Rapid - hot-start capability saves time and reduces primer-dimer formation
Sensitive – perfect for hard to obtain and low copy number samples
High performance - ideal for multiplexing and gene expression analysis
Fast protocols - use with the new generation of fast cyclers
ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne™, StepOne™ Plus (see product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.
The SensiFAST™ Probe Hi-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly used real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan® (Fig. 1), Scorpions®and molecular beacon probes. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that the SensiFAST Probe Kit delivers fast, highly-specific and ultra-sensitive real-time PCR (Fig. 2).
SensiFAST Probe is provided as a 2x mastermix containing all the components necessary for real-time PCR, including dNTPs, stabilizers and enhancers.
BIO-82002: 250 x 20µl Reactions: 2 x 1ml
BIO-82005: 500 x 20µl Reactions: 5 x 1ml
BIO-82020: 2000 x 20µl Reactions: 20 x 1ml
2x Reaction mix consisting of:
Heat-activated DNA polymerase
ROX Internal reference
The kit size is based on a 20µl final reaction volume.
6 months at -20°C. Avoid exposure of the ROX™ to light.