The SensiFAST™ Probe No-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly used real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan®, Scorpions®and molecular beacon probes (Fig. 1). A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that the SensiFAST Probe Kit delivers fast, highly-specific and ultra-sensitive real-time PCR (Fig. 2).
SensiFAST Probe is provided as a 2x mastermix containing all the components necessary for real-time PCR, including dNTPs, stabilizers and enhancers.
BIO-86002: 250 x 20µl Reactions: 2 x 1ml
BIO-86005: 500 x 20µl Reactions: 5 x 1ml
BIO-86020: 2000 x 20µl Reactions: 20 x 1ml
2x Reaction mix consisting of:
Heat-activated DNA polymerase
The kit size is based on a 20µl final reaction volume.
6 months at -20°C. Avoid exposure of the ROX™ to light.