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Index / Biovis / Caspase_1 Fluorometric Assay Kit200 assays /Product Detail : K110-200 Caspase_1 Fluorometric Assay Kit200 assays
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#K110-200 Caspase_1 Fluorometric Assay Kit200 assays

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  Price : 568   EUR
644   USD
440   GBP
2386   Zloty
76007   JPY
4380   NOK
4694   SEK
642   CHF

Product name : Caspase_1 Fluorometric Assay Kit200 assays

Catalog number : K110-200

Quantity: 200 assays

Availability: Yes

Supplier name : Biovis

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More Details about

Kit Summary: 
• Detection method- Flow cytometry (400 nm excitation filter and 505 nm emission filter)and fluorescence microscopy 
• Sample type- Cell and tissue lysates 
• Species reactivity- Mammalian 
• Kit size- Convenient sizes (25, 100, 200, 400 assays) 
• Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features & Benefits: 
• Simple one-step procedure; takes 1-2 hours 
• Fast and convenient 
• Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.

Kit components: 
• Cell Lysis Buffer 
• 2X Reaction Buffer 
• YVAD-AFC (1mM) 
• DTT (1 M)

Description: 
Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on detection of cleavage of substrate YVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). YVAD-AFC emits blue light (400 nm); upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.

Storage Conditions: 
-20°C

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