Product name : Blocking Peptide for PARP pAb 50 ug
Catalog number : 3002BP-50
Quantity: 50 ug
Supplier name : Biovis
Data sheet : Ask more or other datasheet now !
IMMUNOGEN: Synthetic peptide corresponding to caspase cleavage site in human PARP (ID# BV-65-66).
100 μg (0.2 mg/ml) affinity purified rabbit anti-PARP polyclonal antibody in phosphatebuffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
SPECIES REACTIVITY: Human
Store at -20°C. For long term storage, aliquot and freeze at –70°C. Avoid repeated freeze/defrost cycles.
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s Nterminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
The polyclonal anti-PARP antibody recognizes the full length (116 kDa) and the cleaved fragment (85 kDa) of human PARP.
APPLICATION AND USAGE:
Western blot analysis (1-4 μg/ml). However, the optimal concentrations should be determined individually.
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