More Details about
Cat. No. Amount Size
PCR-108S 100 reactions 1 ml
PCR-108L 500 reactions 5 ml
For in vitro use only
Quality guaranteed for 12 months
Store at -20°C, avoid frequent thawing and freezing
Storage at 4°C for up to 3 months possible
5x Red Load Taq Master (red cap)
5x conc. master mix of Taq DNA polymerase,
dATP, dCTP, dGTP, dTTP, reaction buffer with KCl and
MgCl2, red dye, gel loading buffer, stabilizers
PCR grade water (white cap)
Red Load Taq Master contains an inherent red dye
and allows the direct loading of the PCR reaction
product onto the gel. It contains all reagents required
for PCR (except template and primer) in a premixed
5x concentrated ready-to-use solution.
The Master Mix is recommended for use in routine
PCR reactions. It is optimized for high specificity and
guarantees minimal by-product formation. The mix is
particularly suitable for plate based PCR and
automated pipetting where a detergent free buffer
system is required.
The enzyme catalyzes the polymerization of
nucleotides into duplex DNA in 5'→3' direction in the
presence of magnesium. It also possesses a 5'→3'
polymerization-dependent exonuclease replacement
activity but lacks a 3'→5' exonuclease activity.
Recommended PCR assay
50 µl PCR assay
10 µl 5x Taq Master Mix red cap
0.2-1 µM each Primer
2-50 ng Template DNA
Fill up to 50 µl PCR grade H2O white cap
Recommended cycling conditions
94°C 2 min 1x
Denaturation 94°C 30 sec
45 - 68°C 30 sec
72°C 30 sec - 3 min
Final elongation 72°C 2 min 1x
1) The annealing temperature depends on the melting
temperature of the primers used.
2) The elongation time depends on the length of the fragments to
be amplified. A time of 1 min/kbp is recommended.
For optimal specificity and amplification an individual
optimization of the recommended parameters may be
necessary for each new template DNA and/or primer pair.
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