Accurate quantification - of gene expression over a broad dynamic range
Sensitive – unique buffer chemistry for highest specificity
Flexible - suitable for block-based instruments where fluorescein is required to calculate dynamic well factors
Convenient - Fast and easy one-tube setup
Bio-Rad iCycler®, iQ™5, MyiQ™, (See product selection table), each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of fluorescein.
The SensiFAST SYBR® & Fluorescein One-Step Kit has been developed for fast RT-qPCR and has been validated on several BioRad real-time PCR instruments. The SensiFAST SYBR® & Fluorescein One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR® & Fluorescein One-Step Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR (Fig. 1).
The SensiFAST SYBR® & Fluorescein One-Step Kit consists of a 2x SensiFAST SYBR® One-Step mix, which contains fluorescein for optional use, separate reverse transcriptase and RiboSafe RNase Inhibitor.
BIO-75001: 100 x 20µl Reactions: 1 x 1ml
BIO-75005: 500 x 20µl Reactions: 5 x 1ml
2x Reaction mix consisting of:
Heat-activated DNA polymerase
SYBR® Green I
Plus separate vials of:
The kit size is based on a 20µl final reaction volume.
6 months at -20°C. Avoid exposure of the SYBR® Green I to light.