Product name : CASPASE_3 ENZYME SUBSTRATE NucViewTM 488
Catalog number : 10400
Supplier name : Biotium
Data sheet : Ask more or other datasheet now !
NucViewTM 488 Caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time.
Unlike conventional caspase assays, NucViewTM 488 Caspase-3 substrate detects caspase-3/7 activity within individual intact cells without inhibiting caspase activity. The substrate consists of a fluorogenic DNA dye and a DEVD substrate moiety specific for caspase-3/7. The substrate, which is both non-fluorescent and nonfunctional as a DNA dye, rapidly crosses cell membranes to enter the cytoplasm, where it is cleaved by caspase-3 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the NucViewTM 488 caspase-3 substrate is bi-functional, allowing detection of intracellular caspase-3/7 activity and visualization of changes in nuclear morphology during apoptosis. The fluorescent staining produced in response to caspase-3/7 activity is compatible with subsequent fixation and permeabilization for immunostaining.
In addition to stand-alone substrate stock solutions, we offer three ready-to-use assay kits with detailed application protocols: 1) NucViewTM 488 Caspase-3 Assay Kit for live cells (cat#30029), 2) Dual Apoptosis Assay with NucViewTM 488 caspase-3 substrate and CFTM594-annexin V (cat#30067), 3) NucViewTM 488 and MitoViewTM633 Apoptosis Assay Kit (cat#30062).
NucViewTM enzyme substrate technology is covered by U.S. Patent No. 8,092,784.
|NucView 488 & CF 647 Annexin V|
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