Rapid - Optimized for fast reverse transcription real-time PCR
Accurate quantification - For RNA from low copy targets
Sensitive – unique buffer chemistry for highest specificity
Flexible - Compatible with all fast cycling instruments
Roche LightCycler® 480 platform, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, 6000, Eppendorf Mastercycler® ep realplex and Techne Quantica®. (See product selection table).
The SensiFAST™ Probe No-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube (Fig. 1). The kit is designed for use with probe-detection technology (Fig. 2), including TaqMan®, Scorpions® and molecular beacon probes (Fig. 3). A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system ensures that SensiFAST Probe No-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step RT-qPCR.
The SensiFAST Probe No-ROX One-Step Kit consists of a 2x SensiFAST Probe No-ROX One-Step mix, separate reverse transcriptase and RiboSafe RNase Inhibitor.
BIO-76001: 100 x 20µl Reactions: 1 x 1ml
BIO-76005: 500 x 20µl Reactions: 5 x 1ml
2x Reaction mix consisting of:
Heat-activated DNA polymerase
Plus separate vials of:
The kit size is based on a 20µl final reaction volume.