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Index / Sakura / Lab Aid /Product Detail : 4014 Lab Aid
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#4014 Lab Aid

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  Price : 560   EUR
636   USD
434   GBP
2354   Zloty
75005   JPY
4323   NOK
4632   SEK
634   CHF

Product name : Lab Aid

Catalog number : 4014

Quantity: 1 each

Availability: Yes

Supplier name : Sakura

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For the cultivation of non-fastodious microorganisms in water, feces and from clinical samples.
 
FORMULA IN g/l
Gelatin Peptone 5.00
Bacteriological Agar 15.00
Beef Extract 3.00
Final pH 6.8 ± 0.2 at 25ºC
 
PREPARATION
Suspend 23 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes. The prepared medium should be stored at 8-15°C. The color is amber, slightly opalescent. The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes, discard the medium.
 
USES
NUTRIENT AGAR is a general purpose medium, not selective but suitable for the cultivation of a wide variety nonfastidious microorganisms. It can be used as a colony count medium in sanitation, medica and industrial bacteriology.
There are many uses for Nutrient Agar in the bacteriological analysis of drinking water, wastewater, milk and other foods. The American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing.
It is also used in the multiplication of microorganisms to produce vaccines and antigens in general, in the tests of sensitivity and resistance, and as a base to prepare an enriched medium by adding ascitic fluid, etc. It is used to grow microorganisms and for subsequent biochemical tests.
The Gelatin peptone and Beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth.
Bacteriological agar is the solidifying agent.
ISO 6579, ISO 19250 and ISO 10273 recommend this medium to obtain presuntive Salmonella and Yersinia isolated colonies respectively. A typical or suspicious colony from each selective medium must be seeded, and then the other four if the first one turns out to be negative. In the case of epidemiological studies, it is recommended to identify at least five colonies. Should there be less than five typical or suspicious colonies on a plate, all the typical or suspicious colonies will be used for confirmation.
Inoculate medium with the test sample and incubate at 35 ± 2°C for 18 – 48 hours. Good growth will appear as translucent colonies.
According ISO 19250 incubate at 36 ± 2°C for 24 ± 3 hours.
 
MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a temperature of 35 ± 2°C and observed after 18 - 48 hours.
 
Microorganisms Growth
Staphylococcus aureus ATCC 25923 Good
Escherichia coli ATCC 25922 Good
Salmonella typhimurium ATCC 14028 Good
Streptococcus pyogenes ATCC 12344 Good
Streptococcus pneumoniae ATCC 6301 Good
*Yersinia enterocolitica ATCC 23715 Good
*According ISO 10273 incubate at 30ºC during 24 hours.
According ISO 19250 incubate at 36 ± 2°C for 24 ± 3 hours.
 
BIBLIOGRAPHY
Greenberg and Cooper Can. Med. Assn. J. 83:143. 1960. Wetmore and Gochenour J. Bact. 72:79, 1956
Norma UNE-EN-ISO 6579. Microbiology of food and animal feeding stuffs -- Horizontal method for the detection of Salmonella spp.
ISO 10273-2003-06 Microbiology of Food and animal feeding stuffs- Horizontal method for the detection of presumptive pathogenic 
Yersinia enterocolitica.
ISO 19250 water quality-detection of Salmonella spp
 
STORAGE
Once opened keep powdered medium closed to avoid hydration.

 

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[APP A4 AD1] Amyloid-beta precursor protein (APP) (ABPP) (APPI) (Alzheimer disease amyloid protein) (Amyloid precursor protein) (Amyloid-beta A4 protein) (Cerebral vascular amyloid peptide) (CVAP) (PreA4) (Protease nexin-II) (PN-II) [Cleaved into: N-APP; Soluble APP-alpha (S-APP-alpha); Soluble APP-beta (S-APP-beta); C99 (Beta-secretase C-terminal fragment) (Beta-CTF); Amyloid-beta protein 42 (Abeta42) (Beta-APP42); Amyloid-beta protein 40 (Abeta40) (Beta-APP40); C83 (Alpha-secretase C-terminal fragment) (Alpha-CTF); P3(42); P3(40); C80; Gamma-secretase C-terminal fragment 59 (Amyloid intracellular domain 59) (AICD-59) (AID(59)) (Gamma-CTF(59)); Gamma-secretase C-terminal fragment 57 (Amyloid intracellular domain 57) (AICD-57) (AID(57)) (Gamma-CTF(57)); Gamma-secretase C-terminal fragment 50 (Amyloid intracellular domain 50) (AICD-50) (AID(50)) (Gamma-CTF(50)); C31]
[Aicda Aid] Single-stranded DNA cytosine deaminase (EC 3.5.4.38) (Activation-induced cytidine deaminase) (AID) (Cytidine aminohydrolase)
[AICDA AID] Single-stranded DNA cytosine deaminase (EC 3.5.4.38) (Activation-induced cytidine deaminase) (AID) (Cytidine aminohydrolase)
[APP] Amyloid-beta A4 protein (ABPP) (APP) (Alzheimer disease amyloid A4 protein homolog) (Amyloid precursor protein) (Amyloid-beta precursor protein) [Cleaved into: N-APP; Soluble APP-alpha (S-APP-alpha); Soluble APP-beta (S-APP-beta); C99 (Beta-secretase C-terminal fragment) (Beta-CTF) (CTF-beta); Amyloid-beta protein 42 (Abeta42) (Beta-APP42); Amyloid-beta protein 40 (Abeta40) (Beta-APP40); C83 (Alpha-secretase C-terminal fragment) (Alpha-CTF) (CTF-alpha); P3(42); P3(40); C80; Gamma-secretase C-terminal fragment 59 (Gamma-CTF(59)); Gamma-secretase C-terminal fragment 57 (Gamma-CTF(57)); Gamma-secretase C-terminal fragment 50 (Amyloid intracellular domain 50) (AICD-50) (AID(50)) (Gamma-CTF(50)); C31]
[ppnP LAB08_0876] Pyrimidine/purine nucleoside phosphorylase (EC 2.4.2.1) (EC 2.4.2.2) (Adenosine phosphorylase) (Cytidine phosphorylase) (Guanosine phosphorylase) (EC 2.4.2.15) (Inosine phosphorylase) (Thymidine phosphorylase) (EC 2.4.2.4) (Uridine phosphorylase) (EC 2.4.2.3) (Xanthosine phosphorylase)
[nnrE nnrD LAB08_4729] Multifunctional fusion protein [Includes: ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC 4.2.1.136) (ADP-dependent NAD(P)HX dehydratase); NAD(P)H-hydrate epimerase (EC 5.1.99.6) (NAD(P)HX epimerase)]
[fadB LAB08_3530] Fatty acid oxidation complex subunit alpha [Includes: 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase (EC 4.2.1.17) (EC 5.1.2.3) (EC 5.3.3.8)]
[Lat2 Lab Ntal Wbscr15 Wbscr5] Linker for activation of T-cells family member 2 (Linker for activation of B-cells) (Membrane-associated adapter molecule) (Non-T-cell activation linker) (Williams-Beuren syndrome chromosomal region 15 protein homolog)
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