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IMMsII _ Immunization grade mouse type II collagen
/Product Detail : 20061 IMMsII _ Immunization grade mouse type II collagen
Product name : IMMsII _ Immunization grade mouse type II collagen
Catalog number : 20061
Quantity: 1 mg lyophilized
Supplier name : Chondr
Data sheet : Ask more or other datasheet now !
DESCRIPTION: Highly purified type II collagen (treated with 3M guanidine, DEAE-cellulose and Na2HPO4)
APPLICATION: Can be used for various purposes, such as a standard for collagen analysis by SDS-gel, substrate for MMP-1, MMP-8 and MMP-13, and as an antigen for immunizing animals for the induction of
collagen-induced arthritis (CIA) in certain species and strains of experimental animals.
QUANTITY: 1 mg
FORM: Lyophilized powder
SOURCE: Mouse sternal cartilage
MOLECULAR WEIGHT: 300 Kd
PURITY: >99% by SDS-PAGE, free of pepsin and proteoglycans
SOLUBILITY: Soluble in acidic buffer (maximum 4 mg/mL), but difficult to dissolve in neutral buffer. In order to dissolve this product in a neutral buffer, use 0.01M or 0.05M acetic acid to dissolve the collagen at 1-4 mg/mL and then add this collagen solution to a desired neutral buffer such as 2-10X Tris-NaCl (the final concentration of NaCl: 0.15-0.2M) or dialyze against 1X neutral buffer at 4°C.
Note 1: To avoid fibril formation under neutral conditions, keep solution on ice.
Note 2: The physicochemical property of type II collagen differs from type I collagen, and type II collagen
dissolved in a neutral buffer does not form stable gel with incubation at 37°C. In order to prepare
a type II collagen gel, additional components such as type I collagen or other substances might
STORAGE TEMPERATURE: 4°C in the dark
STABILITY: 2 years
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