Product name : Active Human Caspase_10_b25 units
Catalog number : 1090B-25
Quantity: 25 units
Supplier name : Biovis
Data sheet : Ask more or other datasheet now !
One unit of the recombinant caspase-10/b is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
SPECIFIC ACTIVITY: 15000 units/mg
PURITY: >90% by SDS-PAGE
The lyophilized caspase-10/b is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.
Caspase-10b (also known as FLICE 2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-10 also exists in cells as an inactive proenzyme. During apoptosis procaspase-10 is processed at aspartate residues to form active caspase-10 which consists of two large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form.
The recombinant active human caspase-10/b was expressed in E. coli. The active caspase-10 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).
APPLICATIONS AND USAGE:
Active caspase10/b is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase assay protocol, please refer to BioVision’s Caspase Fluorometric or Colorimetric Assay Kits.
For Research Use Only! Not to be used in humans.
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