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Index / Bioner / AccuPower Multiplex PCR PreMix NEW 0.2 ml thin_wall 8_strip tubes with attached cap _ 96 tubes /Product Detail : K-2111 AccuPower Multiplex PCR PreMix NEW 0.2 ml thin_wall 8_strip tubes with attached cap _ 96 tubes
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AccuPower Multiplex PCR PreMix NEW 0.2 ml thin_wall 8_strip tubes with attached cap _ 96 tubes

 Price: 138   EUR
156   USD
107   GBP

Product name : AccuPower Multiplex PCR PreMix NEW 0.2 ml thin_wall 8_strip tubes with attached cap _ 96 tubes

Catalog number : K-2111

Quantity: 20 ul

Availability: Yes

Supplier name : Bioner

Data sheet Data sheet : Ask more or other datasheet now !

About this Product :

AccuPower Multiplex PCR PreMix NEW 0.2 ml thin_wall 8_strip tubes with attached cap _ 96 tubes PCR kit use certain PCR kits can be used for diagnostics. We supply CE approved PCR kits or research PCR kits. You can request more information about the use of AccuPower Multiplex PCR PreMix NEW 0.2 ml thin_wall 8_strip tubes with attached cap _ 96 tubes to [email protected]
http://mybiofast.com/ver.php?search=pcr&submit=Search

More Details about

 

Specifications

5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: Yes
Fragment Size: ~1 kb


Application

Product Name 5' to 3' exonuclease
Human and Animal STR analysis for determining genetic profiles in forensic cases
Molecular diagnostic analysis
Genotyping assay
Qualitative and semi-qualitative gene expression assay
Mutant screening
Transgenic organism analysis

figure1



Figure 1. Single PCR and Multiplex PCR using AccuPower® Multiplex PCR PreMix

Each lane from left to right indicates the single and Multiplex PCR product using AccuPower® Multiplex PCR PreMix.
a) 10plex Multiplex PCR, b) 20plex Multiplex PCR
M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020)


figure2



Figure 2. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit.

6-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit. A series of Human genomic DNA diluents were tested. ( Lane 1. Human genomic DNA 100ng, Lane 2. Human genomic DNA 10ng, Lane 3. Human genomic DNA 1ng ). All data were obtained using Mygene 96 machine (Applied Bioneer co.)
Supplier Q : Q company Multiplex PCR kit, Supplier S : S company Multiplex PCR kit, Supplier I : I company Taq polymerase for Mutiplex PCR (0.5U, added 2mM MgCl2) Rxn. Condition : 95°C for 10min, followed by 35cycles of 30sec at 95°C, 30sec at 60°C, 60sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020)


figure3



Figure 3. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit.

10-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit. A series of Human genomic DNA diluents were tested. ( Lane 1. Human genomic DNA 100ng, Lane 2. Human genomic DNA 10ng, Lane 3. Human genomic DNA 1ng ). All data were obtained using Mygene 96 machine (Applied Bioneer co.)
Supplier Q : Q company Multiplex PCR kit, Supplier S : S company Multiplex PCR kit, Supplier I : I company Taq polymerase for Mutiplex PCR (0.5U, added 2mM MgCl2)Rxn. Condition : 95°C for 10min, followed by 35cycles of 30sec at 95°C, 30sec at 65°C, 60sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020)


figure4



Figure 4. Comparison of amplification quality using Lapchip® between AccuPower® Multiplex PCR PreMix and other supplier’s master mixture.

a) Virtual Gel Image. Gel image illustrates data reproducibility of the LabChip 90 system.
b) Overlay of expression level using Bioneer’s multiplex PCR PreMix and other company’s multiplex PCR kit. The electropherogram displays the data between 10 PCR products yield using 10-plex primer sets to illustrate the Amplification efficiency.
c) The graph shows the total concentration of PCR products between AccuPower® Multiplex PCR PreMix and other supplier’s master mixture.


figure5



Figure 5. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit.

20-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier’s Multiplex PCR kit. A series of Human genomic DNA diluents were tested. (100ng ~ 1ng). All data were obtained using Mygene 96 machine (Applied Bioneer co.)
Supplier Q : Q company Multiplex PCR kit, Supplier S : S company Multiplex PCR kit, Supplier I : I company Taq polymerase for Mutiplex PCR (0.5U, added 2mM MgCl2)
Rxn. Condition : 95°C for 10min, followed by 35cycles of 30sec at 95°C, 30sec at 57°C, 60sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020) Trademark: 'AccuPower®' is a registered trademark of Bioneer Corporation.

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