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Index / Biovis / Active Human Caspase_1100 units /Product Detail : 1081-100 Active Human Caspase_1100 units
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#1081-100

Active Human Caspase_1100 units

 Price: 373   EUR
423   USD
289   GBP

Product name : Active Human Caspase_1100 units

Catalog number : 1081-100

Quantity: 100 units

Availability: Yes

Supplier name : Biovis

Data sheet Data sheet : Ask more or other datasheet now !

About this Product :

Active Human Caspase_1100 units Human samples 80 % of the research is conducted on human samples. Gentaur suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and elisa kit to Human proteins as well as Active Human Caspase_1100 units.
http://mybiofast.com/ver.php?search=human+&submit=Search

More Details about

UNIT DEFINATION:
One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.


FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
SPECIFIC ACTIVITY: > 5000 units/mg


STORAGE CONDITIONS:
The lyophilized caspase-1 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.


DESCRIPTION:
Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form.
The recombinant active human caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA (Cat. #1104-200) or Ac-YVAD-AFC (1103-200). The rh-Caspase has an N-term His-tag and corresponds to amino acids 120-404 of Caspase-1, gene accession# NP_150634.1.


APPLICATIONS AND USAGE:
Active caspase-1 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase-1 assay protocol, please refer to BioVision’s Caspase-1 Fluorometric or Colorimetric Assay Kits (Cat.#: K110 and K111).

 

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