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Immunization grade human amnion type V collagen, 1 mg
/Product Detail : 1095 Immunization grade human amnion type V collagen, 1 mg
Product name : Immunization grade human amnion type V collagen, 1 mg
Catalog number : 1095
Supplier name : Chondr
Data sheet : Ask more or other datasheet now !
DESCRIPTION: Human type V collagen purifi ed from pepsin-solubilized amnion by repeating salt precipitation. Amnion type V collagen consisits of two 1 chains and one 2 chain, [1(V)]2 and 2(V).
APPLICATION: Use as an immunizing antigen to generate antibodies, an antigen to detect anti-type V collagen antibodies in ELISA, or as a standard for gel analysis.
QUANTITY: 1 mg
FORM: Lyophilized powder
MOLECULAR WEIGHT: Intact amnion type V collagen: approximately 540 Kd. By 6% gel analysis, amnion type V collagen is separated into two chains: 1(V) and 2(V) (1840 and 1258 amino acid residues) from the top of the gel.
PURITY: >90% by SDS gel analysis
SOLUBILITY: Type V collagen can be dissolved at 4 mg/ml in acidic solution such as 0.01-0.05M acetic acid, pH 3.0-3.3 or 0.15M citrate buffer, pH 3.6 by stirring at 4°C overnight. To neutralize the solution, add 10x neutral buffer containing 1.5M NaCl or dialyze the solution against a neutral buffer.
STORAGE TEMPERATURE: 4°C in the dark for lyophilized form and -20°C for solution. Collagen might be gradually degraded under neutral conditions.
STABILITY: 2 years in lyophilized form
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