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Index / MarkerGene / MarkerGeneTM in vivo lacZ beta_Galactosidase Intracellular Detection Kit, Allows ultra_sensitive detection of ß_galactosidase activity in live mammalian, yeast, bacterial, or plant cells. Especially u /Product Detail : M0259 MarkerGeneTM in vivo lacZ beta_Galactosidase Intracellular Detection Kit, Allows ultra_sensitive detection of ß_galactosidase activity in live mammalian, yeast, bacterial, or plant cells. Especially u
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MarkerGeneTM in vivo lacZ beta_Galactosidase Intracellular Detection Kit, Allows ultra_sensitive detection of ß_galactosidase activity in live mammalian, yeast, bacterial, or plant cells. Especially u

 Price: 383   EUR
434   USD
297   GBP

Product name : MarkerGeneTM in vivo lacZ beta_Galactosidase Intracellular Detection Kit, Allows ultra_sensitive detection of ß_galactosidase activity in live mammalian, yeast, bacterial, or plant cells. Especially u

Catalog number : M0259

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Supplier name : MarkerGene

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Description:
Allows ultra-sensitive detection of β-galactosidase activity in live mammalian, yeast, bacterial, or plant cells. Especially useful for FACS analysis.

Application:
One of the most common reporter genes used in molecular biology applications is the E.Coli lacZ gene that codes for an active subunit of beta-galactosidase in vivo. Since this enzyme is generally absent in normal mammalian cells, it can be detected at very low levels, and since the enzyme has a wide substrate specificity, monitoring lacZ expression (and therefore co-expressed genes or promoter efficiency) has become routine to the point of detection of as few as 5 copies of beta-galactosidase per cell.

Although chromogenic assays of beta-galactosidase activity (i.e. X-Gal) have use, the recent application of the fluorogenic substrate fluorescein di-β-D-galactopyranoside (FDG) combined with fluorescence microscopic analysis (confocal microscopy) analysis has been shown to be several orders of magnitude more sensitive. In addition, because of its improved detection limits, the FDG substrate has found extensive use in automated ELISA type assay systems .

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MarkerGene \ MarkerGeneTM_in_vivo_lacZ_beta_Galactosidase_Intracellular_Detection_Kit,_Allows_ultra_sensitive_detection_of_ß_galactosidase_activity_in_live_mammalian,_yeast,_bacterial,_or_plant_cells._Especially_u \ M0259
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