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AccuPower GreenStar qPCR PreMix Exicycler 8_well strip, 12 strips _ 96 tests, optical film included
/Product Detail : K-6210 AccuPower GreenStar qPCR PreMix Exicycler 8_well strip, 12 strips _ 96 tests, optical film included
Product name : AccuPower GreenStar qPCR PreMix Exicycler 8_well strip, 12 strips _ 96 tests, optical film included
Catalog number : K-6210
Quantity: 20 ul
Supplier name : Bioner
Data sheet : Ask more or other datasheet now !
5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: Yes
Fragment Size: ~1 kb
- Real-Time Quantification of DNA/cDNA targets.
- Quantification of gene expression
- Microbial/Viral Pathogen Detection.
Figure 1. Real-Time PCR Data of AccuPower® Greenstar™ qPCR PreMix
AccuPower® Greenstar™ PreMix provides at least 7 orders of magnitude in dynamic range (10 fg ~ 10 ng /reaction).
A : Amplification curve. Lambda DNA primers were added into Greenstar™ qPCR PreMix. A series of Lambda DNA positive control diluents were tested.
B : Standard curve. Data were obtained using Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co.).
C : Melting curve.
Figure 2. Comparison of amplification quality between AccuPower® Greenstar™ qPCR PreMix and other supplier's master mixture.
Amplification & Standard curve of AccuPower® Greenstar™ qPCR PreMix and other supplier’s master mixture.
Lambda DNA primers were added into AccuPower® Greenstar™ qPCR PreMix and other supplier’s master mixture. A series of Lambda DNA positive control diluents were tested. Reaction mixtures were prepared and qPCR was performed according to each supplier’s protocol.
All data were obtained using ABI 7500 Fast Real-Time PCR machine (Applied Biosystems co.).
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
The lyophilized composition of this product is US Patent Nos. 5861251 and 6153412. Certain applications of this product are covered by pending or issued patents in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used. Products may be covered by pending or issued patents. Please contact us for more information.
Note: 'Top DNA Polymerase' of this product is specifically optimized for increasing base incorporation rate by inactivating 5'->3' exonuclease activity. Therefore, this is not recommended to use for Real Time PCR using Taqman Probe, for whichAccuPower® Greenstar™ qPCR PreMix.
Trademark: 'AccuPower®' is a registered trademark of Bioneer Corporation. 'DualStar™' is a trademark of Bioneer Corporation.
* Some of the products may not be available in some countries, For details, please contact your local distributor.
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