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Index / ABM / EF1α Promoter Yamanaka Factors Lentivirus Set /Product Detail : G361 EF1α Promoter Yamanaka Factors Lentivirus Set
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EF1α Promoter Yamanaka Factors Lentivirus Set

 Price: 1094   EUR
1242   USD
849   GBP

Product name : EF1α Promoter Yamanaka Factors Lentivirus Set

Catalog number : G361

Quantity: 5 x 200ul

Availability: Yes

Supplier name : ABM

Data sheet Data sheet : Ask more or other datasheet now !

About this Product :

EF1α Promoter Yamanaka Factors Lentivirus Set lentivectors random inserts of lentivectors are considered mot harmfull and the easiers way to get DNA into nulear DNA. More precise piggy back or talen techniques are used if random inserts are not wished. Our EF1α Promoter Yamanaka Factors Lentivirus Set will randomly isert the DNA and since bad inserts are mostly lethal, healthy modofied cells can be studied.
http://antibody-antibodies.com/anti-elisa/Lentiviral.html

More Details about

Vector Refer to Individual Virus Pages
Plasmid Size Refer to individual viruses.
Promoter EF1α
Gene Oct4, Sox2, KLF4, Myc and GFP control virus.
Organism Human
Accession # Q01860, P48431, O43474 and P01106
Description Five Lenti-III-EF1α viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) and a GFP control. Purified ready-to-use high titer lentivirus at 1x107 cfu/mL
Functions Each individual recombinant lentivirus is provided as a VSV-G pseudotyped and concentrated virus stock capable of infecting both dividing and non-dividing cells. The expression of the four transcription factors provided in this set in combinantion with each other has been shown to reprogram adult human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem cell (iPSCs).
Quality Control Viral titer (cfu/mL) is determined by quantitative RT-PCR of viral stock preparation. Transcription factor protein expression are verified by immunocytochemistry and Western Blot analysis of transduced 293 cells. All of ABM’s viral preparations are tested to be free of bacteria and other microbials.
Titer 1x107 cfu/mL
Storage -70°C
Shipping On Dry Ice
References Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi et al. Cell. 2007 Nov 30;131(5):861-72.

Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahaski and Yamanaka. Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10.

 

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