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Index / Biolin / SensiFAST SYBR & Fluorescein One_Step Kit /Product Detail : BIO-75001 SensiFAST SYBR & Fluorescein One_Step Kit
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SensiFAST SYBR & Fluorescein One_Step Kit

 Price: 156   EUR
177   USD
121   GBP

Product name : SensiFAST SYBR & Fluorescein One_Step Kit

Catalog number : BIO-75001

Quantity: 100 rxns

Availability: Yes

Supplier name : Biolin

Data sheet Data sheet : Ask more or other datasheet now !

More Details about

Features and Benefits
 
  • Accurate quantification - of gene expression over a broad dynamic range
  • Sensitive – unique buffer chemistry for highest specificity
  • Flexible - suitable for block-based instruments where fluorescein is required to calculate dynamic well factors
  • Convenient - Fast and easy one-tube setup

 
Instrument Compatibility

Bio-Rad iCycler®, iQ™5, MyiQ™, (See product selection table), each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of fluorescein.
 
Description

The SensiFAST SYBR® & Fluorescein One-Step Kit has been developed for fast RT-qPCR and has been validated on several BioRad real-time PCR instruments. The SensiFAST SYBR® & Fluorescein One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR® & Fluorescein One-Step Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR (Fig. 1).

The SensiFAST SYBR® & Fluorescein One-Step Kit consists of a 2x SensiFAST SYBR® One-Step mix, which contains fluorescein for optional use, separate reverse transcriptase and RiboSafe RNase Inhibitor.

 
Volume

BIO-75001: 100 x 20µl Reactions: 1 x 1ml
BIO-75005: 500 x 20µl Reactions: 5 x 1ml

 
Components

2x Reaction mix consisting of:
  • Heat-activated DNA polymerase
  • Ultra-pure dNTPs
  • MgCl2
  • Fluorescein
  • SYBR® Green I

Plus separate vials of:
  • Reverse transcriptase
  • RNase inhibitor
  • DEPC-treated water

 
Kit Size

The kit size is based on a 20µl final reaction volume.
 
Storage

6 months at -20°C. Avoid exposure of the SYBR® Green I to light.

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