BNP (HRP) - Mouse monoclonal [24C5] to BNP (HRP); Monoclonalhttp://biotech-map.com/aa_search/table_sample.html?q=monoclonal&filter= Description BNP (HRP) - Mouse monoclonal [24C5] to BNP (HRP); Monoclonal is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
More Details about
Details for BNP [24C5] (HRP), Antibody
Human - Not yet tested in other species.
Immunogen: Synthetic peptide - amino acids 11-22 (FGRKMDRISSSS) corresponding to N terminal of Human BNP
Application Note: For ELISA: Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [50E1] to BNP (). For EIA: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Cellular Localization: Secreted
Myeloma: Sp2/0 Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides, see fig. 18). BNP is a 32 amino acid peptide with disulfide bond between the cystein residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity
Function: Acts as a cardiac hormone with a variety of biological actions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. It is thought to play a key role in cardiovascular homeostasis. Helps restore the body\'s salt and water balance. Improves heart function.
Subunit: Interacts with NPR3.
Subcellular Location: Secreted.
Tissue Specificity: Brain and also in atria, but at much lower levels than ANP.
Pharmaceutical: Available under the name Nesiritide (Scios). Used for the treatment of heart failure.
Similarity: Belongs to the natriuretic peptide family.