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Index / Biolin / SensiFAST SYBR Hi_ROX One_Step Kit /Product Detail : BIO-73001 SensiFAST SYBR Hi_ROX One_Step Kit
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SensiFAST SYBR Hi_ROX One_Step Kit

 Price: 156   EUR
177   USD
121   GBP

Product name : SensiFAST SYBR Hi_ROX One_Step Kit

Catalog number : BIO-73001

Quantity: 100 rxns

Availability: Yes

Supplier name : Biolin

Data sheet Data sheet : Ask more or other datasheet now !

More Details about

 
"After getting nowhere with other kits we tried the SensiFAST SYBR One-Step Kit and saw results first time, I cannot describe how wonderful this made us feel."

- Asia-Pacific Special Nutrients Sdn. Bhd.
 


Features and Benefits
 
  • Rapid - hot start capability saves time and reduces primer-dimer formation
  • Unique buffer chemistry - for highest specificity and sensitivity
  • Accurate quantification - from low copy targets
  • Sensitive – unique buffer chemistry for highest specificity

 
Instrument Compatibility

ABI 7000, 7300, 7700, 7900, 7900HT, StepOne™, StepOne™ Plus, (See product selection table), each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.
 
Description

The SensiFAST SYBR® Hi-ROX One-Step Kit has been developed for fast RT-qPCR and has been validated on several commonly used real-time instruments. The SensiFAST SYBR® Hi-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR® Hi-ROX One-Step Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR (Fig. 1).

The SensiFAST SYBR® Hi-ROX One-Step Kit consists of a 2x SensiFAST SYBR® One-Step mix, which contains ROX for optional use, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.

 
Volume

BIO-73001: 100 x 20µl Reactions: 1 x 1ml
BIO-73005: 500 x 20µl Reactions: 5 x 1ml

 
Components

2x Reaction mix consisting of:
  • Heat-activated DNA polymerase
  • Ultra-pure dNTPs
  • MgCl2
  • ROX
  • SYBR® Green I

Plus separate vials of:
  • Reverse transcriptase
  • RNase inhibitor
  • DEPC-treated water

 
Kit Size

The kit size is based on a 20µl final reaction volume.
 
Storage Conditions

6 months at -20°C. Avoid exposure of the SYBR® Green I to light.

 

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