Product name : AccuPrep PCR Purification Kit
Catalog number : K-3034
Quantity: 200 purifications
Supplier name : Bioner
Data sheet : Ask more or other datasheet now !
AccuPrep® PCR Purification Kit is designed for the purification of up to 10 ug of DNA fragment from PCR and other enzymatic products within 5 minutes. The size range for effective purification is 100 bp ~10 kb, thus common 20 ~ 40 mer oligonucleotides are removed. The recovery yield exceeds 70~90%. Elution volume can be as little as 30 uL when concentrated product is needed.
Completely purify the fragment DNA from various enzymatic reaction products within 5 min.
Highly purified and high yield fragment DNA can be purified from various enzymatic reaction products (restriction enzyme digestion, A-tailing, labeling…).
Double strand and single strand DNA can be purified with high recovery.
Usable range is 100bp to 10kb with 70%-90% recovery.
Silica based DNA binding column with high binding efficiency.
|Components||K-3034 (200 reaction)||K-3034-1 (50 reaction)|
|Buffer ① (PCR Binding buffer)||120 ml X 1 ea||25 ml X 1 ea|
|Buffer ② (Wahsing buffer)||25 ml X 2 ea||6 ml X 2 ea|
|Buffer ③ (Elution buffer)||15 ml X 1 ea||15 ml X 1 ea|
|DNA binding column tube||50 ea X 4 box||50 ea X 1 box|
|Manual||1 ea||1 ea|
|One Page Protocol||1 ea||1 ea|
Sub-cloning, Sequencing, Labeling, DNA concentration, etc
Fig. 1 Typical recovery
The figure shows recovery percentage after purification of DNA amplified by PCR.
The 70 % - 90 % of DNA were recovered regardless of DNA size(0.2 - 6.0 kb).
B; AccuPrep® PCR Purification Kit (K-3034, K-3034-1), Q; Competitor
Fig. 2 Recovery analysis of the purified PCR product
Lane M; Molecular weight marker, 100 bp Plus ladder (D-1035, Bioneer),
Lane 100 ~ 50; control DNA(100% ~ 50%),
Lane B; purified PCR product
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