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Index / Biovis / Active Mouse Caspase_1100 units /Product Detail : 1181-100 Active Mouse Caspase_1100 units
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#1181-100 Active Mouse Caspase_1100 units

 Price: 360   EUR
409   USD
279   GBP

Product name : Active Mouse Caspase_1100 units

Catalog number : 1181-100

Quantity: 100 units

Availability: Yes

Supplier name : Biovis

Data sheet Data sheet : Ask more or other datasheet now !

About this Product :

Active Mouse Caspase_1100 units mus musculus murine Active Mouse Caspase_1100 units detects proteins from variouse species most likely human.
http://mybiofast.com/ver.php?search=musculus&submit=Search

More Details about

UNIT DEFINATION:
One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate Ac-YVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.


FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per μl in PBS containing 15% glycerol.
SPECIFIC ACTIVITY: 20000 units/mg


STORAGE CONDITIONS:
The lyophilized caspase-1 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.


DESCRIPTION:
Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form.
The recombinant active mouse caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA (Cat. #1104-200) or Ac-YVAD-AFC (1103-200).


APPLICATIONS AND USAGE:
Active caspase-1 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase-1 assay protocol, please refer to BioVision’s Caspase-1 Fluorometric or Colorimetric Assay Kits (Cat.#: K110 and K111).

 

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