreviewed;       331 AA.
A0A0R2V2Z7;
20-JAN-2016, integrated into UniProtKB/TrEMBL.
20-JAN-2016, sequence version 1.
16-JAN-2019, entry version 16.
RecName: Full=tRNA-dihydrouridine(20/20a) synthase {ECO:0000256|HAMAP-Rule:MF_02041};
EC=1.3.1.- {ECO:0000256|HAMAP-Rule:MF_02041};
EC=1.3.1.91 {ECO:0000256|HAMAP-Rule:MF_02041};
AltName: Full=U20-specific dihydrouridine synthase {ECO:0000256|HAMAP-Rule:MF_02041};
Short=U20-specific Dus {ECO:0000256|HAMAP-Rule:MF_02041};
AltName: Full=tRNA-dihydrouridine synthase A {ECO:0000256|HAMAP-Rule:MF_02041};
Name=dusA {ECO:0000256|HAMAP-Rule:MF_02041};
ORFNames=ABS16_02665 {ECO:0000313|EMBL:KRP06070.1};
Pelagibacteraceae bacterium BACL20 MAG-120920-bin64.
Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales;
Pelagibacteraceae.
NCBI_TaxID=1655618 {ECO:0000313|EMBL:KRP06070.1};
[1] {ECO:0000313|EMBL:KRP06070.1}
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=BACL1 MAG-120920-bin64 {ECO:0000313|EMBL:KRP06070.1};
Hugerth L.W., Larsson J., Alneberg J., Lindh M.V., Legrand C.,
Pinhassi J., Andersson A.;
"Metagenome-Assembled Genomes uncover a global brackish microbiome.";
Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
-!- FUNCTION: Catalyzes the synthesis of 5,6-dihydrouridine (D), a
modified base found in the D-loop of most tRNAs, via the reduction
of the C5-C6 double bond in target uridines. Specifically modifies
U20 and U20a in tRNAs. {ECO:0000256|HAMAP-Rule:MF_02041}.
-!- CATALYTIC ACTIVITY:
Reaction=5,6-dihydrouridine(20) in tRNA + NAD(+) = H(+) + NADH +
uridine(20) in tRNA; Xref=Rhea:RHEA:53340, Rhea:RHEA-COMP:13533,
Rhea:RHEA-COMP:13534, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
EC=1.3.1.91; Evidence={ECO:0000256|HAMAP-Rule:MF_02041};
-!- CATALYTIC ACTIVITY:
Reaction=5,6-dihydrouridine(20) in tRNA + NADP(+) = H(+) + NADPH +
uridine(20) in tRNA; Xref=Rhea:RHEA:53336, Rhea:RHEA-COMP:13533,
Rhea:RHEA-COMP:13534, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
EC=1.3.1.91; Evidence={ECO:0000256|HAMAP-Rule:MF_02041};
-!- CATALYTIC ACTIVITY:
Reaction=5,6-dihydrouridine(20a) in tRNA + NAD(+) = H(+) + NADH +
uridine(20a) in tRNA; Xref=Rhea:RHEA:53348, Rhea:RHEA-
COMP:13535, Rhea:RHEA-COMP:13536, ChEBI:CHEBI:15378,
ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65315,
ChEBI:CHEBI:74443; Evidence={ECO:0000256|HAMAP-Rule:MF_02041};
-!- CATALYTIC ACTIVITY:
Reaction=5,6-dihydrouridine(20a) in tRNA + NADP(+) = H(+) + NADPH
+ uridine(20a) in tRNA; Xref=Rhea:RHEA:53344, Rhea:RHEA-
COMP:13535, Rhea:RHEA-COMP:13536, ChEBI:CHEBI:15378,
ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:65315,
ChEBI:CHEBI:74443; Evidence={ECO:0000256|HAMAP-Rule:MF_02041};
-!- COFACTOR:
Name=FMN; Xref=ChEBI:CHEBI:58210;
Evidence={ECO:0000256|HAMAP-Rule:MF_02041,
ECO:0000256|PIRNR:PIRNR006621};
-!- SIMILARITY: Belongs to the Dus family. DusA subfamily.
{ECO:0000256|HAMAP-Rule:MF_02041}.
-!- SIMILARITY: Belongs to the dus family.
{ECO:0000256|PIRNR:PIRNR006621}.
-!- CAUTION: Lacks conserved residue(s) required for the propagation
of feature annotation. {ECO:0000256|HAMAP-Rule:MF_02041}.
-!- CAUTION: The sequence shown here is derived from an
EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
preliminary data. {ECO:0000313|EMBL:KRP06070.1}.
-----------------------------------------------------------------------
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Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; LICT01000001; KRP06070.1; -; Genomic_DNA.
GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
GO; GO:0010181; F:FMN binding; IEA:UniProtKB-UniRule.
GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
GO; GO:0102264; F:tRNA-dihydrouridine20 synthase activity; IEA:UniProtKB-EC.
CDD; cd02801; DUS_like_FMN; 1.
Gene3D; 3.20.20.70; -; 1.
HAMAP; MF_02041; DusA_subfam; 1.
InterPro; IPR013785; Aldolase_TIM.
InterPro; IPR035587; DUS-like_FMN-bd.
InterPro; IPR004653; DusA.
InterPro; IPR001269; tRNA_hU_synthase.
InterPro; IPR018517; tRNA_hU_synthase_CS.
PANTHER; PTHR42907; PTHR42907; 1.
Pfam; PF01207; Dus; 1.
PIRSF; PIRSF006621; Dus; 1.
TIGRFAMs; TIGR00742; yjbN; 1.
PROSITE; PS01136; UPF0034; 1.
3: Inferred from homology;
Flavoprotein {ECO:0000256|HAMAP-Rule:MF_02041,
ECO:0000256|PIRNR:PIRNR006621};
FMN {ECO:0000256|HAMAP-Rule:MF_02041, ECO:0000256|PIRNR:PIRNR006621};
NADP {ECO:0000256|HAMAP-Rule:MF_02041};
Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_02041,
ECO:0000256|PIRNR:PIRNR006621};
RNA-binding {ECO:0000256|HAMAP-Rule:MF_02041};
tRNA processing {ECO:0000256|HAMAP-Rule:MF_02041,
ECO:0000256|PIRNR:PIRNR006621};
tRNA-binding {ECO:0000256|HAMAP-Rule:MF_02041}.
NP_BIND 12 14 FMN. {ECO:0000256|HAMAP-Rule:MF_02041}.
NP_BIND 206 208 FMN. {ECO:0000256|HAMAP-Rule:MF_02041}.
NP_BIND 228 229 FMN. {ECO:0000256|HAMAP-Rule:MF_02041}.
ACT_SITE 95 95 Proton donor. {ECO:0000256|HAMAP-
Rule:MF_02041,
ECO:0000256|PIRSR:PIRSR006621-1}.
BINDING 65 65 FMN. {ECO:0000256|HAMAP-Rule:MF_02041}.
BINDING 134 134 FMN. {ECO:0000256|HAMAP-Rule:MF_02041}.
BINDING 166 166 FMN. {ECO:0000256|HAMAP-Rule:MF_02041}.
SITE 92 92 Interacts with tRNA. {ECO:0000256|HAMAP-
Rule:MF_02041}.
SITE 178 178 Interacts with tRNA; defines subfamily-
specific binding signature.
{ECO:0000256|HAMAP-Rule:MF_02041}.
SEQUENCE 331 AA; 37535 MW; 59ACFEF81ED222F5 CRC64;
MKNISRKISV APMMDCTDRH DRYFLRLMSK NVMLYSEMVA TKSAIHGDRK KILSYSPEEK
PLALQVGGSD KAELAEVAKI AEDMGYDEIN INLGCPSKKV QKNMFGACLM REPDLVAECI
NSMVNACKIP VTAKTRIGFD DTEEFDYLNN FILKMKGVGC STFILHARKA ILTGMSPKQN
LNIPKLNYGM VYQVKKENPD LEIIINGGIS KIDEIKNHLT LCDGVMIGRS IYQNPYSLVE
IEKEFFGATE HPTREQVAEK LLEYLEKEVK LGTKVNHIMR HTVGLYHGQV GSKDWKRYLS
DNMMARDSDF QKAKHIMTIV QNNEKAIQSN L


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Kits Elisa; taq POLYMERASE

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Pathways :
WP1403: AMPK signaling
WP1422: Sphingolipid Metabolism
WP1423: Ganglio Sphingolipid Metabolism
WP1424: Globo Sphingolipid Metabolism
WP1461: Photosynthetic Carbon Reduction
WP1621: Arginine and proline metabolism
WP1708: Terpenoid backbone biosynthesis
WP2199: Seed Development
WP2208: Cardiolipin Biosynthesis
WP2210: Oryzalexin S biosynthesis
WP2211: Geranylgeranyldiphosphate biosynthesis II (plastidic)
WP2248: anthocyanin biosynthesis
WP2340: Thiamine (vitamin B1) biosynthesis and salvage
WP2341: vitamin B1 (thiamin) biosynthesis and salvage pathway
WP296: TCA Cycle - biocyc
WP626: Abscisic Acid Biosynthesis
WP668: Octadecanoid Pathway
WP677: Momilactone biosynthesis
WP106: Alanine and aspartate metabolism
WP1620: Aminoacyl-tRNA biosynthesis
WP1938: tRNA Aminoacylation
WP219: Cytoplasmic tRNA Synthetases
WP433: tRNA Synthetases
WP62: Mitochondrial tRNA Synthetases

Related Genes :
[dusA yjbN b4049 JW5950] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[ispH A0O31_01017] Multifunctional fusion protein [Includes: tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (DusA-like U20-specific dihydrouridine synthase) (U20-specific Dus); 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HMBPP reductase) (EC 1.17.7.4)]
[dus TTHA0016] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (DusA-like U20-specific dihydrouridine synthase) (U20-specific Dus)
[dus_3 dus_1 dus_2 dusA NCTC11679_05355 NCTC1936_00419 NCTC5047_04245 NCTC5052_05236 NCTC5053_03679 NCTC9601_05166 NCTC9617_03367 NCTC9661_05787 PMK1_01910 SAMEA3649709_04620] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA SAMN05880582_101916] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[yjbN dusA A6581_03815 A9819_23140 AC789_1c44580 ACN77_13265 ACN81_05795 AW059_18320 B1K96_03640 B7C53_20030 B9M99_07285 B9T59_11200 BB545_04715 BE963_11655 BHS81_24320 BIU72_20630 BN17_40231 BTQ06_28285 BUE81_22495 BVL39_04970 BWP17_09085 BXT93_20165 BZL69_05000 C2U48_12360 C4J69_17345 C4M78_06330 C5N07_20225 C5P01_10975 C5P43_26750 C6669_07250 C7235_23350 C7B02_13830 CA593_05650 CG691_24515 CG705_24980 CG706_06955 CIJ94_13445 COD46_18670 CR538_23810 CRD98_24950 CRM83_18265 CVH05_02240 CWS33_16005 CXB56_26125 D0X26_12230 D3I61_23865 DD762_09595 DL545_23295 DNQ41_01750 DQE83_12890 DS732_01940 DTL84_03985 EC3234A_77c00420 EL75_4140 EL79_4345 EL80_4252 ERS085374_01630 ERS085383_01453 ERS085404_01248 HMPREF3040_01367 NCTC10090_02700 NCTC10418_07037 NCTC10764_02883 NCTC10865_05778 NCTC11112_02403 NCTC11181_01881 NCTC11341_02874 NCTC12950_05132 NCTC7152_04647 NCTC7927_05171 NCTC8450_00470 NCTC8985_03929 NCTC9010_04826 NCTC9037_04708 NCTC9045_05465 NCTC9050_02741 NCTC9062_03523 NCTC9077_05824 NCTC9701_04921 NCTC9775_03044 NCTC9777_01046 RK56_017030 RX35_03830 SAMEA3472055_01598 SAMEA3472067_03766 SAMEA3472108_01690 SAMEA3472114_02034 SAMEA3472147_04808 SAMEA3484427_03836 SAMEA3484429_02481 SAMEA3752559_00279 SAMEA3753064_01454 SAMEA3753290_01943 SAMEA3753300_00780] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dus_3 dus_1 dus_4 dusA NCTC8849_00373 NCTC9637_06214 SAMEA4364652_04714] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA CF386_04175] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA A3E83_08295] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dus_3 dus_2 dusA ERS013166_04148 ERS013197_05893 ERS013201_03751] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[yjbN dusA SSPSH_002147] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dus_2 dusA yjbN DFS07_10651 F01_190101 NCTC13227_05232 UE95_33090] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dus dus_2 dus_3 dusA C9J66_17745 ERS013138_03006 ERS013140_01998 ERS013173_03823 ERS013198_03540 ERS013199_03147 ERS013200_03948 ERS013202_03659 ERS013262_02014] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA PA2795] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dus dusA clem_06730] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA BLE401_12355] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dus_3 dus_2 dusA NCTC10262_04941 NCTC9177_01451 SM87_00086] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[clcA dusA NCTC10722_02243] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA A3J38_09915] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA CBC09_07800] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA CMQ17_13760] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA CMQ49_06840] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dus_1 dusA CO731_03229] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA VC_0379] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA SO_3912] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dus_3 dusA] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dus_2 dusA BV133_2400 BVIRIDIS_17600] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA Rvan_1748] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA swp_0730] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)
[dusA STM4243] tRNA-dihydrouridine(20/20a) synthase (EC 1.3.1.-) (EC 1.3.1.91) (U20-specific dihydrouridine synthase) (U20-specific Dus) (tRNA-dihydrouridine synthase A)

Bibliography :
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