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Index / Biosera / Nanomycopulitine Concentrat 20 x _ 100 ml /Product Detail : LM-A4116/100 Nanomycopulitine Concentrat 20 x _ 100 ml
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#LM-A4116/100 Nanomycopulitine Concentrat 20 x _ 100 ml

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  Price : 267   EUR
303   USD
207   GBP
1123   Zloty
35785   JPY
2062   NOK
2210   SEK
302   CHF

Product name : Nanomycopulitine Concentrat 20 x _ 100 ml

Catalog number : LM-A4116/100

Quantity: 100 ml

Availability: Yes

Supplier name : Biosera

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More Details about

1. Intended use
Continuous use of sterile cell culture equipment, media and cells, leads to long-term microbial contamination risk. A number of research and industrial applications involve antibiotics or toxins to reduce this risk. Applications where non-sterile cells were taken into cell culture are dependent of supplements suppressing contaminants. There are also circumstances where physical contaminant removal is inappropriate because filtration compromises the component viability or activity.
The toxin includes different required advantages for a suitable application in routine cell culture:
-The NanomycopulitineR preferably eliminates bacteria.
-It is active and kills bacteria in both, logarithmic and stationary growing life cycle phases.
-The toxin does not affect the normal metabolism or enzymatic function.
-The solutions do not contain excipients or buffer which influence the growth negatively.
-The toxin is stable in liquid form under normal storage conditions and also in cell culture to provide protection over a period of time
-NanomycopulitineR has a wide spectrum of activity against gram negative and gram positive bacteria nd also others like Chlamydia,
Mycobacteria, a broad range of Mycoplasma, Nanobacteria and bacteria L-forms.
-It is non toxic and safe with respect to human handling
2. Application
NanomycopulitineR can be used in vitro to clean already contaminated cell cultures and to prevent recontamination during further cultivation.
Curing of Mycoplasma infected cell lines is one of the most important applications of this innovative agent. Another useful
NanomycopulitineR application is media protection against contamination if sterile filtration is not appropriate.
3. Procedure
-This test is designed for 1 ml concentrated NanomycopulitineR 20X concentrated solution
Use a 24 well plate
To prepare the NanomycopulitineR with a 1/20 as dilution in your regular cell culture medium ( It could be 1/15, 1/25 or 1/30
depending on the cell type)
-Resuspend 5 x 104 cells in 1 ml of the selected dilution
-Seed the cells and grow them for several days
-Check growth and morphology to compare them with your control culture
Notice: In between Nanomycopulitinal treatments, there should be cultivation without the addition of NanomycopulitineR to devoid resistant bacterial forms.
4. Sterility
Filter sterilized by 0.2 £gm
5. Solution Stability
Stable at 4¢XC for 2 weeks and 24 months as concentrate at ¡V20¢XC

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Pathways :
WP1003: Ovarian Infertility Genes
WP1072: GPCRs, Class A Rhodopsin-like
WP112: Principle Pathways of Carbon Metabolism
WP1120: Ovarian Infertility Genes
WP1152: Mismatch repair
WP1188: GPCRs, Class A Rhodopsin-like
WP1204: Mismatch repair
WP1233: Mismatch repair
WP1257: Mismatch repair
WP1267: Senescence and Autophagy
WP1295: Mismatch repair
WP1305: Senescence and Autophagy
WP1340: Ovarian Infertility Genes
WP1361: Mismatch repair
WP1449: Regulation of toll-like receptor signaling pathway
WP1471: TOR signaling
WP1544: MicroRNAs in cardiomyocyte hypertrophy
WP1575: TOR signaling
WP2002: Epithelium TarBase
WP2003: Leukocyte TarBase
WP2004: Lymphocyte TarBase
WP2005: Muscle cell TarBase
WP2197: Endothelin
WP2291: Deregulation of Rab and Rab Effector Genes in Bladder Cancer
WP258: TGF-beta Receptor Signaling Pathway

Related Genes :
[TY1B-ML1 YMLWTy1-1 POL YML045W YM9827.08] Transposon Ty1-ML1 Gag-Pol polyprotein (Gag-Pol-p199) (TY1A-TY1B) (Transposon Ty1 TYA-TYB polyprotein) (p190) [Cleaved into: Capsid protein (CA) (Gag-p45) (p54); Ty1 protease (PR) (EC 3.4.23.-) (Pol-p20) (p23); Integrase (IN) (Pol-p71) (p84) (p90); Reverse transcriptase/ribonuclease H (RT) (RT-RH) (EC 2.7.7.49) (EC 2.7.7.7) (EC 3.1.26.4) (Pol-p63) (p60)]
[TY1B-ML2 YMLWTy1-2 POL YML039W YM8054.04] Transposon Ty1-ML2 Gag-Pol polyprotein (Gag-Pol-p199) (TY1A-TY1B) (Transposon Ty1 TYA-TYB polyprotein) (p190) [Cleaved into: Capsid protein (CA) (Gag-p45) (p54); Ty1 protease (PR) (EC 3.4.23.-) (Pol-p20) (p23); Integrase (IN) (Pol-p71) (p84) (p90); Reverse transcriptase/ribonuclease H (RT) (EC 2.7.7.49) (EC 2.7.7.7) (EC 3.1.26.4) (Pol-p63) (p60)]
[PMEL D12S53E PMEL17 SILV] Melanocyte protein PMEL (ME20-M) (ME20M) (Melanocyte protein Pmel 17) (Melanocytes lineage-specific antigen GP100) (Melanoma-associated ME20 antigen) (P1) (P100) (Premelanosome protein) (Silver locus protein homolog) [Cleaved into: M-alpha (95 kDa melanocyte-specific secreted glycoprotein) (P26) (Secreted melanoma-associated ME20 antigen) (ME20-S) (ME20S); M-beta]
[ND2 MTND2 NADH2 ndh2 HS5781_0010 HS5782_0010] NADH-ubiquinone oxidoreductase chain 2 (EC 7.1.1.2)
[] Beta-galactoside-specific lectin 3 (Beta-galactoside-specific lectin II) (Beta-galactoside-specific lectin III) [Cleaved into: Beta-galactoside-specific lectin 3 chain A isoform 1 (EC 3.2.2.22) (Beta-galactoside-specific lectin III chain A isoform 1) (Lectin chain A isoform 2) (ML-3 A) (ML-III A) (rRNA N-glycosidase); Beta-galactoside-specific lectin 3 chain B (Beta-galactoside-specific lectin III chain B) (ML-3 B) (ML-III B)]
[MCOLN1 ML4 MSTP080] Mucolipin-1 (ML1) (MG-2) (Mucolipidin) (Transient receptor potential channel mucolipin 1) (TRPML1)
[] Beta-galactoside-specific lectin 4 (Beta-galactoside-specific lectin IV) [Cleaved into: Beta-galactoside-specific lectin 4 chain A (EC 3.2.2.22) (Beta-galactoside-specific lectin IV chain A) (ML-4 A) (ML-IV A) (rRNA N-glycosidase); Beta-galactoside-specific lectin 4 chain B (Beta-galactoside-specific lectin IV chain B) (ML-4B) (ML-IV B)]
[env] Envelope glycoprotein gp160 (Env polyprotein) [Cleaved into: Surface protein gp120 (SU) (Glycoprotein 120) (gp120); Transmembrane protein gp41 (TM) (Glycoprotein 41) (gp41)]
[TPX2 C20orf1 C20orf2 DIL2 HCA519] Targeting protein for Xklp2 (Differentially expressed in cancerous and non-cancerous lung cells 2) (DIL-2) (Hepatocellular carcinoma-associated antigen 519) (Hepatocellular carcinoma-associated antigen 90) (Protein fls353) (Restricted expression proliferation-associated protein 100) (p100)
[Pre C,C C core E PC pre-C pre-C/C PreC preC PreC/C preC/C precore-core HBVgp4] Capsid protein (Core antigen) (Core protein) (HBcAg) (p21.5)
[mlcC] Dihydro-ML-236C monooxygenase mlcC (EC 1.14.14.-) (Compactin biosynthesis protein C) (DihydroML-236B hydroxylase)
[pfp xcc-b100_0891] Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (EC 2.7.1.90) (6-phosphofructokinase, pyrophosphate dependent) (PPi-dependent phosphofructokinase) (PPi-PFK) (Pyrophosphate-dependent 6-phosphofructose-1-kinase)
[] Beta-galactoside-specific lectin 1 (Beta-galactoside-specific lectin I) (Viscumin) [Cleaved into: Beta-galactoside-specific lectin 1 chain A isoform 1 (EC 3.2.2.22) (Beta-galactoside-specific lectin I chain A isoform 1) (ML-I A) (MLA) (rRNA N-glycosidase); Beta-galactoside-specific lectin 1 chain B (Beta-galactoside-specific lectin I chain B) (ML-I B) (MLB)]
[flu yeeQ yzzX b2000 JW1982] Antigen 43 (AG43) (Fluffing protein) [Cleaved into: Antigen 43 alpha chain; Antigen 43 beta chain]
[ND4L MTND4L NADH4L HS5781_0027 HS5782_0027] NADH-ubiquinone oxidoreductase chain 4L (EC 7.1.1.2)
[MHR1 XTC1 YDR296W] Mitochondrial homologous recombination protein 1 (Cross-linked transcription component 1) (Mitochondrial large ribosomal subunit protein mL67)
[S100B] Protein S100-B (S-100 protein beta chain) (S-100 protein subunit beta) (S100 calcium-binding protein B)
[NUP100 NSP100 YKL068W YKL336] Nucleoporin NUP100/NSP100 (Nuclear pore protein NUP100/NSP100)
[CYTB CTYB CYB CytB cytb] Cytochrome b
[SP100] Nuclear autoantigen Sp-100 (Nuclear dot-associated Sp100 protein) (Speckled 100 kDa)
[S100A12] Protein S100-A12 (CGRP) (Calcium-binding protein in amniotic fluid 1) (CAAF1) (Calgranulin-C) (CAGC) (Extracellular newly identified RAGE-binding protein) (EN-RAGE) (Migration inhibitory factor-related protein 6) (MRP-6) (p6) (Neutrophil S100 protein) (S100 calcium-binding protein A12) [Cleaved into: Calcitermin]
[MED24 ARC100 CRSP4 DRIP100 KIAA0130 THRAP4 TRAP100] Mediator of RNA polymerase II transcription subunit 24 (Activator-recruited cofactor 100 kDa component) (ARC100) (Cofactor required for Sp1 transcriptional activation subunit 4) (CRSP complex subunit 4) (Mediator complex subunit 24) (Thyroid hormone receptor-associated protein 4) (Thyroid hormone receptor-associated protein complex 100 kDa component) (Trap100) (hTRAP100) (Vitamin D3 receptor-interacting protein complex 100 kDa component) (DRIP100)
[S100A1 S100A] Protein S100-A1 (S-100 protein alpha chain) (S-100 protein subunit alpha) (S100 calcium-binding protein A1)
[S100A11 MLN70 S100C] Protein S100-A11 (Calgizzarin) (Metastatic lymph node gene 70 protein) (MLN 70) (Protein S100-C) (S100 calcium-binding protein A11) [Cleaved into: Protein S100-A11, N-terminally processed]
[S100P S100E] Protein S100-P (Migration-inducing gene 9 protein) (MIG9) (Protein S100-E) (S100 calcium-binding protein P)
[aceA icl b4015 JW3975] Isocitrate lyase (ICL) (EC 4.1.3.1) (Isocitrase) (Isocitratase)
[S100B] Protein S100-B (S-100 protein beta chain) (S-100 protein subunit beta) (S100 calcium-binding protein B)
[S100b] Protein S100-B (S-100 protein beta chain) (S-100 protein subunit beta) (S100 calcium-binding protein B)
[S100A2 S100L] Protein S100-A2 (CAN19) (Protein S-100L) (S100 calcium-binding protein A2)
[S100A3 S100E] Protein S100-A3 (Protein S-100E) (S100 calcium-binding protein A3)

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