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Index / Jena Bio / Red Load Taq Master _ high yield, S pack Master mix for direct gel loading /Product Detail : PCR-106S Red Load Taq Master _ high yield, S pack Master mix for direct gel loading
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Red Load Taq Master _ high yield, S pack Master mix for direct gel loading

 Price: 85   EUR
97   USD
66   GBP

Product name : Red Load Taq Master _ high yield, S pack Master mix for direct gel loading

Catalog number : PCR-106S

Quantity: 100reactions

Availability: Yes

Supplier name : Jena Bio

Data sheet Data sheet : Ask more or other datasheet now !

More Details about

 

Cat. No.  Amount  Size 
 PCR-106S 100 reactions 1 ml 
 PCR-106L 500 reactions 5 ml 
 
For in vitro use only 
Quality guaranteed for 12 months 
 
Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible 
 
5x Red Load Taq Master / high yield (red cap) 
5x conc. master mix of Taq DNA polymerase, dATP, 
dCTP, dGTP, dTTP, (NH4)2SO4, MgCl2, Tween-20, 
Nonidet P-40, red dye, gel loading buffer, stabilizers 
PCR grade water (white cap) 
 
Description 
Red Load Taq Master / high yield contains an 
inherent red dye and allows the direct loading of the 
PCR reaction product onto the gel. It contains all 
reagents required for PCR (except template and 
primer) in a premixed 5x concentrated ready-to-use 
solution. 
The Master Mix is recommended for use in routine 
PCR reactions. It is optimized for high efficiency and 
gives superior amplification results in a broad range 
of reaction conditions with most primer-template pairs. 
Note that the mix is based on a detergent containing 
buffer system not recommended for plate based PCR 
and automated pipetting. 
The enzyme catalyzes the polymerization of 
nucleotides into duplex DNA in 5'→3' direction in the 
presence of magnesium. It also possesses a 5'→3' 
polymerization-dependent exonuclease replacement 
activity but lacks a 3'→5' exonuclease activity. 
 
Recommended PCR assay 
50 µl PCR assay 
10 µl 5x Taq Master Mix red cap 
0.2-1 µM each Primer  
2-50 ng Template DNA  
Fill up to 50 µl PCR grade H2O white cap 
 
Recommended cycling conditions 
Initial 
denaturation 
94°C 2 min 1x 
Denaturation 94°C 30 sec 
Annealing 
1)
 45 - 68°C 30 sec 
Elongation 
2)
 72°C 30 sec - 3 min 
30x 
Final elongation 72°C 2 min 1x

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