The CD51X Dual Promoter Series
High level of expression from either the CMV, UbC or MSCV promoter. The multiple cloning site (MCS) located downstream of a promoter allows for convenient cloning of your gene of interest. The SV40 polyadenylation signal in both vectors enables efficient termination of transcription, resulting in high levels of steady-state mRNA. Downstream of your expression cassette is an EF1 promoter driving the expression of either the Puro, GFP, RFP, Neo or Hygro marker genes in the Single marker options.
Lentivector CD515B-1 MCS does not have a unique EcoRI site, as there is an additional EcoRI site in the Hygro marker gene.
SBI also offers a Dual marker cDNA expression vector that features both GFP or RFP along with Puro markers that are co-expressed (GFP-Puro, cat# CD513B-1 and CD550A-1, RFP-Puro, cat# CD516B-2). The CD550A-1 lentivector features an additional BGH poly-A site in between the EF1 promoter and the downstream PGK promoter to enhance EF1-driven expression of your cDNA clone.
The CD52X EF1 Promoter Series with T2A Co-expressed markers
SBI’s cDNA expression vectors using the EF1 promoter driving expression also incorporates the 2A-like sequence (T2A) from the insect virus Thosea asigna to mediate the co-expression of a reporter gene with the target cDNA. Reporter genes have been cloned at either the first or second positions relative to the T2A element, and both markers achieved high expression levels at either position.
The CD53X EF1 Promoter Series with IRES Co-expressed markers
SBI’s cDNA expression vectors using the EF1 promoter driving expression also incorporates the Viral IRES (Internal Ribosomal Entry Site) sequence to mediate the co-expression of a reporter gene with the target cDNA. Reporter genes have been cloned at either the first or second positions relative to the IRES element, and both markers achieved high expression levels at either position.
MSCV Promoter Series
The MSCV CpG-deficient promoter is the 5’-LTR promoter of murine stem cell virus that is incorporated into the 3’ HIV LTR of the cDNA expression lentivector. After integration into genomic DNA, the 3’MSCV/LTR will replace the 5’LTR and provide a high level of expression of the target gene and the downstream reporter gene. This promoter configuration has durable activity in most hematopoietic, embryonic and stem cells.
—CMV promoter: promotes a high level of expression of your gene of interest in a wide variety of cell lines.
—Multiple Cloning Site (MCS): for cloning the gene of interest in MCS located downstream of CMV promoter.
—Hybrid CMV-5LTR promoter: provides a high level of expression of the full-length viral transcript in producer 293 cells.