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Index / Bioner / AccuPower RT_PCR PreMix thin_wall 96_well full_skirted plate /Product Detail : K-2262-2 AccuPower RT_PCR PreMix thin_wall 96_well full_skirted plate
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#K-2262-2 AccuPower RT_PCR PreMix thin_wall 96_well full_skirted plate

 Price: 318   EUR
361   USD
247   GBP

Product name : AccuPower RT_PCR PreMix thin_wall 96_well full_skirted plate

Catalog number : K-2262-2

Quantity: 10 ul

Availability: Yes

Supplier name : Bioner

Data sheet Data sheet : Ask more or other datasheet now !

About this Product :

AccuPower RT_PCR PreMix thin_wall 96_well full_skirted plate PCR kit use certain PCR kits can be used for diagnostics. We supply CE approved PCR kits or research PCR kits. You can request more information about the use of AccuPower RT_PCR PreMix thin_wall 96_well full_skirted plate to [email protected]
http://mybiofast.com/ver.php?search=pcr&submit=Search

More Details about

 

Specifications

5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: No
Fragment size: 6 kb


Application

cDNA synthesis followed by PCR



figure1



Figure 1. Specific amplification of 5' - UTR region of HCV with AccuPower® RT-PCR PreMix.
Human DNA was amplified to generate 0.1-1.5 kb PCR fragments using 1 U of polymerase. The comparison was performed under the same experimental conditions.


Lane 1: DNA Ladder (BM corp.)
Lane 2: Negative control
Lane 3, 4: HCV positive serum
Lane 5: HCV negative serum


figure2



Figure 2. Sensitivity comparison between AccuPower® RT/PCR PreMix and Supplier I RT-PCR Kit
Each 109 copy – 103 copy of PSTVd used for RT/PCR and the same amount of RT/PCR products used for electrophoresis


Lane1: 109 copy     Lane2: 108 copy
Lane3: 107 copy     Lane4: 106 copy
Lane5: 105 copy     Lane6: 104 copy
Lane7: 103 copy     Lane8: NTC


figure3



Figure 3. Comparison of reproducibility test for AccuPower RT/PCR PreMix batch 1,2,3 and batch 4 products using serial diluted Human Total RNA. 
Each 109 copy – 103 copy of PSTVd used for RT/PCR and the same amount of RT/PCR products used for electrophoresis


Lane 1: 10 ng Human total RNA from HeLa cell 
Lane 2: 1 ng Human total RNA from HeLa cell
Lane 3: 100 pg Human total RNA from HeLa cell
Lane 4: 10 pg Human total RNA from HeLa cell

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